Summary of statistical analyses
| Figures | Comparison | Statistical test | p value | F, Dfn, Dfd | |
|---|---|---|---|---|---|
| 1B | 1 d | 22°C vs 15°C | Two-way ANOVA, Turkey'smultiple comparison test | 0.0163 | Interaction: 15.96, 6, 18; time point:71.17, 3, 18; temperature: 142.8, 2, 6 |
| 22°C vs 10°C | 0.0019 | ||||
| 2 d | 22°C vs 15°C | 0.0001 | |||
| 22°C vs 10°C | 0.0001 | ||||
| 3 d | 22°C vs 15°C | 0.0001 | |||
| 22°C vs 10°C | 0.0001 | ||||
| 4 d | 22°C vs 15°C | 0.0001 | |||
| 22°C vs 10°C | 0.0001 | ||||
| 1C | HSP90α | 22°C vs 15°C | Two-way ANOVA, Turkey'smultiple comparison test | 0.5097 | Interaction: 11.22, 6, 24; Gene: 38.81,3, 24; temperature: 43.31, 2, 24 |
| 22°C vs 10°C | 0.159 | ||||
| 15°C vs 10°C | 0.0155 | ||||
| HSP90β | 22°C vs 15°C | 0.0489 | |||
| 22°C vs 10°C | 0.0024 | ||||
| 15°C vs 10°C | 0.4152 | ||||
| Grp94 | 22°C vs 15°C | <0.0001 | |||
| 22°C vs 10°C | <0.0001 | ||||
| 15°C vs 10°C | 0.0012 | ||||
| TRAP-1 | 22°C vs 15°C | 0.2602 | |||
| 22°C vs 10°C | 0.1889 | ||||
| 15°C vs 10°C | 0.9793 | ||||
| 1E | HSP90β | 22°C vs 10°C | Unpaired t test with Welch'scorrection | 0.7421 | 4.39, 2, 2 |
| Grp94 | 22°C vs 10°C | 0.0035 | 3.03, 2, 2 | ||
| 1F | Control vs 0.25 nm | One-way ANOVA, Turkey'smultiple comparison test | 0.0067 | 75.47, 3, 12 | |
| Control vs 0.5 nm | <0.0001 | ||||
| Control vs 1 nm | <0.0001 | ||||
| 1H | Normalized AChR intensity cluster region | Control vs 17-AAG | Unpaired t test | 0.0403 | 4.762, 2, 2 |
| AChR-poor perforations/AChR cluster area | Unpaired t test | 0.02 | 2.462, 3, 3 | ||
| 1K | 24 h | Control vs 17-AAG | Two-way ANOVA, Sidak'smultiple comparison test | 0.0274 | Interaction: 0.02911, 1, 85; treatment:151.1, 1, 85; time point: 6.856, 1, 85 |
| 48 h | 0.0385 | ||||
| 1L | 24 h | Control vs 17-AAG | Two-way ANOVA, Sidak'smultiple comparison test | 0.9726 | Interaction: 0.5439, 1, 86; treatment:80.21, 1, 86; time point: 6.856, 1, 85 |
| 48 h | 0.4136 | ||||
| 1-1B | Polar metabolites | Control vs 17-AAG | Unpaired t test | Listed in ExtendedData Table 1-1 | N/A |
| 1-1D | Fatty acids | Unpaired t test | Listed in ExtendedData Table 1-2 | N/A | |
| 1-1F | Control vs CHX | One-way ANOVA, Dunnett'smultiple comparison test | 0.0142 | 9.046, 2, 6 | |
| Control vs 17-AAG | 0.8498 | ||||
| 1-1H | Control vs 17-AAG | Unpaired t test with Welch'scorrection | 0.3747 | 1.388, 32, 41 | |
| 2B | 1 d, Without photobleaching:Control vs 1 d, Without photobleaching:17-AAG | Two-way ANOVA, Turkey'smultiple comparison test | 0.0198 | Interaction: 6.99, 3, 6; treatment:6.916, 1, 2; time point: 10.88, 3, 6 | |
| 1 d, Without photobleaching:Control vs 3 d, Without photobleaching:Control | 0.6093 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Without photobleaching:17-AAG | 0.0318 | ||||
| 1 d, Without photobleaching:Control vs 1 d, Photobleachingof aneural AChR cluster:Control | 0.0069 | ||||
| 1 d, Without photobleachingControl vs 1 d, Photobleachingof aneural AChR cluster:17-AAG | 0.004 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Photobleachingof aneural AChR cluster:Control | 0.0011 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.0018 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Without photobleaching:Control | 0.126 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Without photobleaching:17-AAG | 0.9991 | ||||
| 1 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:Control | 0.8927 | ||||
| 1 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.5569 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.0818 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.1828 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Without photobleaching:17-AAG | 0.2166 | ||||
| 3 d, Without photobleaching:Control vs 1 d, Photobleaching of aneuralAChR cluster:Control | 0.0356 | ||||
| 3 d, Without photobleaching:Control vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.018 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.004 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.007 | ||||
