Primer sequences for qPCR or to create probes for in situ hybridization
| Test | Gene | Reference | Primer | Primer sequences (5′–3’) |
|---|---|---|---|---|
| mRNA in situ hybridization | dmrt1a | AY157562.1 | F R |
GGCCACAAACGCTTCTGTAA
TTGTAACTGGCAGCTGGAGA |
| dmrt2a | NM_130952.1 | F R |
ATCCACCCAGTCCAACTCAG
TTCCTCCAGCAGCTCCTTAC | |
| dmrt3a | NM_001005779.2 | F R |
CTCTGGCACCTTTGGAAACC
TTGTGGGCAGGGAAGATCTT | |
| wt1a | NM_131046.1 | F R |
TGACCCAACTTGACTTTGCG
CTGGAGGAGGAACGGGATAC | |
| qPCR | dmrt1a | AY157562.1 | F R |
GCCAGTGTCAGAAATGCAGA
TGAACCGGAAAGGTTAATCG |
| dmrt2a | NM_130952.1 | F R |
CCCGCGATTTGTAATGTGGC
GAGGGTGACTTTCGGTGGAG | |
| dmrt3a | NM_001005779.2 | F R |
TGGCAGTGACAGAGAACCAG
GGGTCAGAGCAGGATTTTGA | |
| wt1a | NM_131046.1 | F R |
GGAAGTCAAGCTCTGCTGCT
AACCTCCTGGATGGCTCTTT | |
| elfa | AY422992 | F R |
CTTCTCAGGCTGACTGTGC
CCGCTAGCATTACCCTCC | |
| rpl13 | NM_212784 | F R |
TCTGGAGGACTGTAAGAGGTATGC
AGACGCACAATCTTGAGAGCAG |
F, Forward; R, reverse.