Primers used for creation of Drosophila VAChT UTR with modified PAM sites (5’: a, b, c, d and 3’: e, f, g, h) and modified PolyQ regions (5’: i, j, k, l and 3’: m, n, o, p)
| Sequence | Use | ||
|---|---|---|---|
| ATCGGGCGCGCCGAATTCATGCTTGGGTCGACTTAAGCTC | a | a + b | (5’PAM) |
| ACAAAGTTCTGATGCAGTTTCTTTGG | b | ||
| CCAAAGAAACTGCATCAGAACTTTGT | c | c + d | |
| CTTAAATAGTCGGGTATAATCGGTACTA | d | ||
| GTACACTAGTTCGTGTTCTTTTGCACACCTCC | e | e + f | (3’PAM) |
| ACGTACCACTTGGCTATATGTCTATA | f | ||
| TATAGACATATAGCCAAGTGGTACGT | g | g + h | |
| GCTACTCGAGAAGTCCGCCACAATGACAACC | h | ||
| GTGCCTACTGGACGGGCT | i | i + j | VAChTΔQ |
| CAGGACCTCTGCTCTGGACGAAGGGATTGGCCACACGG | j | ||
| CCGTGTGGCCAATCCCTTCGTCCAGAGCAGAGGTCCTG | k | k + l | |
| GCTATTAATTAACATATGTAGGAGTATCTGTTCGGGGCAA | l | ||
| GTGCCTACTGGACGGGCT | m | m + n | VAChT+Q |
| CTGCTGCTGCTGCTGTTGTTGTTGCTGCTGCTGCTGCTGCTG | n | ||
| CAGCAGCAGCAGCAACAACAACAACAACAGCAGCAGGTCCAGAGC | o | o + p | |
| GCTATTAATTAACATATGTAGGAGTATCTGTTCGGGGCAA | p | ||