Table 1

Summary of statistical analyses

						Estimation statistics
Figure	Measurement	Datastructure	Type of test	Comparison	Statisticalvalue	Two-sidedpermutationt test value	Mean DifferenceWith 95% ConfidenceInterval
Fig. 1D	Maximum migration speed	Normal	Unpaired t test	Control vs SP	p = 1.68E-10; t₍₂₀₎ = 11.86	0.0	–54.2 [–63.1, –46.0]
Fig. 1D	Mean migration speed	Normal	Unpaired t test	Control vs SP	p = 1.68E-09; t₍₂₀₎ = 10.38	0.0	–28.1 [–33.8, –23.6]
Fig. 1D	Minimum migration speed	Normal	Unpaired t test	Control vs SP	p = 0.0000717; t₍₂₀₎ = 4.98	0.0	–4.68 [–6.76, –3.08]
Fig. 1E	Speed variation	Normal	Unpaired t test	Control vs SP	p = 0.000188; t₍₂₀₎ = –4.56	0.0002	0.136 [0.0827, 0.196]
Fig. 1F	Displacement	Normal	Unpaired t test	Control vs SP	p = 4.73E-07; t₍₂₀₎ = 7.29	0.0	–81.2 [–1.06e+02, –63.6]
Fig. 1G	Straightness	Normal	Unpaired t test	Control vs SP	p = 0.451; t₍₂₀₎ = 0.769	0.389	–0.0282 [–0.0955, 0.03]
Fig. 2C	Maximum leadingprocess length	Normal	Unpaired t test	Control vs SP	p = 0.977; t₍₁₈₎ = –0.0294	0.975	0.177 [–10.7, 11.7]
Fig. 2C	Mean leading processlength	Normal	Unpaired t test	Control vs SP	p = 0.947; t₍₁₈₎ = 0.0668	0.947	–0.257 [–6.85, 7.48]
Fig. 2C	Minimum leadingprocess length	Normal	Unpaired t test	Control vs SP	p = 0.911; t₍₁₈₎ = –0.112	0.917	0.413 [–6.12, 7.32]
Fig. 2D	Frequency of growthcone splits	Normal	Unpaired t test	Control vs SP	p = 0.0169; t₍₃₄₎ = 2.51	0.0146	–0.681 [–1.13, –0.121]
Fig. 2G	Frequency of side branches	Normal	Unpaired t test	Control vs SP	p = 0.900; t₍₃₄₎ = 0.126	0.897	0.0347 [–0.479, 0.499]
Fig. 2H	Side branch duration	Normal	Unpaired t test	Control vs SP	p = 0.016; t₍₁₀₂₎ = 2.46	0.0124	–7.58 [–14.2, –2.12]
Fig. 3C	Nucleokinesis distance	Normal	Unpaired t test	Control vs SP	p = 2.36E-10; t₍₁₈₎ = 12.58	0.0	–6.36 [–7.3, –5.42]
Fig. 3D	Nucleokinesis frequency	Normal	Unpaired t test	Control vs SP	p = 1.92E-08; t₍₁₈₎ = 8.96	0.0	–0.765 [–0.912, –0.598]
Fig. 3E	Pause duration	Normal	Unpaired t test	Control vs SP	p = 1.45E-07; t₍₁₈₎ = –7.89	0.0	9.5 [7.44, 11.8]
Fig. 3F	Soma-swelling distance	Normal	Unpaired t test	Control vs SP	p = 0.001698; t₍₁₈₎ = 3.68	0.0022	–1.79 [–2.64, –0.824]
Fig. 3G	Swelling duration	Normal	Unpaired t test	Control vs SP	p = 0.00047; t₍₁₄₎ = –4.53	0.0014	7.04 [4.33, 9.76]
Fig. 4D	Maximum migration speed	Normal	Unpaired t test	WT vs cTKO	p = 0.981; t₍₂₃₎ = 0.0236	0.982	–0.117 [–9.81, 9.2]
Fig. 4D	Mean migration speed	Normal	Unpaired t test	WT vs cTKO	p = 0.105; t₍₂₃₎ = 1.69	0.11	–5.59 [–12.0, 0.867]