| 3 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:Control | 0.6672 | ||||
| 3 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.3446 | ||||
| 3 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.0487 | ||||
| 3 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.1064 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.9916 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.3032 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.6183 | ||||
| 1 d, Photobleaching of aneural AChR cluster:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.6054 | ||||
| 1 d, Photobleaching of aneural AChR cluster:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.9315 | ||||
| 3 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.9898 | ||||
| 2C | 1 d, Without photobleaching:Control vs 1 d, Without photobleaching:17-AAG | Two-way ANOVA, Turkey'smultiple comparison test | >0.9999 | Interaction: 0.293, 3, 6; treatment:0.2994, 1, 2; time point: 11.74, 3, 6 | |
| 1 d, Without photobleaching:Control vs 3 d, Without photobleaching:Control | 0.406 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Without photobleaching:17-AAG | 0.6936 | ||||
| 1 d, Without photobleaching:Control vs 1 d, Photobleaching of aneural AChRcluster:Control | >0.9999 | ||||
| 1 d, Without photobleaching:Control vs 1 d, Photobleaching of aneural AChRcluster:17-AAG | >0.9999 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChRcluster:Control | 0.832 | ||||
| 1 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.9999 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Without photobleaching:Control | 0.4594 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Without photobleaching:17-AAG | 0.7563 | ||||
| 1 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChRcluster:Control | >0.9999 | ||||
| 1 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | >0.9999 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.8826 | ||||
| 1 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | >0.9999 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Without photobleaching:17-AAG | 0.9968 | ||||
| 3 d, Without photobleaching:Control vs 1 d, Photobleaching of aneural AChRcluster:Control | 0.4322 | ||||
| 3 d, Without photobleaching:Control vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.4267 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.9722 | ||||
| 3 d, Without photobleaching:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.5671 | ||||
| 3 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:Control | 0.7253 | ||||
| 3 d, Without photobleaching:17-AAG vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | 0.7188 | ||||
| 3 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | >0.9999 | ||||
| 3 d, Without photobleaching:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.8595 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 1 d, Photobleaching of aneural AChR cluster:17-AAG | >0.9999 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.8583 | ||||
| 1 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | >0.9999 | ||||
| 1 d, Photobleaching of aneural AChR cluster:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:Control | 0.853 | ||||
| 1 d, Photobleaching of aneural AChR cluster:17-AAG vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | >0.9999 | ||||
| 3 d, Photobleaching of aneural AChR cluster:Control vs 3 d, Photobleaching of aneural AChR cluster:17-AAG | 0.9511 | ||||
| 3B | WT vs Control MO | One-way ANOVA, Dunnett'smultiple comparison test | 0.1383 | 21.35, 2, | |
| WT vs Grp94 MO | 0.0089 | ||||
| 3D | Top | WT vs Control MO | Two-way ANOVA, Turkey'smultiple comparison test | 0.7282 | Interaction: 4.283, 4, 18; treatment:127.9, 2, 18; Cluster type:80.22, 2, 18 |
| WT vs Grp94 MO | 0.0002 | ||||
| Control MO vs Grp94 MO | 0.0008 | ||||
