Table 3

Gene abundance of injured nerve ligand mRNAs in the uninjured nerve scRNA-seq dataset

Gene abundance (%)
GeneEpineurial/perineurialEndoneurialVSM/pericytesEndothelialSchwann(non-myelinating)Schwann(myelinating)Immune
Adm** 14.48.4BT3.6BTBT2.9
Agt** 2.9BTBTBTBTBT2.9
Angpt1* 12.2BT12.4BTBTBTBT
Angpt2* 2.2BT21.113.6BT6.0BT
Angpt4** 6.2BTBTBTBTBT2.9
AplnBTBTBT7.1BTBTBT
BdnfBTBT3.1BTBTBTBT
Bmp1** 42.016.16.86.24.64.8BT
Bmp2* 3.5BT15.5BTBTBT11.4
Bmp4** 18.66.5BT17.6BTBTBT
Bmp5* 2.46.88.1BTBTBTBT
Bmp7** 3.111.9BTBTBTBTBT
CckBTBT3.1BTBTBTBT
Ccl11** 35.888.322.49.05.33.62.9
Ccl19** 6.2BT5.0BTBTBTBT
Ccl2* 2.216.14.3BTBTBT28.6
Ccl3BTBTBTBTBTBT11.4
Ccl5BTBTBTBTBTBT11.4
Ccl7** 6.035.52.55.02.73.68.6
Ccl9** BT16.6BTBTBT2.414.3
Clcf1BTBTBT4.0BTBT2.9
Csf1** 31.910.04.315.28.82.48.6
Ctgf* 16.62.110.628.8BTBTBT
Cx3cl1BTBTBT11.2BT2.4BT
Cxcl1** 17.552.318.615.011.56.08.6
Cxcl10BTBTBTBTBTBT5.7
Cxcl12* 34.360.039.878.113.43.62.9
Cxcl13** 16.2BTBTBTBTBTBT
Cxcl16** 10.0BTBT3.3BTBT2.9
Cxcl2BTBTBTBTBTBT8.6
Cxcl9BTBTBT3.8BTBTBT
DhhBTBTBT3.813.428.9BT
Dll1BTBTBT10.0BTBTBT
Dll4BTBTBT18.1BTBTBT
Eda** 5.17.7BTBT3.42.42.9
Edn3** 8.4BTBTBTBTBTBT
Efna1* 4.24.03.730.2BTBTBT
Efna2BTBTBTBTBTBT2.9
Efna5** 4.4BTBTBTBTBTBT
Efnb1** 9.514.32.510.56.13.62.9
Efnb2* 3.511.04.315.7BTBTBT
Fgf1* 4.9BT10.6BT10.326.52.9
Fgf10** 9.1BTBTBTBTBTBT
Fgf18** 8.4BTBTBTBTBTBT
Fgf7** 28.86.32.52.4BT8.4BT
Figf** 12.8BTBT3.1BTBTBT
Fstl1** 90.975.547.234.529.810.88.6
Gas6** 61.59.318.049.83.86.02.9
Gdf10** 39.44.0BT3.1BT2.42.9
Gdf11* BT4.24.3BTBTBT2.9
Gmfb* 14.28.65.610.57.615.75.7
GmfgBTBTBT3.1BTBT28.6
Hbegf* BT2.312.417.425.28.45.7
Igf1** 56.943.98.117.62.7BTBT
Igf2* 3.58.67.514.3BTBTBT
Il15* BT4.7BT5.0BTBTBT
Il16BTBTBT3.1BT8.425.7
Il18** 7.13.7BTBTBTBTBT
Il1bBTBTBTBTBTBT8.6
Il33** 29.471.5BT5.03.42.42.9
Il6* 2.42.84.35.2BTBTBT
Inha** 2.0BTBTBTBTBTBT
InhbaBTBT8.1BTBTBTBT
Jag1* 12.44.220.519.3BTBTBT
Jag2BTBTBT9.5BTBTBT
Lif** BT3.0BTBTBTBT2.9
LtbBTBTBTBTBTBT11.4
Mdk** 8.216.4BTBT3.4BT2.9
Metrn* 2.7BT2.5BT14.512.02.9
Mif* 14.814.719.331.013.718.131.4
Nenf** 54.248.628.031.026.719.317.1
Ngf* BT2.611.2BTBTBTBT
Nov** 19.27.96.85.07.315.7BT
Nppc** BT2.6BTBTBTBTBT
