Key resources table
| Reagent or resource | Source | Identifier | RRID |
|---|---|---|---|
| Experimental models: organisms/strains | |||
| Cortical neuron cultures from P0 C57BL/6J | The Jackson Laboratory | Catalog #JAX:000664 | RRID:IMSR_JAX:000664 |
| Cortical neuron cultures from P0 Panx1 KO on a C57BL/6J background | (Dvoriantchikova et al., 2012;Sanchez-Arias et al., 2019) | NA | NA |
| Recombinant DNA | |||
| mCherry-CD9-10 | Addgene | Plasmid #55013 | RRID:Addgene_55013 |
| pEGFP-N1 | Clontech (Takara Bio), discontinued | Catalog #6085-1 | NA |
| Panx1-EGFP | (Penuela et al., 2007) | NA | NA |
| Chemicals, recombinant proteins | |||
| DMEM/F12 | Thermo Fisher Scientific | NA | |
| NeuroCult | STEMCELL Tech. | Catalog #05713 | NA |
| BrainPhys | STEMCELL Tech. | Catalog #05790 | NA |
| Neurocult SM1 | STEMCELL Tech. | Catalog #05711 | NA |
| GlutaMAX | Thermo Fisher Scientific | Catalog #35050061 | NA |
| P/S | Thermo Fisher Scientific | Catalog #15140122 | NA |
| Gentamicin | MilliporeSigma | G1397 | NA |
| Poly-D-lysine hydrobromide (PDL) | MilliporeSigma | P6407 | NA |
| Dispase-1 | MilliporeSigma | D4818-2MG | NA |
| Papain | MilliporeSigma | P4762-25MG | NA |
| DNAse-1 | MilliporeSigma | 11284932001 | NA |
| Cytosine β-D-arabinofuranoside (ara-C) | MilliporeSigma | C1768 | NA |
| Lipofectamine2000 | Thermo Fisher Scientific | Catalog #11668027 | NA |
| OptiMEM I | Thermo Fisher Scientific | Catalog #31985062 | NA |
| Probenecid (water-soluble) | Thermo Fisher Scientific | Catalog #P36400 | NA |
| My-Taq Extract PCR kit Vectashield | Bioline Vector Laboratories | BIO-21126 H-1000 | NA RRID:AB_2336789 |
| Software and algorithms | |||
| FIJI (FIJI is just ImageJ) | NIH (Schindelin et al., 2012) | NA | RRID:SCR_002285 |
| MultiStackReg v1.45 R Project for Statistical Computing (version 3.6.2) | Brad Busse (http://bradbusse.net/MultiStackReg1.45_.jar) The R Foundation | NA NA | NA RRID:SCR_001905 |
| Rstudio | Rstudio Inc. | NA | RRID:SCR_000432 |
| tidyverse package for R | CRAN (Grolemund and Wickham,2017) | NA | RRID:SCR_014601 |
| dabestr package for R | CRAN (Ho et al., 2019) | NA | NA |
| Adobe Photoshop CS6 Leica Application Suite Software version 3.1.3.16308 | Adobe Systems Inc. Leica Microsystems GmbH | NA NA | RRID:SCR_014199 RRID:SCR_013673 |
| Equipment | |||
| Leica TCS SP8 | Leica Microsystems GmbH | NA | NA |
| 8-well Nunc Lab-Tek Chamberedcoverglass PDL precoated coverslips | Thermo Fisher Scientific NeuVitro | 155411PKGG- 12-PDL | NA NA |