Table 2

Statistical analyses in Figures 5-9 and Extended Data Figure 1-1

LocationData structureType of testCI/powerP valueComparison
5B Normally distributedUnpaired t test10.63 to 87.290.0142EGFP vs Ubc9-OE
5E Normally distributedOne-way ANOVA0.8219<0.0001Ubc9 expression levels
Normally distributedTukey's post hoc–41.45 to –4.6250.012EGFP vs Ubc9-OE
Normally distributedTukey's post hoc12.86 to 49.680.0009EGFP vs Ubc9-RNAi
Normally distributedTukey's post hoc35.89 to 72.71<0.0001Ubc9-OE vs Ubc9-RNAi
Normally distributedTukey's post hoc–39.38 to –2.5600.0229Ubc9-RNAi vs NC1
5F Normally distributedOne-way ANOVA0.72410.0001α-Syn expression levels
Normally distributedTukey's post hoc–42.22 to –6.0780.025EGFP vs Ubc9-OE
Normally distributedTukey's post hoc4.374 to 40.510.0117EGFP vs Ubc9-RNAi
Normally distributedTukey's post hoc28.52 to 64.660.0001Ubc9-OE vs Ubc9-RNAi
Normally distributedTukey's post hoc–37.82 to –1.6820.029Ubc9-RNAi vs NC1
6B Normally distributedTwo-way ANOVA–35.37 to –27.83<0.0001Chx chase study at diff concentration
Normally distributedTukey's post hoc–41.70 to –17.930.0135Ubc9-OE 100 vs EGFP 100
Normally distributedTukey's post hoc–56.70 to –32.920.0001Ubc9-OE 150 vs EGFP 150
Normally distributedTukey's post hoc–63.67 to –39.89<0.0001Ubc9-OE 200 vs EGFP 200
6D Normally distributedTwo-way ANOVA–22.99 to –11.24<0.0001Chx chase study at diff time point
Normally distributedTukey's post hoc–52.30 to –8.9940.0008Ubc9-OE 18h vs EGFP 18h
Normally distributedTukey's post hoc–68.64 to –25.34<0.0001Ubc9-OE 24h vs EGFP 24h
7B Normally distributedTwo-way ANOVA–34.53 to –16.530.0002MG132-mediated proteasomal inhibition
Normally distributedTukey's post hoc25.60 to 86.88<0.0001EGFP Veh vs EGFP Chx
Normally distributedTukey's post hoc–88.91 to –25.970.0001EGFP Chx vs Ubc9-OE Chx
Normally distributedTukey's post hoc–68.03 to –5.0970.0343EGFP Chx vs EGFP Chx+MG132
Normally distributedTukey's post hoc–62.93 to –7.2990.0111EGFP Chx+MG132 vs Ubc9 Chx+MG132
8B Normally distributedTwo-way ANOVA–24.40 to –19.50<0.0001PMA chase study
Normally distributedTukey's post hoc–25.64 to –5.1570.0012EGFP 4μM vs Ubc9-OE 4μM
Normally distributedTukey's post hoc–41.26 to –20.770.0001EGFP 6μM vs Ubc9-OE 6μM
Normally distributedTukey's post hoc–46.18 to –25.69<0.0001EGFP 8μM vs Ubc9-OE 8μM
Normally distributedTukey's post hoc–59.03 to –38.55<0.0001EGFP 10μM vs Ubc9-OE μM
8D Normally distributedTwo-way ANOVA–17.12 to –1.0280.0009Effect of PMA on protein degradation
Normally distributedTukey's post hoc16.06 to 81.810.0002EGFP C vs EGFP PMA
Normally distributedTukey's post hoc–76.68 to –15.190.0002EGFP PMA vs Ubc9-OE PMA
Normally distributedTukey's post hoc–73.98 to –8.2390.0031EGFP PMA vs EGFP Cqn+PMA
9B Normally distributedTwo-way ANOVA0.81<0.0001SUMO1 level in total lysate
Normally distributedTukey's post hoc15.06 to 58.910.001EGFP vs EGFP MPP+
Normally distributedTukey's post hoc–43.90 to –0.059880.0493EGFP vs Ubc9
Normally distributedTukey's post hoc–69.67 to –25.83<0.0001EGFP MPP+ vs Ubc9 MPP+
9D Normally distributedTwo-way ANOVA0.81<0.0001SUMO1 level in α-syn IP'ed samples
Normally distributedTukey's post hoc7.692 to 65.010.0011EGFP vs EGFP MPP+
Normally distributedTukey's post hoc–62.93 to –5.6100.0166EGFP vs Ubc9
Normally distributedTukey's post hoc–99.03 to –41.72<0.0001EGFP MPP+ vs Ubc9 MPP+
9F Normally distributedTwo-way ANOVA0.620.0002Ubiquitin level in total lysate
Normally distributedTukey's post hoc1.485 to 57.870.0034EGFP vs EGFP MPP+
Normally distributedTukey's post hoc–52.79 to 3.5950.0369EGFP vs Ubc9
Normally distributedTukey's post hoc12.41 to 68.790.0036Ubc9 vs Ubc9 MPP+
9H Normally distributedTwo-way ANOVA0.72<0.0001Ubiquitin level in α-syn IP'ed samples
Normally distributedTukey's post hoc19.70 to 63.210.0081EGFP vs EGFP MPP+
Normally distributedTukey's post hoc–84.50 to –19.320.0013EGFP vs Ubc9
Normally distributedTukey's post hoc–74.35 to –9.1690.0092EGFP MPP+ vs Ubc9 MPP+
Normally distributedTukey's post hoc8.176 to 73.360.0111Ubc9 vs Ubc9 MPP+
1-1A Normally distributedOne-way ANOVA0.81<0.0001Effect of PFF on cell viability
Normally distributedDunnett's post hoc25.90 to 47.02<0.0001Vehicle vs PFF 1μg
Normally distributedDunnett's post hoc29.84 to 50.96<0.0001Vehicle vs PFF 2μg
Normally distributedDunnett's post hoc37.21 to 55.50<0.0001Vehicle vs PFF 3μg
Normally distributedDunnett's post hoc44.66 to 65.78<0.0001Vehicle vs PFF 4μg
Normally distributedDunnett's post hoc41.98 to 60.27<0.0001Vehicle vs PFF 5μg
1-1B Normally distributedOne-way ANOVA0.78<0.0001Effect of PFF on cytotoxicity
Normally distributedDunnett's post hoc–35.37 to –11.32<0.0001Vehicle vs PFF 1μg
Normally distributedDunnett's post hoc–37.83 to –13.78<0.0001Vehicle vs PFF 2μg
Normally distributedDunnett's post hoc–50.65 to –29.82<0.0001Vehicle vs PFF 3μg
Normally distributedDunnett's post hoc–63.02 to –38.97<0.0001Vehicle vs PFF 4μg
Normally distributedDunnett's post hoc–61.21 to –40.38<0.0001Vehicle vs PFF 5μg