Primers used for cloning
| Name | Sequence | Template |
|---|---|---|
| Cx36-EGFP and GCaMP cloning | ||
| T7 extended | GTAATACGACTCACTATAGGGCGAA | MsCx36 cDNA |
| JOB 284 | CCAAACTTGGGTACCCACACATAGGC | MsCx36 cDNA |
| JOB 285 | CCAAACCTTTGATCACACACATAGGC | MsCx36 cDNA |
| PB513B-1 modification | ||
| KBM 1 | AATTAATGACCTGCAGGTCGACGACTGCATAGGGTTAC | pIRES DsRedT3-KR24 |
| KBM 2 | GCCGGGATTCTCCTCCACGATCCATTATCATCGTGTTTTTCAA | pIRES DsRedT3-KR24 |
| KBM 3 | CGCGCCCGCCGCCCTA | PB513B-1 |
| KBM 4 | TGCAGGTCATTAATTAAGGTGGCGTCTAGCGTAGGCG | PB513B-1 |
| Cx36-GCaMP – NR1 dual vector cloning | ||
| KBM 7 | AGCGAATTCGAATTTCAGCGATGGGGGAATGG | Cx36-GCaMP |
| KBM 8 | CCGCGGATCCGATTTTACGCCTTAAGATACATTGATGAGTT | Cx36-GCaMP |
| KBM 405 | AGACGCCACCTTAATTAACGGAGCTCATGAGCACCATG | NR1 |
| KBM 406 | ATGCAGTCGTCGACCTGCAGGTCCTCAGCTCTCCCTATGACGG | NR1 |
| NR2 cloning | ||
| KBM 11 | GCTAGCGAATTCGAATTTCTCTCCACAGGTGTCCACTCC | NR2C |
| KBM 12 | CGGCCGCGGATCCGATTTGGTGCTGCGCCGAATTA | NR2C |
| KBM 13 | GCTAGCGAATTCGAATTTGCTAGCGGCAGATTGGG | NR2A |
| KBM 14 | CGGCCGCGGATCCGATTTCCAGCTGGTTCTTTCCGC | NR2A |
| KBM 15 | GCTAGCGAATTCGAATTTGTGGTGGGCTGAAGACTCCTTA | NR2B |
| KBM 16 | CGGCCGCGGATCCGATTTCCATAGAGCCCACCGCATC | NR2B |