Table 3

Statistical parameters for reproductive measures

nResults
GnRH-GFP-4Di (two-tailed unpairedStudent’s t test)
VO
(4A, left panel)
10 saline
9 CNO
p = 0.8931, t = 0.1365, df = 17
Age at first estrus
(4B, left panel)
10 saline
9 CNO
p = 0.1936, t = 0.1353, df = 17
GnRH-GFP-3Dq (two-way ANOVA)
VO
(4A, right panel)
16 control saline
20 control CNO
12 PNA saline
13 PNA CNO
*Control vs PNA: F(1,57) = 17.11, p = 0.0001
Saline vs CNO: F(1,57) = 0.0897, p = 0.766
Interaction: F(1,57) = 0.0204, p = 0.887
Age at first estrus
(4B, right panel)
15 control saline
19 control CNO
10 PNA saline
10 PNA CNO
*Control vs PNA: F(1,50) = 18.45, p < 0.0001
Saline vs CNO: F(1,50) = 1.349, p = 0.2510
Interaction: F(1,50) = 1.12, p = 0.2950
Estrous cycles
GnRH-GFP-4Di (two-way ANOVA)11 saline
6 CNO
Saline vs CNO: F(1,45) = 0, p > 0.9999
*Cycle stage: F(2,45) = 151.1, p < 0.0001
*Interaction: F(2,45) = 4.654, p = 0.0145
GnRH-GFP-3Dq (three-way ANOVA)6 control saline
11 control CNO
10 PNA saline
14 PNA CNO
*Cycle stage: F(1.084,40.10) = 257.8, p < 0.0001
*Control vs PNA: F(1,37) = 0.0178, p = 0.895
Saline vs CNO: F(1,37) = 1.237, p = 0.2733
*Cycle stage × control vs PNA: F(2,74) = 20.0, p < 0.0001
Cycle stage × saline vs CNO: F(2,74) = 1.085, p = 0.3423
Control vs PNA × saline vs CNO: F(1,37) = 0.0178, p = 0.895
Cycle stage × control vs PNA × saline vs CNO:
F(2,74) = 0.477, p = 0.622