Table 1.

Materials list

ReagentSourceIdentifier
Antibodies
Mouse anti-tubulin beta IIIEMD MilliporeMAB5564
Goat anti-mouse Alexa Fluor 488Jackson ImmunoResearch115-545-003
Goat anti-mouse HRPJackson ImmunoResearch115-053-146
Mice
CD1Charles RiverIMSR catalog #CRL:22, RRID:IMSR_CRL:22
B6.129X1-Trpv1tm1Jul/JThe Jackson Laboratories3834761, RRID:MGI:3834761
Software
Prism 6GraphPadRRID:SCR_002798
Inkscape 0.91The Inkscape ProjectRRID:SCR_014479
FIJINIHRRID:SCR_002285
Axoquant2.0 Johnstone et al. (2018) http://www.github.com/BarkerLabUBC
RStudio 1.1.442RStudioRRID:SCR_000432
ZEN 2ZeissRRID:SCR_013672
LAS XLeicaRRID:SCR_013673
Culture reagents
NeurobasalGibco21103049
B-27Gibco17504-044
Penicillin/streptomycinGibco15140122
GlutaMaxGibco35050-061
NGFCedarLaneCLMCNET-001
Poly-D-lysineSigmaP6407-5MG
Laminin/entactinCorning08-774-555
CollagenPurCol5005-100ML
Oligonucleotides
For TrpV1 genotyping (wild-type forward)
TGGCTCATATTTGCCTTCAG
Invitrogen19922
For TrpV1 genotyping (mutant forward) TAAAGCGCATGCTCCAGACTInvitrogenoIMR1627
For TrpV1 genotyping (in-common reverse) CAGCCCTAGGAGTTGATGGAInvitrogen19923