Gene abundance of injured nerve ligand mRNAs in the combined injured, uninjured and neonatal Schwann cell scRNA-seq dataset
| Gene abundance (%) | Fold change | ||||
|---|---|---|---|---|---|
| Gene | Neonatal | Uninjured (myelinating) | Uninjured (non-myelinating) | Injured | Injured:uninjured (non-myel.) |
| Angpt2 | 2.0 | 6.4 | BT (0.4) | BT (1.7) | 4.1 |
| Artn | BT (0.0) | BT (0.0) | BT (0.0) | 3.1* | >3.1 |
| Bdnf | BT (0.0) | BT (0.0) | BT (0.0) | 9.3* | >9.3 |
| Bmp1 | 10.9 | 3.8 | 4.9 | 46.1* | 9.3 |
| Btc | BT (0.8) | BT (0.0) | BT (0.0) | 58.8* | >58.8 |
| Ccl11 | BT (0.8) | 3.8 | 4.5 | 5.6* | 1.2 |
| Ccl2 | BT (0.7) | BT (0.0) | BT (1.6) | 12.7* | 7.7 |
| Ccl3 | BT (0.0) | BT (0.0) | BT (0.0) | 3.9* | >3.9 |
| Ccl7 | BT (0.2) | 2.6 | 2.9 | 6.1* | 2.1 |
| Ccl9 | BT (0.0) | 2.6 | BT (0.4) | 6.6* | 16.1 |
| Clcf1 | BT (0.2) | BT (0.0) | BT (0.0) | 18.6* | >18.6 |
| Crlf1 | BT (1.3) | BT (0.0) | 2.5 | 30.5* | 12.4 |
| Csf1 | 2.4 | BT (1.3) | 9.1 | 9.7* | 1.1 |
| Ctgf | BT (0.1) | BT (0.0) | BT (0.0) | 3.4* | >3.4 |
| Cx3cl1 | BT (0.0) | 2.6 | BT (0.0) | BT (0.2) | - |
| Cxcl1 | BT (1.4) | 6.4 | 11.9 | 10.7 | 0.9 |
| Cxcl10 | 2.6 | BT (0.0) | BT (0.4) | 5.9* | 14.4 |
| Cxcl12 | BT (1.4) | 2.6 | 12.3 | 5.4 | 0.4 |
| Cxcl2 | BT (0.1) | BT (0.0) | BT (0.0) | 7.1* | >7.1 |
| Dhh | 25.3 | 28.2 | 11.9 | 26.8 | 2.2 |
| Eda | 4.4 | 2.6 | 3.7 | 2.9 | 0.8 |
| Efna2 | 2.8 | BT (1.3) | BT (0.8) | 3.2* | 3.9 |
| Efna4 | BT (1.0) | BT (1.3) | BT (0.0) | 4.9* | >4.9 |
| Efna5 | 2.2 | BT (0.0) | BT (0.0) | BT (0.3) | - |
| Efnb1 | 8.9 | 2.6 | 7.0 | 10.0* | 1.4 |
| Efnb2 | BT (0.6) | BT (0.0) | BT (0.4) | 3.9* | 9.5 |
| Fgf1 | 5.4 | 28.2 | 10.7 | BT (1.5) | 0.1 |
| Fgf5 | BT (0.0) | BT (0.0) | BT (0.0) | 18.3* | >18.3 |
| Fgf7 | BT (1.3) | 9.0 | BT (0.8) | 12.9* | 15.7 |
| Fstl1 | 42.6 | 9.0 | 30.9 | 29.0 | 0.9 |
| Gas6 | BT (0.6) | 6.4 | 2.9 | 2.5 | 0.9 |
| Gdf11 | BT (1.2) | BT (0.0) | BT (0.4) | 3.1* | 7.4 |
| Gdnf | BT (0.0) | BT (0.0) | BT (0.0) | 20.5* | >20.5 |
| Gmfb | 14.2 | 16.7 | 7.0 | 21.9* | 3.1 |
| Hbegf | 19.0 | 9.0 | 26.7 | 39.0* | 1.5 |
| Igf1 | 2.1 | BT (1.3) | 3.3 | 15.4* | 4.7 |
| Igf2 | 11.2 | BT (1.3) | BT (1.2) | 3.6 | 2.9 |
| Il16 | BT (0.7) | 7.7 | BT (0.4) | BT (0.3) | 0.8 |
| Il18 | BT (0.3) | BT (0.0) | 2.1 | BT (0.5) | 0.2 |
| Il1b | BT (0.0) | BT (0.0) | BT (0.0) | 3.7* | >3.7 |
| Il33 | BT (0.2) | 2.6 | 2.9 | 4.2* | 1.5 |
| Jag1 | BT (0.9) | BT (1.3) | BT (1.2) | 6.9* | 5.6 |
| Lif | BT (0.0) | BT (0.0) | BT (0.0) | 4.9* | >4.9 |
| Mdk | 20.6 | BT (1.3) | 3.3 | 25.3* | 7.7 |
| Metrn | 42.8 | 11.5 | 14.0 | 53.6* | 3.8 |
| Mif | 32.5 | 17.9 | 11.9 | 26.1 | 2.2 |
| Nenf | 24.3 | 19.2 | 27.2 | 47.3* | 1.7 |
