Table 4

Gene abundance of injured nerve ligand mRNAs in the neonatal nerve scRNA-seq dataset

	Gene abundance (%)
Gene	Epineurial	Endoneurial/perineurial	VSM/pericytes	Endothelial	Schwann cells	Immune cells
Adm^**	5.1	4.7	BT	3.0	BT	BT
Agt^*	2.5	BT	12.2	BT	BT	BT
Angpt1^*	5.1	BT	11.1	BT	BT	BT
Angpt2^*	5.5	BT	18.1	6.9	2.0	BT
Apln	BT	BT	BT	29.0	BT	BT
Bdnf	BT	BT	3.6	BT	BT	BT
Bmp1^**	34.2	21.5	10.9	11.0	10.8	BT
Bmp2	BT	BT	6.7	3.4	BT	8.7
Bmp4^*	3.4	BT	BT	6.9	BT	BT
Bmp5	BT	BT	13.4	BT	BT	BT
Bmp7^**	BT	3.9	BT	BT	BT	BT
Ccl11^**	9.4	49.8	20.0	BT	BT	2.6
Ccl2^*	BT	10.0	9.9	BT	BT	20.0
Ccl24	BT	BT	BT	BT	BT	27.0
Ccl3	BT	BT	BT	BT	BT	16.5
Ccl5	BT	BT	BT	BT	BT	2.6
Ccl7^**	3.4	18.5	2.7	BT	BT	14.8
Ccl9	BT	BT	BT	BT	BT	28.7
Clcf1	BT	BT	BT	2.3	BT	BT
Csf1^**	15.2	4.3	4.8	4.1	2.4	BT
Ctgf^*	7.1	4.0	7.4	7.4	BT	BT
Cxcl1^*	BT	12.4	20.6	2.5	BT	2.6
Cxcl10	BT	BT	BT	BT	2.6	BT
Cxcl12^**	18.9	37.2	23.1	33.8	BT	BT
Cxcl16	BT	BT	BT	4.2	BT	11.3
Cxcl2	BT	BT	BT	BT	BT	4.3
Cxcl9^**	BT	2.1	BT	BT	BT	BT
Dhh	BT	BT	2.3	7.1	25.6	3.5
Dll1	BT	BT	BT	5.3	BT	BT
Dll4	BT	BT	BT	15.2	BT	BT
Ebi3	BT	BT	BT	BT	BT	13.0
Eda^**	7.1	9.0	BT	BT	4.4	BT
Edn3^**	4.1	3.5	BT	BT	BT	BT
Efna1^*	3.1	4.3	BT	34.0	BT	BT
Efna2^**	2.9	3.3	BT	BT	2.9	BT
Efna4^**	2.6	3.6	BT	BT	BT	BT
Efna5^**	6.0	4.0	BT	BT	2.3	BT
Efnb1^**	18.3	30.6	6.3	9.7	9.1	2.6
Efnb2^**	5.4	15.1	4.8	16.3	BT	BT
Fgf1^**	5.1	5.6	3.6	BT	5.4	3.5
Fgf10^**	5.7	BT	BT	BT	BT	BT
Fgf18^**	7.8	BT	BT	BT	BT	BT
Fgf7^**	21.0	10.3	BT	BT	BT	BT
Figf^**	3.8	4.8	BT	BT	BT	BT
Fstl1^**	96.0	92.2	72.9	59.3	43.0	11.3
Gas6^*	22.4	14.3	7.4	24.6	BT	45.2
Gdf10^**	8.4	BT	BT	BT	BT	BT
Gdf11^**	BT	6.6	3.8	2.7	BT	BT
Gmfb^**	9.5	17.6	6.1	13.8	14.1	9.6
Gmfg	BT	BT	BT	6.4	BT	36.5
Grp	BT	BT	BT	2.1	BT	BT
Hbegf^*	BT	2.5	14.3	15.0	19.2	3.5
Igf1^**	56.0	52.0	4.6	16.6	2.0	40.0
Igf2^**	92.3	89.5	54.0	69.7	11.1	25.2
Il15	BT	BT	BT	BT	BT	4.3
Il16	BT	BT	BT	4.6	BT	12.2
Il18	BT	BT	BT	BT	BT	9.6
Il1b	BT	BT	BT	BT	BT	3.5
Il33^**	13.7	16.1	BT	BT	BT	BT
Inha^**	2.6	BT	BT	BT	BT	BT
Inhba^**	4.1	BT	BT	BT	BT	BT
Inhbb	BT	BT	8.0	BT	BT	BT
Jag1^**	18.4	19.1	15.5	11.0	BT	BT
Jag2	BT	BT	BT	7.1	BT	BT
Ltb	BT	BT	BT	BT	BT	2.6
Mdk^**	52.3	68.1	20.2	8.8	20.9	8.7
Metrn^*	3.4	8.7	10.5	5.0	43.2	10.4
Mif^*	17.5	26.4	28.8	26.0	32.8	26.1
Nenf^**	54.3	54.6	39.7	28.8	24.5	BT
