Table 2

Commercially available primary antibodies used to identify cerebellar cell types: immunogens, sources, RRIDs, and working dilutions

Cerebellar cell type markers
AntibodyImmunogenSourceRRIDWorking dilution
Mouse anti-SMI312 (pan-axonal neurofilament cocktail)SMI 312 is directed against highly phosphorylated axonal epitopes of neurofilamentsBioLegend, #837904, monoclonal AB_2566782 1:1000
Rabbit anti-Pax-6Peptide QVPGSEPDMSQYWPRLQ from the C terminus of mouse Pax-6 proteinBioLegend, clone poly-19013, #901301, polyclonal AB_2565003 1:600 After antigen retrieval
Goat anti-RORαPeptide mapping at the C terminus of RORα1 of human originSanta Cruz, sc-6062, polyclonal AB_655755 1:100 at E17.5–P0 1:300 at P3–P20
Mouse anti- TuJ-1 (neuron-specific beta-III tubulin)Monoclonal mouse IgG2A clone #TuJ-1; detects mammalian and chicken neuron-specific beta–III tubulin; raised against rat brain-derived microtubulesR&D Systems, #MAB1195, monoclonal AB_357520 1:1000 at 1 DIV
Mouse anti-CalbMonoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gutSwant, #300, monoclonal AB_10000347 1:500 at P0–P5 1:1000 at P6–P15 1:3000 at P16–adult
Rabbit anti-CalbAgainst recombinant rat calbindin D-28kSwant, #CB38, polyclonal AB_2721225