Table 2

CT scanning parameters for each specimen used for the different scanning times during the staining optimisation (a.), and subsequent brain and forebrain study (b., c.)

SpecimenVoltage(kV)Amperage(μA)Filter(LE)Source(mm)Detector(mm)Voxel(μm)ObjectivemagnificationBinningExposuretime (s)ProjectionsnumberScanningtime(min)
a. Scan at each staining time point, scout and scan parameters
CP 16053–667631.990.4×2140130
CP 28073–9042.623.430.4×1540160
CP 38073–9042.623.430.4×1540160
CP 48073–9042.623.430.4×1540160
CA 16052–4510021.430.4×2140130
CA 26052–4510021.430.4×2140130
CA 36052–4510021.430.4×2140130
CA 46052–4510021.430.4×2140130
b. Brain scan at optimized staining time, detailed scan parameters
CP 18073–667631.990.4×152501360
CP 28073–9042.623.430.4×152501360
CP 38073–9042.623.430.4×152501360
CP 48073–9042.623.430.4×152501360
CA 26052–4510021.430.4×222501180
CA 36052–4510021.430.4×222501180
CA 46052–4510021.430.4×222501180
c. Forebrain scan at optimized staining time, higher resolution scan parameters
CP 18073–45103110.4×152501380
CP 28073–5410311.880.4×152501393
CP 38073–5410311.880.4×152501393
CP 48073–5410311.880.4×152501393
CA 26052–25145100.4×232501180
CA 36052–25145100.4×232501180
CA 46052–25145100.4×232501180
  • For specimen abbreviations, see Table 1.