Summary of high-resolution respirometry data
| Striatum | ||||||
| State | LEAK | Oxidativephosphorylation (OXPHOS) | Uncoupled | |||
| Titration | Mal, Glu, Pyr | ADP | Succinate | CCCP | Rotenone | |
| Label | LEAK (NL) | NP | NSP | NSE | SE | |
| Respiration perwet weight [pmol O2/(s*mg)] | Veh PBS | 17.1 (2.0) | 73.4 (12.0) | 116.0 (15.0) | 140.7 (16.9) | 45.3 (5.4) |
| CORT PBS | 17.1 (2.5) | 72.5 (12) | 111.5 (16.1) | 137.0 (22.2) | 41.8 (3.9) | |
| Veh PFF | 17.0 (1.6) | 72.4 (14.0) | 112.9 (18.1) | 134.8 (20.6) | 45.7 (4.7) | |
| CORT PFF | 18.9 (2.9) | 80,8 (14.4) | 121.4 (19.0) | 148.9 (22.7) | 44.8 (8.5) | |
| Interaction | F(1,30) = 1.395 | F(1,30) = 1.055 | F(1,30) = 1.225 | F(1,30) = 1.589 | F(1,30) = 0.384 | |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||
| CORT factor | F(1,30) = 1.410 | F(1,30) = 0.711 | F(1,30) = 0.116 | F(1,30) = 0.544 | F(1,30) = 1.176 | |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||
| PFF factor | F(1,30) = 1.049 | F(1,30) = 0.655 | F(1,30) = 0.341 | F(1,30) = 0.181 | F(1,30) = 0.725 | |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||
| FCRs (normalizationto NSE) | Veh PBS | 0.12 (0.01) | 0.52 (0.03) | 0.82 (0.02) | 1 | 0.32 (0.02) |
| CORT PBS | 0.13 (0.01) | 0.53 (0.03) | 0.82 (0.03) | 1 | 0.31 (0.03) | |
| Veh PFF | 0.13 (0.02) | 0.53 (0.03) | 0.84 (0.03) | 1 | 0.34 (0.03) | |
| CORT PFF | 0.13 (0.02) | 0.54 (0.03) | 0.82 (0.04) | 1 | 0.30 (0.04) | |
| Interaction | F(1,30) = 0.142 | F(1,30) = 0.044 | F(1,30) = 0.562 | - | F(1,30) = 1.902 | |
| n.s. | n.s. | n.s. | - | n.s. | ||
| CORT factor | F(1,30) = 0.050 | F(1,30) = 0.622 | F(1,30) = 2.244 | - | F(1,30) = 6.469 | |
| n.s. | n.s. | n.s. | - | * | ||
| PFF factor | F(1,30) = 0.622 | F(1,30) = 1.400 | F(1,30) = 0.395 | - | F(1,30) = 0.339 | |
| n.s. | n.s. | n.s. | - | n.s. | ||
| Mitochondrial ROS [pmol/(s*mgwet weight)] | Veh PBS | 0.11 (0.04) | 0.06 (0.02) | - | - | 0.39 (0.22) |
| CORT PBS | 0.12 (0.03) | 0.06 (0.02) | - | - | 0.37 (0.14) | |
| Veh PFF | 0.10 (0.04) | 0.07 (0.03) | - | - | 0.36 (0.13) | |
| CORT PFF | 0.14 (0.03) | 0.09 (0.02) | - | - | 0.35 (0.15) | |
| Interaction | F(1,29) = 0.733 | F(1,28) = 0.784 | - | - | F(1,29) = 0.010 | |
| n.s. | n.s. | - | - | n.s. | ||
| CORT factor | F(1,29) = 3.792 | F(1,28) = 0.848 | - | - | F(1,29) = 0.089 | |
| n.s. | n.s. | - | - | n.s. | ||
| PFF factor | F(1,29) = 0.490 | F(1,28) = 2.699 | - | - | F(1,29) = 0.275 | |
| n.s. | n.s. | - | - | n.s. | ||
| Amygdala | ||||||
| State | LEAK | Oxidativephosphorylation | Uncoupled | |||
| Titration | Mal, Glu, Pyr | ADP | Succinate | CCCP | Rotenone | |
