Experimental details of the retrobead injections
| Targetarea | Retrobeadspread into | Bregma(mm) | Dorso-ventral(mm) | Medial-lateral(mm) | Angle(°) | Lateral pialocation(mm) | Volume(nl) | Dilution | Backflowto |
|---|---|---|---|---|---|---|---|---|---|
| Cortex | VO/ LO | 2.2 | –2.3 | 1 | 250 | 1:4 | |||
| Cg | 1.34 | –1.75 | 0.4 | 250 | 1:4 | ||||
| 1.2 | –1 | 0.3 | 250 | Undiluted | |||||
| 1.1 | –1.75 | 0.25 | 250 | 1:4 | |||||
| M1 | 1.35 | –1 | 1.5 | 250 | 1:4 | ||||
| RS | –1.06 | –1 | 0.25 | 150 | 1:4 | ||||
| mV1 | –3.3 | –0.4 | 2.1 | 250 | Undiluted | ||||
| Subcortex | mHab; PV | –1.6 | –2.4 | 0.25 | 150 | Undiluted | |||
| CL, LPMR, MD (MDM,MDC, MDL) | –1.6 | –3.25 | 0.45 | 14 | 1.25 | 150 | Undiluted | Hab, Hip, MPtA | |
| CM, MD (MDC, MDL,MDM), PC, Rh, Sub | –1.7 | –3.75 | 0.3 | 11 | 1 | 150 | Undiluted | Hab, Hip, RS | |
| CM, MD (MDM, MDC),VM | –1.8 | –3.3 | 0.3 | 100 | Undiluted | Hab, Hip, PV | |||
| CL, lHab, IMD, MD(MDM, MDC, MDL), VPPC | –1.94 | –3.3 | 0.13 | 15 | 1 | 150 | 1:4 | Hip, LPtA |
Mice were injected into cortical or subcortical areas using the stated coordinates. Cg: cingulate cortex; CL: centrolateral thalamic nucleus; CM: Central medial thalamic nucleus; Hab: habenula; Hip: hippocampus; IMD: intermediodorsal thalamic nucleus; lHab: lateral habenula; LO: lateral orbital cortex; LPMR: lateral posterior thalamic nucleus; M1: primary motor cortex; MD: mediodorsal thalamus with subnuclei: MDC- Central, MDM: medial, MDL: lateral; mHab: medial habenular nucleus; MPtA: medial parietal association cortex; mV1: monocular primary visual cortex; PC: paracentral thalamic nucleus; PV: paraventricular thalamic nucleus; Rh: rhomboid thalamic nucleus; RS: retrosplenial cortex; Sub: submedius thalamic nucleus; VM: ventromedial thalamic nucleus; VPPC: parvicellular part of the ventral posterior nucleus of the thalamus.