Fig. 4D	Minimum migration speed	Normal	Unpaired t test	WT vs cTKO	p = 0.260; t₍₂₃₎ = 1.16	0.256	–1.24 [–3.27, 0.771]
Fig. 4E	Speed variation	Normal	Unpaired t test	WT vs cTKO	p = 0.022; t₍₂₃₎ = –2.46	0.0208	0.057 [0.0166, 0.107]
Fig. 4F	Displacement	Normal	Unpaired t test	WT vs cTKO	p = 0.048; t₍₂₃₎ = 2.09	0.0492	–29.1 [–54.8, –0.875]
Fig. 4G	Straightness	Normal	Unpaired t test	WT vs cTKO	p = 0.027; t₍₂₃₎ = 2.37	0.0284	–0.0651 [–0.118, –0.0133]
Fig. 4H	Frequency of growthcone splits	Normal	Unpaired t test	WT vs cTKO	p = 0.0454; t₍₂₀₎ = 2.13	0.0318	–0.618 [–1.29, –0.183]
Fig. 4I	Frequency of sidebranches	Normal	Unpaired t test	WT vs cTKO	p = 0.658; t₍₂₀₎ = 0.448	0.654	–0.113 [–0.542, 0.385]
Fig. 4J	Side branch duration	Normal	Unpaired t test	WT vs cTKO	p = 0.046; t₍₆₀₎ = 2.04	0.044	–8.83 [–17.7, –1.83]
Fig. 5C	Nucleokinesis distance	Normal	Unpaired t test	WT vs cTKO	p = 0.028; t₍₁₈₎ = 2.39	0.0298	–0.92 [–1.61, –0.17]
Fig. 5D	Nucleokinesis frequency	Normal	Unpaired t test	WT vs cTKO	p = 0.00203; t₍₁₈₎ = –3.60	0.0012	0.458 [0.262, 0.752]
Fig. 5E	Pause duration	Normal	Unpaired t test	WT vs cTKO	p = 0.000464; t₍₁₈₎ = 4.27	0.0002	–5.09 [–7.84, –3.27]
Fig. 5F	Swelling duration	Normal	Unpaired t test	WT vs cTKO	p = 0.00257; t₍₁₀₎ = 3.99	0.0012	–2.61 [–3.94, –1.54]
Fig. 6D	Centrosome localization	Normal	Two-way ANOVA(post hoc Fisher’sexact test)	Control vs SP	p < 0.0001; F_(2,114) = 13.82	NA	NA
Fig. 6E	Centrosome presencein swelling	Normal	χ²	Control vs SP	p < 0.0001; Χ²_{(331.1, 1)} =18.20	NA	NA
Fig. 6F	Centrosome maximumdistance forward	Normal	Unpaired t test	Control vs SP	p = 0.028; t₍₃₈₎ = 2.30	0.0256	–3.2 [–5.82, –0.79]
Fig. 6F	Centrosome maximumdistance behind	Normal	Unpaired t test	Control vs SP	p = 0.000015; t₍₃₈₎ = 4.97	0	–10.3 [–14.6, –6.49]
Fig. 7C	Cilia localization	Normal	Two-way ANOVA(post hoc Fisher'sexact test)	Control vs SP	p < 0.0001; F_(2,114) = 12.13	NA	NA
Fig. 7D	Cilia presence in swelling	Normal	χ²	Control vs SP	p < 0.0001; Χ²_(314.2,1) = 17.72	NA	NA
Fig. 7E	Cilia maximum distanceforward	Normal	Unpaired t test	Control vs SP	p = 0.0094; t₍₃₈₎ = 2.76	0.0094	–4.16 [–7.0, –1.61]
Fig. 7E	Cilia maximum distance behind	Normal	Unpaired t test	Control vs SP	p = 0.017; t₍₃₈₎ = 2.51	0.0152	–6.38 [–11.6, –1.97]
Fig. 7F	Cilia length	Normal	Unpaired t test	Control vs SP	p = 0.558; t₍₃₀₎ = 0.592	0.575	–0.0983 [–0.439, 0.195]
Extended Data Fig. 1-1A	Mean migration speed all cells	Normal	Unpaired t test	Control vs SP	p = 4.65E-53; t₍₂₅₂₎ = 19.74	0.0	–28.4 [–31.1, –25.5]