Ntf3* 4.0BT11.85.7BTBTBT
Ntn1** 31.212.6BT6.7BTBTBT
OsmBTBTBTBTBTBT8.6
Pdgfa* 7.3BT32.99.814.116.95.7
PdgfbBTBT3.128.1BTBTBT
Pdgfc** 3.3BTBTBTBTBT2.9
Pgf* 2.44.77.5BTBTBTBT
PomcBTBTBT5.0BTBT2.9
Pthlh** 12.67.2BTBTBTBTBT
Ptn* 7.74.0BT45.756.5BT5.7
Rspo1** 8.4BTBTBTBTBTBT
Rtn4** 37.633.421.733.824.021.711.4
Sema3b* 15.532.73.13.144.356.6BT
Sema3c** 38.19.12.53.69.22.4BT
Sema3d** 13.7BTBTBTBTBTBT
Sema3e* 3.5BTBTBT14.5BTBT
Sema3fBTBT2.512.6BTBTBT
Sema3g* BT3.0BT18.82.72.4BT
Sema4aBTBTBTBTBTBT5.7
Sema4b** 2.02.1BTBTBTBTBT
Sema4c* 6.66.3BT11.25.722.9BT
Sema4dBTBTBTBT2.3BT8.6
Sema5a* 2.73.56.8BTBT9.6BT
Sema6a* 2.718.7BT26.22.72.42.9
Sema6b* 2.02.8BT12.4BTBT2.9
Sema6c* BT3.3BTBTBT7.2BT
Sema6d* 3.33.58.19.525.213.3BT
Sema7aBTBTBT28.88.4BT2.9
Sfrp1** 45.415.02.54.06.9BTBT
Sfrp2** 45.15.1BT5.23.86.05.7
Sfrp4** 61.78.63.19.85.36.08.6
Sfrp5* 18.85.83.74.526.031.35.7
TgfaBTBTBT4.0BTBTBT
Tgfb1* 2.03.3BT7.9BTBT17.1
Tgfb2** 8.22.85.66.0BTBTBT
Tgfb3** 16.82.316.13.1BTBTBT
TnfBTBTBTBTBTBT11.4
Tnfsf10BTBTBT18.6BTBTBT
Tnfsf12** 13.317.86.212.69.99.65.7
Tnfsf14BTBTBTBTBTBT8.6
Tnfsf9* BT4.0BTBT5.7BTBT
Tslp** 3.5BTBTBTBTBT2.9
Vegfa** 14.27.98.16.2BT2.42.9
Vegfb* 5.54.76.25.73.47.2BT
Vegfc* 3.3BTBT6.2BTBTBT
Wnt11** 6.6BTBTBTBTBT2.9
Wnt2** 6.9BTBTBTBTBTBT
Wnt5a** 17.510.3BTBTBTBTBT
  • The uninjured nerve scRNA-seq dataset (Fig. 2C) was analyzed to determine the percentage of cells within a given cell type that detectably expressed (≥2%) the 143 injured nerve ligand mRNAs (Table 2). Cell populations were defined as for the injured nerve analysis except that Schwann cells were divided into myelinating (cluster 8) and non-myelinating (cluster 4) cells. BT = below threshold and indicates that <2% of cells detectably expressed the ligand mRNA. Ligands annotated with one asterisk in the leftmost column were expressed in ≥2% Pdgfra-positive mesenchymal cells and two asterisks indicate ligands with the highest expression in either the epineurial/perineurial or endoneurial Pdgfra-positive mesenchymal cells.

  • * >2% expression in Pdgfra-positive cells.

  • ** >2% expression and highest expression in Pdgfra-positive cells.