Ngf^**	BT	8.7	8.0	BT	BT	0.0
Nov^**	23.8	4.3	3.8	3.4	2.8	3.5
Nppc^**	BT	6.1	BT	BT	BT	BT
Ntf3	BT	BT	2.3	BT	BT	BT
Ntn1^**	22.2	10.0	BT	BT	BT	BT
Osm	BT	BT	BT	BT	BT	3.5
Pdgfa^*	3.4	3.6	30.5	3.0	17.8	BT
Pdgfb	BT	BT	2.9	21.9	BT	BT
Pdgfc	BT	BT	3.2	BT	2.2	2.6
Pf4	BT	BT	BT	BT	BT	61.7
Pgf	BT	BT	3.8	BT	BT	BT
Pthlh^**	7.1	4.0	BT	BT	BT	BT
Ptn^*	29.3	13.9	6.5	15.9	43.4	4.3
Rspo1^**	7.8	6.5	BT	BT	BT	BT
Rtn4^*	31.0	38.2	35.7	40.4	26.8	38.3
Sema3b^*	5.4	6.5	2.3	2.8	34.7	7.8
Sema3c^**	51.7	19.2	BT	BT	7.1	3.5
Sema3d^**	10.4	15.9	BT	BT	BT	BT
Sema3f^*	2.6	BT	BT	2.8	BT	BT
Sema3g	BT	BT	BT	9.6	7.5	BT
Sema4a	BT	BT	BT	BT	BT	10.4
Sema4b	BT	BT	BT	BT	BT	3.5
Sema4c^*	5.1	5.7	2.7	8.1	8.5	5.2
Sema4d	BT	BT	BT	BT	BT	7.8
Sema5a^*	5.4	5.5	12.4	BT	BT	BT
Sema5b^*	4.4	8.6	14.1	BT	2.6	BT
Sema6a^*	4.4	7.9	BT	22.8	4.5	BT
Sema6b	BT	BT	BT	2.5	BT	BT
Sema6c^**	4.9	3.3	2.5	BT	BT	BT
Sema6d^*	2.0	3.0	4.4	6.4	16.7	8.7
Sema7a	BT	BT	2.1	17.3	2.0	BT
Sfrp1^**	50.5	48.5	2.9	4.1	28.0	3.5
Sfrp2^**	17.2	BT	BT	BT	BT	BT
Sfrp4^**	43.7	5.5	BT	BT	BT	2.6
Sfrp5^**	5.4	30.7	BT	BT	8.1	2.6
Tgfb1	BT	BT	BT	10.3	BT	20.9
Tgfb2^*	12.1	2.7	18.3	9.9	BT	BT
Tgfb3^**	21.5	8.3	10.9	BT	BT	BT
Tnf	BT	BT	BT	BT	BT	6.1
Tnfrsf11b^*	2.9	BT	BT	3.9	BT	BT
Tnfsf10	BT	BT	BT	6.4	BT	BT
Tnfsf12^**	8.0	10.8	5.5	6.5	3.2	4.3
Tnfsf9	BT	BT	BT	BT	BT	4.3
Tslp	BT	BT	2.7	2.8	BT	3.5
Vegfa^**	16.0	3.6	3.8	3.2	BT	BT
Vegfb^**	4.0	7.7	6.3	5.5	4.3	6.1
Vegfc	BT	BT	BT	8.5	BT	BT
Wnt11	BT	BT	2.7	BT	BT	BT
Wnt2^**	7.5	BT	BT	BT	BT	BT
Wnt5a^**	17.0	6.8	BT	BT	BT	BT

The neonatal nerve scRNA-seq dataset (Fig. 2E) was analyzed to determine the percentage of cells within a given cell type that detectably expressed (≥2%) the 143 injured nerve ligand mRNAs (Table 2). Cell populations were defined as for the injured nerve analysis. BT = below threshold and indicates that <2% of cells detectably expressed the ligand mRNA. Ligands annotated with one asterisk in the leftmost column were expressed in ≥2% Pdgfra-positive mesenchymal cells and two asterisks indicate ligands with the highest expression in either the epineurial/perineurial or endoneurial Pdgfra-positive mesenchymal cells.
^* >2% expression in Pdgfra-positive cells.
^** >2% expression and highest expression in Pdgfra-positive cells.