Table 1

Results of two-way repeated measures ANOVA of song quality measures

Song measureFactordfFpEstimation statistics
difference (muscimol – saline)
Number of songsCannula placement
Treatment interaction		2
1
2		0.328
0.005
1.855		0.728
0.944
0.206		ICo
Miss
PAG		8.5
–23.4
25.2		95 CI [–6.67; 22.3]
95 CI [–9.83; 140]
95 CI [–9.83; 140]
Time spentsingingCannula placement
Treatment interaction		2
1
2		0.574
0.130
1.359		0.581
0.726
0.301		ICo
Miss
PAG		24.6
–117
230		95 CI [–58.9; 89.3]
95 CI [–229; –20.2]
95 CI [–56.5; 1240]
Average songdurationCannula placement
Treatment interaction		2
1
2		0.662
0.164
0.626		0.537
0.694
0.554		ICo
Miss
PAG		–0.88
0.089
0.256		95 CI [–3.34; 1.35]
95 CI [–1.06; 1.55]
95 CI [–1.56; 1.77]
Average intersongintervalCannula placement
Treatment interaction		2
1
2		0.757
0.0
0.2		0.494
0.985
0.822		ICo
Miss
PAG		–13.6
9.61
39		95 CI [–153; 43.4]
95 CI [–147; 166]
95 CI [–180; 203]
Average songelementsCannula placement
Treatment interaction		2
1
2		0.747
0.407
0.354		0.499
0.538
0.710		ICo
Miss
PAG		1
–0.661
1.3		95 CI [–1.78; 6.86]
95 CI [–14; 14]
95 CI [–1.89; 6.2]
Average energyCannula placement20.6070.564ICo0.000295 CI [0.00004; 0.0006]
Treatment interaction10.0010.989Miss–0.00000895 CI [–0.0009; 0.0001]
22.5410.128PAG–0.000395 CI [–0.0009; 0.0001]
Average peak topeak amplitudeCannula placement
Treatment interaction		2
1
2		1.12
0.753
2.616		0.372
0.411
0.134		ICo
Miss
PAG		0.014
0.003
–0.037		95 CI [0.001; 0.038]
95 CI [–0.014; 0.026]
95 CI [–0.083; 0.008]
Average RMSCannula placement
Treatment interaction		2
1
2		0.485
0.312
4.828		0.629
0.589
0.034*		ICo
Miss
PAG		0.528
0.796
–2.43		95 CI [–0.002; 1.52]
95 CI [–0.942; 2.31]
95 CI [–5.19; 0.123]
Average entropyCannula placement20.0070.993ICo0.000995 CI [–0.010; 0.008]
Treatment interaction11.6830.224Miss–0.000995 CI [–0.007; 0.002]
20.1710.845PAG0.000495 CI [–0.014; 0.012]
Max. fundamentalfrequencyCannula placement
Treatment interaction		2
1
2		0.385
1.274
0.014		0.690
0.285
0.986		ICo
Miss
PAG		–52.4
–80
–44.3		95 CI [–363; 225]
95 CI [–221; 85.2]
95 CI [–613; 614]
Max. bandwidthCannula placement
Treatment interaction		2
1
2		0.343
0.084
0.747		0.718
0.778
0.498		ICo
Miss
PAG		110
–43.4
–79.4		95 CI [–129; 495]
95 CI [–286; 37]
95 CI [–348; 271]
Max. peakamplitudeCannula placement
Treatment interaction		2
1
2		0.641
0.512
2.214		0.547
0.491
0.160		ICo
Miss
PAG		0.332
0.15
–2.88		95 CI [–1.94; 2.43]
95 CI [–3.64; 2.84]
95 CI [–6.2; –0.045]
Max. peakfrequencyCannula placement
Treatment interaction		2
1
2		1.033
1.184
0.236		0.391
0.302
0.794		ICo
Miss
PAG		7.83
–182
–148		95 CI [–217; 149]
95 CI [–749; 45.9]
95 CI [–730; 492]