| Bottom | WT vs Control MO | 0.1487 | |||
| WT vs Grp94 MO | 0.0013 | ||||
| Control MO vs Grp94 MO | <0.0001 | ||||
| Total | WT vs Control MO | 0.974 | |||
| WT vs Grp94 MO | <0.0001 | ||||
| Control MO vs Grp94 MO | <0.0001 | ||||
| 3E | Normalized intensity of AChRat cluster region | WT vs Control MO | One-way ANOVA, Dunnett'smultiple comparison test | 0.7416 | 6.557, 2, 9 |
| WT vs Grp94 MO | 0.0141 | ||||
| Ratio of perforated area/entireAChR cluster area | WT vs Control MO | 0.8166 | 31.46, 2, 9 | ||
| WT vs Grp94 MO | 0.0002 | ||||
| 3G | GFP-XAC vs GFP-XAC + Control MO | Two-way ANOVA, Sidak'smultiple comparison test | 0.6801 | Interaction: 6.469, 2, 45; treatment:80.39, 1, 45; time point: 6.469, 2, 45 | |
| GFP-XAC vs GFP-XAC + Grp94 MO | 0.0162 | ||||
| 3I | Normalized intensity of AChR at bead contacts | GFP-XAC alone vs GFP-XAC + Control MO | One-way ANOVA, Turkey'smultiple comparison test | 0.8926 | 6.716, 2, 9 |
| GFP-XAC alone vs GFP-XAC + Grp94 MO | 0.0201 | ||||
| GFP-XAC + Control MO vs GFP-XAC + Grp94 MO | 0.0409 | ||||
| Ratio of GFP-XAC intensity at bead/non-bead contact region | GFP-XAC alone vs GFP-XAC + Control MO | 0.3488 | 4.59, 2, 9 | ||
| GFP-XAC alone vs GFP-XAC + Grp94 MO | 0.0345 | ||||
| GFP-XAC + Control MO vs GFP-XAC + Grp94 MO | 0.3109 | ||||
| 3H | 0 h | GFP-XAC alone vs Control MO + GFP-XAC | Two-way ANOVA, Sidak'smultiple comparison test | 0.9862 | Interaction: 2.583, 2, 29; treatment:18.65, 1, 29; time point: 4.2, 2, 29 |
| GFP-XAC alone vs Grp94 MO + GFP-XAC | 0.0038 | ||||
| 4 h | GFP-XAC alone vs Control MO + GFP-XAC | 0.7729 | |||
| GFP-XAC alone vs Grp94 MO + GFP-XAC | 0.9996 | ||||
| GFP-XAC alone | 0 h vs 4 h | 0.0012 | |||
| Control MO + GFP-XAC | 0.0299 | ||||
| Grp94 MO + GFP-XAC | 0.8305 | ||||
| 3-1B | XAC | WT vs Control MO | One-way ANOVA, Dunnett'smultiple comparison test | 0.1955 | 12.16, 2, 9 |
| WT vs Grp94 MO | 0.0016 | ||||
| 3-1C | p34-Arc | WT vs Control MO | 0.9667 | 7.445, 2, 6 | |
| WT vs Grp94 MO | 0.0242 | ||||
| 3-1D | Vinculin | WT vs Control MO | 0.8551 | 5.924, 2, 6 | |
| WT vs Grp94 MO | 0.0331 | ||||
| 4C | Perforated region | Control vs 17-AAG | Unpaired t test with Welch'scorrection | 0.0025 | 11.1, 11, 5 |
| AChR-rich region | Control vs 17-AAG | 0.049 | 11.64, 10, 5 | ||
| 4-1C | Perforated region | GFP-XAC vs GFP-XAC (S3A) | One-way ANOVA, Turkey'smultiple comparison test | 0.1195 | 16.04, 2, 27 |
| GFP-XAC vs GFP-XAC (S3E) | 0.0015 | ||||
| GFP-XAC (S3A) vs GFP-XAC (S3E) | <0.0001 | ||||
| AChR-rich region | GFP-XAC vs GFP-XAC (S3A) | 0.0025 | 16.9, 2, 27 | ||
| GFP-XAC vs GFP-XAC (S3E) | 0.0497 | ||||
| GFP-XAC (S3A) vs GFP-XAC (S3E) | <0.0001 | ||||
| 5B | WT vs Control MO | One-way ANOVA, Turkey'smultiple comparison test | 0.511 | 44.59, 2, 6 | |
| WT vs Grp94 MO | 0.0003 | ||||
| Control MO vs Grp94 MO | 0.0007 | ||||
| 5C | WT vs Control MO | One-way ANOVA, Turkey'smultiple comparison test | 0.9022 | 8.63, 2, 6 | |
| WT vs Grp94 MO | 0.0351 | ||||
| Control MO vs Grp94 MO | 0.0211 | ||||
| 5F | WT vs Control MO | Kruskal–Wallis ANOVA testwith Dunn's multiplecomparison test | N/A | N/A | |
| WT vs Grp94 MO | |||||
| Control MO vs Grp94 MO | |||||
| 5G | WT vs Control MO | Kruskal–Wallis ANOVA testwith Dunn's multiplecomparison test | |||
| WT vs Grp94 MO | |||||
| Control MO vs Grp94 MO | |||||
| 5-1B | Control vs 17-AAG | One-way ANOVA, Dunnett'smultiple comparison test | 0.0009 | 13.85, 4, 11 | |
| Control vs PU-WS13 | 0.0074 | ||||
| Control vs Control MO | 0.8996 | ||||
| Control vs Grp94 MO | 0.0004 | ||||
| 5-1C | Control vs 17-AAG | 0.0195 | 5.745, 4, 11 | ||
| Control vs PU-WS13 | 0.0233 | ||||
| Control vs Control MO | 0.9971 | ||||
| Control vs Grp94 MO | 0.0329 | ||||
| 5-2B | 0.5 h | Control vs 17-AAG | One-way ANOVA, Dunnett'smultiple comparison test | 0.0001 | 9.548, 2, 74 |
| Control vs PU-WS13 | 0.0154 | ||||
| 4 h | Control vs 17-AAG | 0.0001 | 20.28, 2, 73 | ||
| Control vs PU-WS13 | 0.0001 | ||||