Table 1

Putative miR-92a target genes

Gene nameFlybase IDRNAi line PI for nSyb-GAL4 screenRetest scorep value
CG12024CG12024GD-201430.45 ± 0.02
CG8360CG8360GD-416430.40 ± 0.08  
cpr50CaCG13338KK-1003170.64 ± 0.070.56 ± 0.080.15
khc-73CG8183KK-1059840.38 ± 0.090.28 ± 0.07 0.02*
crebACG7450KK-1106500.37 ± 0.060.37 ± 0.080.09
  • Candidate mRNA targets for miR-92a were selected by applying the pipeline described in Figure 5A. Five genes were screened for a role in memory formation using an RNAi approach. The five lines from the Vienna Drosophila RNAi left ( that were tested are listed. RNAi lines were crossed to the nSyb-GAL4 driver and tested for 3-h memory with n = 4. Each individual RNAi line was compared with a daily nSyb-GAL4>UAS-dcr-2 control in the respective GD or KK background. The average performance index for the nSyb-GAL4>UAS-dcr-2 control was 0.36 ± 0.1 for the GD control (n = 4) and 0.49 ± 0.04 for the KK control. Three lines with a trend for a significant effect on memory were retested with n = 6. Only the khc-73 RNAi line, had a PI significantly lower than the control (n = 10). Results shown are the mean ± SEM. Two-tailed, two-sample Student’s t tests, *p < 0.05.