Reagent | Source | Identifier |
---|---|---|
Antibodies | ||
Mouse anti-tubulin beta III | EMD Millipore | MAB5564 |
Goat anti-mouse Alexa Fluor 488 | Jackson ImmunoResearch | 115-545-003 |
Goat anti-mouse HRP | Jackson ImmunoResearch | 115-053-146 |
Mice | ||
CD1 | Charles River | IMSR catalog #CRL:22, RRID:IMSR_CRL:22 |
B6.129X1-Trpv1tm1Jul/J | The Jackson Laboratories | 3834761, RRID:MGI:3834761 |
Software | ||
Prism 6 | GraphPad | RRID:SCR_002798 |
Inkscape 0.91 | The Inkscape Project | RRID:SCR_014479 |
FIJI | NIH | RRID:SCR_002285 |
Axoquant2.0 | Johnstone et al. (2018) | http://www.github.com/BarkerLabUBC |
RStudio 1.1.442 | RStudio | RRID:SCR_000432 |
ZEN 2 | Zeiss | RRID:SCR_013672 |
LAS X | Leica | RRID:SCR_013673 |
Culture reagents | ||
Neurobasal | Gibco | 21103049 |
B-27 | Gibco | 17504-044 |
Penicillin/streptomycin | Gibco | 15140122 |
GlutaMax | Gibco | 35050-061 |
NGF | CedarLane | CLMCNET-001 |
Poly-D-lysine | Sigma | P6407-5MG |
Laminin/entactin | Corning | 08-774-555 |
Collagen | PurCol | 5005-100ML |
Oligonucleotides | ||
For TrpV1 genotyping (wild-type forward) TGGCTCATATTTGCCTTCAG | Invitrogen | 19922 |
For TrpV1 genotyping (mutant forward) TAAAGCGCATGCTCCAGACT | Invitrogen | oIMR1627 |
For TrpV1 genotyping (in-common reverse) CAGCCCTAGGAGTTGATGGA | Invitrogen | 19923 |