Table 2

Ladder-run foot scoring scale

ScoreMetz scale2009Explanation
0Total miss0 points were assigned when the limb completely missed a rung (i.e. it did not touch it), and a fall occurred. Afall was defined as a limb deeply falling in between rungs with body posture and balance being disturbed. 
1Deep slipThe limb was initially placed on a rung, then slipped off as the mouse began to bear weight, causing theanimal to fall. 
2Slight slipThe limb was placed on a rung, then slipped off as the mouse began to bear weight, but it did not resultin a fall or interrupt the step cycle. In this case, the animal was able to maintain balance and continuea coordinated gait. 
3ReplacementThe limb was placed on a rung, but before it was bearing weight it was quickly lifted and placed onanother rung. 
4CorrectionThe limb was aimed for one rung but was then placed on another rung without touching the first one.Alternatively, a score of 4 was recorded if a limb was placed on a rung and was quickly repositionedwhile remaining on the same rung. 
5PartialplacementThe limb was placed on a rung with either wrist or digits of the forelimb or heel or toes of the hindlimb. 
6CorrectplacementThe midplantar surface of a limb was placed on the rung with full weight support.
  • This 6 point scale was used to score the mouse hindlimb positioning when crossing over the ladder-run test, 0 being the complete miss of the hindpaw placement on the ladder rung, up to a maximum score of six with correct paw placement achieved. This scale was used throughout the experiments and was adapted from Metz and Whishaw (2009).