Immunostaining patterns and RNA levels of TFs in vestibulospinal neurons
| Gene | Species | Groups immunostained | LVST TPM | cMVST TPM |
|---|---|---|---|---|
| Phox2b | Mouse | LVST | 678 ± 126 | 9 ± 3 |
| Chicken | LVST | 16 ± 20 | 0 ± 0 | |
| Lbx1 | Mouse | LVST + cMVST | 36 ± 30 | 0 |
| Chicken | LVST | 0 ± 0 | 0 ± 0 | |
| Maf | Mouse | LVST + cMVST | 495 ± 165 | 390 ± 125 |
| Chicken | LVST + cMVST | 55 ± 35 | 82 ± 23 | |
| Esrrg | Mouse | LVST + cMVST | 753 ± 124 | 588 ± 272 |
| Chicken | LVST + cMVST | 143 ± 106 | 177 ± 85 | |
| Pou3f1 | Mouse | LVST + cMVST | 103 ± 41 | 5 ± 2 |
| Chicken | N/A | 0 ± 0 | 0 ± 0 | |
| Onecut1 | Mouse | LVST + cMVST | 40 ± 23 | 175 ± 75 |
| Chicken | LVST + cMVST | 50 ± 28 | 92 ± 49 | |
| Onecut2 | Mouse | LVST + cMVST | 112 ± 20 | 275 ± 128 |
| Chicken | N/A | 2 ± 3 | 8 ± 6 | |
| Onecut3 | Mouse | LVST + cMVST | 87 ± 61 | 131 ± 99 |
| Chicken | N/A | 10 ± 10 | 6 ± 5 | |
| Lhx1 | Mouse | cMVST | 0 | 115 ± 35 |
| Chicken | cMVST | 0 ± 0 | 200 ± 121 | |
| Lhx5 | Mouse | cMVST | 0 | 57 ± 19 |
| Chicken | cMVST | 1 ± 2 | 18 ± 14 | |
| Foxp2 | Mouse | cMVST | 0 | 59 ± 27 |
| Chicken | cMVST | 0 ± 0 | 65 ± 15 | |
| Evx2 | Mouse | cMVST | 0 | 12 ± 10 |
| Chicken | N/A | 0 ± 0 | 9 ± 8 |
Mean TPM values (±SD) of TFs validated by immunohistofluorescence, showing a strong correlation of VS group RNA levels and immunostaining. A value of 0 indicates no detection by RNAseq. N/A, Not assessed by immunohistofluorescence because of lack of appropriate antibodies.