Figure | Data structure | Type of test | Statistical value | p value |
---|---|---|---|---|

2A von Frey pooled | Two factors (group and time point) | Two-way ANOVA | F_{(2,44)} = 41.0 | <0.001 |

2B withdrawal score pooled | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,44)} = 123.3 | <0.001 |

2C von Frey treatment | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(3,34)} = 37.95 | <0.001 |

2D withdrawal score | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(3,34)} = 52.9 | <0.001 |

3A Shannon baseline | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 2.745 | 0.096 |

3A Shannon post-treatment | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 87.15 | <0.001 |

3A Chao1 baseline | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 1.52 | 0.251 |

3A Chao1 posttreatment | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 6.873 | <0.008 |

Bacteroidetes post-treatment | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 8,844 | <0.003 |

Firmicutes post-treatment | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,15)} = 27.43 | <0.001 |

3F caecum weight | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(4,42)} = 52.74 | <0.001 |

4 (BLA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 22.3 | <0.001 |

4 (PAG) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 16.9 | <0.001 |

4 (LC) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 25.2 | <0.001 |

4 (CeA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 73.97 | <0.001 |

4 (PVT) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 23 | <0.001 |

4 (AI) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 5.92 | <0.001 |

4 (BNST) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 55.7 | <0.001 |

4 (LHb) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 37.3 | <0.001 |

5 (BLA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 18.8 | <0.001 |

5 (PAG) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 32.6 | <0.001 |

5 (LC) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 6.79 | <0.005 |

5 (CeA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 5.13 | <0.014 |

5 (PVT) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 1.18 | <0.325 |

5 (AI) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 1.18 | <0.324 |

5 (BNST) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 2.24 | <0.129 |

5 (LHb) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,24)} = 2.71 | <0.87 |

6 (BLA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 2.45 | <0.106 |

6 (PAG) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 17.6 | <0.001 |

6 (LC) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 17.7 | <0.001 |

6 (CeA) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 17.9 | <0.001 |

6 (PVT) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 24.1 | <0.001 |

6 (AI) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 3.87 | <0.034 |

6 (BNST) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 21.6 | <0.001 |

6 (LHb) | One factor (treatment) | One-way ANOVA Student–Newman–Keuls post hoc test | F_{(2,26)} = 38.1 | <0.001 |