Statistical analyses of MOR knock-down in D1-, D2-, A2a-, and ChAT-flMOR lines by RNA ISH and qPCR ( Fig. 1)
| Item | Figure | Experiment | Statistical test | Effect or interaction | Main effect | flMOR | D1flMOR | D2flMOR | A2aflMOR | ChATflMOR |
|---|---|---|---|---|---|---|---|---|---|---|
| a | 1Bi | RNA in situ hybridization | One-way ANOVA | Genotype, oprm1 and drd1 probes | F(3,16) = 29.14, p < 0.0001 | Reference genotype n = 5 | p < 0.0001 n = 5 | p = 0.02 n = 5 | N.S. n = 5 | N.A. |
| b | 1Bii | RNA in situ hybridization | One-way ANOVA | Genotype, oprm1 and drd2 probes | F(3,15) = 8.76, p = 0.0013 | Reference genotype n = 5 | p < 0.99 n = 5 | p = 0.004 n = 5 | N.S. n = 4 | N.A. |
| c | 1Bii | RNA in situ hybridization | One-way ANOVA | Genotype, oprm1, drd1 and ChAT probes | F(3,13) = 15.95, p = 0.0001 | Reference genotype n = 4 | N.A. | N.A. | N.A. | ChAT+ vs -; p < 0.0001, n = 4 D1+ vs -; NS n = 5 and 4 |
| d | 1Ci | qPCR | One-way ANOVA | Genotype, oprm1 probe | F(4,35) = 8.59, p < 0.0001 | Reference genotype n = 13 | p = 0.0002, n = 6 | p = 0.015, n = 8 | N.S., n = 6 | N.S., n = 7 |
| e | 1Cii | qPCR | One-way ANOVA | Genotype, drd1 probe | F(6,26) = 0.339, N.S. | Reference genotype n = 6 | N.S., n = 6 | N.S., n = 5 | N.S., n = 5 | N.S., n = 6 |
| f | 1Ciii | qPCR | One-way ANOVA | Genotype, drd2 probe | F(5,29) = 0.925, N.S. | Reference genotype n = 10 | N.S., n = 5 | N.S., n = 7 | N.S., n = 5 | N.S., n = 6 |
The RNA probes used were; oprm1 (MOR), drd1 (D1 receptor), drd2 (D2 receptor), and ChAT (cholineacetyltransferase) in flMOR, D1-, D2-, A2a-, and ChAT-flMOR lines. The qPCR probes used were oprm1 (MOR), drd1 (D1 receptor), drd2 (D2 receptor) in flMOR, D1-, D2-, A2a-, and ChAT-flMOR lines. RNA ISH: RNA in situ hybridization, N.S.: not significant, N.A: not applicable