| Label | LEAK (NL) | NP | NSP | NSE | SE | |
| Respiration per wetweight [pmol O2/(s*mg)] | Veh PBS | 14.9 (0.7) | 55.7 (7.1) | 88.5 (15.9) | 116.6 (10.5) | 34.6 (4.5) |
| CORT PBS | 16.6 (1.6) | 63.0 (9.3) | 97.7 (11.5) | 124.8 (12.8) | 36.8 (4.5) | |
| Veh PFF | 14.7 (1.8) | 55.7 (7.6) | 88.0 (9.1) | 114.0 (12.2) | 34.8 (4.8) | |
| CORT PFF | 16.8 (1.6) | 63.0 (6.3) | 96.6 (7.2) | 123.2 (10.8) | 36.7 (3.5) | |
| Interaction | F(1,29) = 0.088 | F(1,29) = 0 | F(1,29) = 0 | F(1,29) = 0.016 | F(1,29) = 0.012 | |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||
| CORT factor | F(1,29) = 12.670 | F(1,29) = 7.488 | F(1,29) = 6.983 | F(1,29) = 4.572 | F(1,29) = 1.786 | |
| ** | * | * | * | n.s. | ||
| PFF factor | F(1,29) = 0 | F(1,29) = 0 | F(1,29) = 0.060 | F(1,29) = 0.264 | F(1,29) = 0.004 | |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||
| FCRs (normalizationto NSE) | Veh PBS | 0.13 (0.01) | 0.48 (0.02) | 0.76 (0.03) | 1 | 0.30 (0.01) |
| CORT PBS | 0.13 (0.01) | 0.50 (0.03) | 0.78 (0.02) | 1 | 0.29 (0.02) | |
| Veh PFF | 0.13 (0.01) | 0.48 (0.03) | 0.77 (0.02) | 1 | 0.30 (0.01) | |
| CORT PFF | 0.14 (0.02) | 0.51 (0.03) | 0.78 (0.03) | 1 | 0.30 (0.02) | |
| Interaction | F(1,29) = 0.040 | F(1,29) = 0.021 | F(1,29) = 0.351 | - | F(1,29) = 0.166 | |
| n.s. | n.s. | n.s. | - | n.s. | ||
| CORT factor | F(1,29) = 1.711 | F(1,29) = 6.773 | F(1,29) = 3.784 | - | F(1,29) = 0.250 | |
| n.s. | * | n.s. | - | n.s. | ||
| PFF factor | F(1,29) = 0.163 | F(1,29) = 0.973 | F(1,29) = 0.840 | - | F(1,29) = 0.891 | |
| n.s. | n.s. | n.s. | - | n.s. | ||
| Mitochondrial ROS [pmol/(s*mgwet weight)] | Veh PBS | 0.13 (0.03) | 0.09 (0.03) | - | - | 0.41 (0.17) |
| CORT PBS | 0.13 (0.03) | 0.09 (0.04) | - | - | 0.44 (0.18) | |
| Veh PFF | 0.12 (0.03) | 0.09 (0.02) | - | - | 0.39 (0.13) | |
| Amygdala | ||||||
| State | LEAK | Oxidativephosphorylation | Uncoupled | |||
| Titration | Mal, Glu, Pyr | ADP | Succinate | CCCP | Rotenone | |
| Label | LEAK (NL) | NP | NSP | NSE | SE | |
| CORT PFF | 0.15 (0.03) | 0.09 (0.02) | - | - | 0.40 (0.19) | |
| Interaction | F(1,28) = 1.142 | F(1,27) = 0.095 | - | - | F(1,28) = 0.026 | |
| n.s. | n.s. | - | - | n.s. | ||
| CORT factor | F(1,28) = 1.423 | F(1,27) = 0.203 | - | - | F(1,28) = 0.089 | |
| n.s. | n.s. | - | - | n.s. | ||
| PFF factor | F(1,28) = 0.009 | F(1,27) = 0.159 | - | - | F(1,28) = 0.210 | |
| n.s. | n.s. | - | - | n.s. | ||
Mean values (SD) are given for the different conditions with details on statistical tests (two-way ANOVAs) in the rows below them; *p < 0.05 and **p < 0.01.