Extended Data Fig. 1-1B	Maximum migration speed	Normal	Unpaired t test	Control vs SP	p = 0.0053; t₍₂₅₎ = 3.05	0.0042	–24.0 [–41.8, –10.6]
Extended Data Fig. 1-1B	Mean migration speed	Normal	Unpaired t test	Control vs SP	p = 0.559; t₍₂₅₎ = 0.592	0.545	–0.299 [–1.28, 0.636]
Extended Data Fig. 1-1B	Minimum migration speed	Normal	Unpaired t test	Control vs SP	p = 0.209; t₍₂₅₎ = –1.29	0.210	1.39 [–0.708, 3.25]
Extended Data Fig. 1-1C	Speed variation	Normal	Unpaired t test	Control vs SP	p = 0.0038; t₍₂₅₎ = 3.19	0.0028	–0.187 [–0.317, –0.0848]
Extended Data Fig. 1-1D	Displacement	Normal	Unpaired t test	Control vs SP	p = 0.158; t₍₂₅₎ = –1.45	0.172	15.6 [–5.9, 35.7]
Extended Data Fig. 1-1E	Straightness	Normal	Unpaired t test	Control vs SP	p = 0.146; t₍₂₅₎ = –1.49	0.162	0.104 [–0.0304, 0.238]
Extended Data Fig. 1-1F	Soma-swelling distance	Normal	Unpaired t test	Control vs SP	p = 0.0017; t₍₂₅₎ = 3.51	0.0008	–2.3 [–3.7, –1.19]
Extended Data Fig. 1-1G	Average nucleokinesis distance	Normal	Unpaired t test	Control vs SP	p = 8.26E-06; t₍₂₅₎ = 5.58	0.0	–3.94 [–5.36, –2.6]
Extended Data Fig. 1-1H	<10-µm nucleokinesis distance	Normal	Unpaired t test	Control vs SP	p = 0.239; t₍₂₅₎ = –1.20	0.236	0.259 [–0.113, 0.719]
Extended Data Fig. 1-1I	>10-µm nucleokinesis distance	Normal	Unpaired t test	Control vs SP	p = 0.00094; t₍₂₅₎ = 3.75	0.0004	–2.73 [–4.26, –1.43]
Extended Data Fig. 1-1J	Nucleokinesis frequency	Normal	Unpaired t test	Control vs SP	p = 0.382; t₍₂₅₎ = 0.890	0.381	–0.111 [–0.345, 0.123]
Extended Data Fig. 1-1K	Pause duration	Normal	Unpaired t test	Control vs SP	p = 0.805; t₍₂₅₎ = –0.250	0.805	0.363 [–2.62, 2.85]
Extended Data Fig. 1-1L	>10-µm nucleokinesis frequency	Normal	Unpaired t test	Control vs SP	p = 0.087; t₍₂₅₎ = 1.78	0.098	–0.233 [–0.458, 0.0489]
Extended Data Fig. 1-1M	Frequency of growth cone splits	Normal	Unpaired t test	Control vs SP	p = 0.00398; t₍₈₎ = 3.99	0.0096	–1.33 [–1.81, –0.612]
Extended Data Fig. 1-1N	Frequency of side branches	Normal	Unpaired t test	Control vs SP	p = 0.324; t₍₈₎ = 1.05	0.329	–0.44 [–1.1, 0.369]
Extended Data Fig. 1-1O	Side branch duration	Normal	Unpaired t test	Control vs SP	p = 0.529; t₍₂₅₎ = 0.639	0.556	–3.16 [–14.8, 4.59]

All statistical measurements performed in the study, organized by figure panel. The type of measurement, data structure, type of test, group comparison, and statistical values are included for each analysis. Estimation statistics are provided, where applicable. SP = SP600125.