Statistical parameters for reproductive measures
| n | Results | |
|---|---|---|
| GnRH-GFP-4Di (two-tailed unpairedStudent’s t test) | ||
| VO (4A, left panel) | 10 saline 9 CNO | p = 0.8931, t = 0.1365, df = 17 |
| Age at first estrus (4B, left panel) | 10 saline 9 CNO | p = 0.1936, t = 0.1353, df = 17 |
| GnRH-GFP-3Dq (two-way ANOVA) | ||
| VO (4A, right panel) | 16 control saline 20 control CNO 12 PNA saline 13 PNA CNO | *Control vs PNA: F(1,57) = 17.11, p = 0.0001 Saline vs CNO: F(1,57) = 0.0897, p = 0.766 Interaction: F(1,57) = 0.0204, p = 0.887 |
| Age at first estrus (4B, right panel) | 15 control saline 19 control CNO 10 PNA saline 10 PNA CNO | *Control vs PNA: F(1,50) = 18.45, p < 0.0001 Saline vs CNO: F(1,50) = 1.349, p = 0.2510 Interaction: F(1,50) = 1.12, p = 0.2950 |
| Estrous cycles | ||
| GnRH-GFP-4Di (two-way ANOVA) | 11 saline 6 CNO | Saline vs CNO: F(1,45) = 0, p > 0.9999 *Cycle stage: F(2,45) = 151.1, p < 0.0001 *Interaction: F(2,45) = 4.654, p = 0.0145 |
| GnRH-GFP-3Dq (three-way ANOVA) | 6 control saline 11 control CNO 10 PNA saline 14 PNA CNO | *Cycle stage: F(1.084,40.10) = 257.8, p < 0.0001 *Control vs PNA: F(1,37) = 0.0178, p = 0.895 Saline vs CNO: F(1,37) = 1.237, p = 0.2733 *Cycle stage × control vs PNA: F(2,74) = 20.0, p < 0.0001 Cycle stage × saline vs CNO: F(2,74) = 1.085, p = 0.3423 Control vs PNA × saline vs CNO: F(1,37) = 0.0178, p = 0.895 Cycle stage × control vs PNA × saline vs CNO: F(2,74) = 0.477, p = 0.622 |