| Nov | 2.7 | 14.1 | 7.8 | 4.2 | 0.5 |
| Ntn1 | BT (0.6) | BT (0.0) | BT (0.8) | 3.2* | 3.9 |
| Pdgfa | 17.5 | 15.4 | 15.6 | 47.5* | 3.0 |
| Pdgfb | BT (0.2) | BT (0.0) | BT (0.0) | 2.2* | >2.2 |
| Pdgfc | 2.2 | BT (1.3) | BT (1.2) | BT (1.0) | 0.8 |
| Ptn | 42.9 | BT (0.0) | 57.2 | 47.3 | 0.8 |
| Rtn4 | 26.7 | 21.8 | 24.3 | 70.7* | 2.9 |
| Sema3b | 34.4 | 56.4 | 43.2 | 51.4 | 1.2 |
| Sema3c | 7.1 | BT (1.3) | 9.5 | 19.5* | 2.1 |
| Sema3e | BT (1.0) | BT (0.0) | 14.4 | 29.2* | 2.0 |
| Sema3f | BT (0.4) | BT (0.0) | 2.1 | 2.9* | 1.4 |
| Sema3g | 7.5 | 2.6 | BT (1.6) | 8.1* | 4.9 |
| Sema4b | BT (0.4) | BT (0.0) | BT (0.8) | 7.1* | 8.6 |
| Sema4c | 8.5 | 21.8 | 5.3 | 15.4 | 2.9 |
| Sema4d | BT (0.9) | BT (0.0) | 2.1 | BT (1.0) | 0.5 |
| Sema4f | BT (0.0) | BT (0.0) | BT (0.0) | 17.1* | >17.1 |
| Sema5a | BT (0.9) | 10.3 | BT (0.0) | BT (1.9) | >1.9 |
| Sema5b | 2.6 | BT (0.0) | BT (0.0) | BT (0.2) | - |
| Sema6a | 4.5 | 2.6 | 2.9 | 8.0* | 2.8 |
| Sema6c | BT (1.3) | 7.7 | BT (1.2) | 2.0 | 1.6 |
| Sema6d | 16.5 | 11.5 | 25.5 | 14.1 | 0.6 |
| Sema7a | 2.1 | BT (0.0) | 9.5 | 17.1* | 1.8 |
| Sfrp1 | 27.6 | BT (0.0) | 7.0 | 8.0 | 1.1 |
| Sfrp2 | BT (0.4) | 5.1 | 3.7 | 2.0 | 0.5 |
| Sfrp4 | BT (1.3) | 6.4 | 4.9 | 8.8* | 1.8 |
| Sfrp5 | 8.1 | 30.8 | 28.0 | 5.1 | 0.2 |
| Shh | BT (0.0) | BT (0.0) | BT (0.0) | 12.0* | >12.0 |
| Tgfb1 | BT (0.9) | BT (1.3) | BT (0.8) | 9.8* | 11.9 |
| Tgfb2 | BT (0.6) | BT (0.0) | BT (0.8) | 6.1* | 7.4 |
| Tgfb3 | BT (1.8) | BT (1.3) | BT (1.6) | 4.2* | 2.6 |
| Tnfsf12 | 3.2 | 9.0 | 9.5 | 6.3 | 0.7 |
| Tnfsf9 | BT (0.6) | BT (1.3) | 5.3 | BT (0.7) | 0.1 |
| Ucn2 | BT (0.1) | BT (0.0) | BT (0.0) | 14.1* | >14.1 |
| Vegfa | BT (1.1) | 2.6 | BT (0.8) | 2.4 | 2.9 |
| Vegfb | 4.2 | 7.7 | 3.3 | 7.5 | 2.3 |
The combined Schwann cell scRNA-seq dataset (Fig. 3A,B) was analyzed to determine the percentage of cells within the different Schwann cell clusters that detectably expressed (≥2%) the 143 injured nerve ligand mRNAs (Table 2). Also shown is the difference, expressed as fold change, in the percentage of positive cells in the injured versus uninjured, non-myelinating Schwann cell clusters. BT = below threshold and indicates that <2% of cells detectably expressed the ligand mRNA. Also shown are the absolute values, since these were used to determine the fold changes. Neonatal includes cells in clusters 0, 1, 2, and 5, uninjured myelinating includes cluster 6 cells, uninjured non-myelinating includes cluster 4 cells, and injured includes cluster 3 cells. Asterisks indicate ligand mRNAs with the highest expression in the injured Schwann cell cluster.
*Injured, highest expression.