Statistical analysis summary
| Figure | Test comparison | Statistical test | Result | Post hoc test | post-hoc comparison | Adjusted P value | |
|---|---|---|---|---|---|---|---|
| 2A | Mouth-hook contraction | Comparisons between tastants | One-way ANOVA | F(3,102) = 7.901, P < 0.0001 | Dunnett's multiple comparison test | Control vs CAF | 0.001 |
| Control vs DEN | >0.999 | ||||||
| Control vs NIC | <0.001 | ||||||
| Bending | F(3,102) = 8.846, P < 0.0001 | Control vs CAF | 0.092 | ||||
| Control vs DEN | 0.026 | ||||||
| Control vs NIC | 0.026 | ||||||
| Body wall contraction | F(3,102) = 16.94, P < 0.0001 | Control vs CAF | 0.598 | ||||
| Control vs DEN | 0.628 | ||||||
| Control vs NIC | <0.001 | ||||||
| 2B | Mouth-hook contraction | Comparisons between tastants | One-way ANOVA | F(3,76) = 4.355, P = 0.0069 | Dunnett's multiple comparison test | Control vs CAF | 0.473 |
| Control vs DEN | 0.060 | ||||||
| Control vs NIC | 0.995 | ||||||
| Bending | F(3,76) = 4.773, P = 0.0042 | Control vs CAF | 0.312 | ||||
| Control vs DEN | 0.923 | ||||||
| Control vs NIC | 0.078 | ||||||
| Body wall contraction | F(3,76) = 11.90, P < 0.0001 | Control vs CAF | >0.999 | ||||
| Control vs DEN | 0.765 | ||||||
| Control vs NIC | <0.001 | ||||||
| 2C | Mouth-hook contraction | Comparisons between tastants | One-way ANOVA | F(2,56) = 0.2104, P = 0.8109 | Dunnett's multiple comparison test | Control vs SUC | >0.999 |
| Control vs FRU | 0.793 | ||||||
| Bending | Kruskal–Wallis test | 0.9784, P = 0.6131 | Dunn's multiple comparison test | Control vs SUC | >0.05 | ||
| Control vs FRU | >0.05 | ||||||
| Body wall contraction | One-way ANOVA | F(2,56) = 0.1099, P = 0.8961 | Dunnett's multiple comparison test | Control vs SUC | 0.990 | ||
| Control vs FRU | 0.863 | ||||||
| 3A | Comparisons between genotypes | Kruskal–Wallis test | 39.44, P < 0.0001 | Dunn's multiple comparison test | UAS-Kir2.1/+ vs Gr33a>Kir2.1 | <0.05 | |
| Gr33a-GAL4/+ vs Gr33a>Kir2.1 | <0.05 | ||||||
| UAS-Kir2.1/+ vs Gr39b>Kir2.1 | <0.05 | ||||||
| Gr39b-GAL4/+ vs Gr39b>Kir2.1 | <0.01 | ||||||
| UAS-Kir2.1/+ vs Gr23a>Kir2.1 | >0.05 | ||||||
| Gr23a-GAL4/+ vs Gr23a>Kir2.1 | >0.05 | ||||||
| UAS-Kir2.1/+ vs Gr22a>Kir2.1 | >0.05 | ||||||
| Gr22a-GAL4/+ vs Gr22a>Kir2.1 | >0.05 | ||||||
| UAS-Kir2.1/+ vs Gr36b>Kir2.1 | >0.05 | ||||||
| Gr36b-GAL4/+ vs Gr36b>Kir2.1 | >0.05 | ||||||
| 3B | Interaction between genotype and chemical | Two-way ANOVA | F(9,356) = 4.149, P < 0.0001 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (Gr33a-GAL4/+) | <0.05 | |
| Control vs 100 mM CAF (Gr33a>Kir2.1) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr39b-GAL4/+) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr39b>kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr23a-GAL4/+) | <0.01 | ||||||
| Control vs 100 mM CAF (Gr23a>kir2.1) | <0.01 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b-GAL4/+) | <0.01 | ||||||
| Control vs 100 mM CAF (Gr36b>kir2.1) | >0.05 | ||||||
| 3C | Interaction between genotype and chemical | Two-way ANOVA | F(9,356) = 1.837, P = 0.0606 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (Gr33a-GAL4/+) | >0.05 | |
| Control vs 100 mM CAF (Gr33a>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr39b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr39b>kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr23a-GAL4/+) | <0.01 | ||||||
| Control vs 100 mM CAF (Gr23a>kir2.1) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b>kir2.1) | >0.05 | ||||||
| 4A | Comparisons between genotypes | One-way ANOVA | F(10,64) = 15.38, P < 0.0001 | Newman–Keuls multiple comparison test | UAS-Gr33a/+ vs Gr33a>Gr33a | <0.001 | |
| Gr33a-GAL4/+ vs Gr33a>Gr33a | <0.001 | ||||||
| UAS-Gr33a/+ vs Gr39b>Gr33a | <0.001 | ||||||
| Gr39b-GAL4/+ vs Gr39b>Gr33a | <0.001 | ||||||
| UAS-Gr33a/+ vs Gr22d>Gr33a | <0.001 | ||||||
| Gr22d-GAL4/+ vs Gr22d>Gr33a | <0.001 | ||||||
| UAS-Gr33a/+ vs Gr22a>Gr33a | >0.05 | ||||||
| Gr22a-GAL4/+ vs Gr22a>Gr33a | >0.05 | ||||||
| UAS-Gr33a/+ vs Gr36b>Gr33a | >0.05 | ||||||
| Gr36b-GAL4/+ vs Gr36b>Gr33a | >0.05 | ||||||
| 4B | Interaction between genotype and chemical | Two-way ANOVA | F(9,402) = 2.597, P = 0.0064 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (Gr33a-GAL4/+) | >0.05 | |
| Control vs 100 mM CAF (Gr33a>Gr33a) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr39b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr39b>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22d-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22d>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b>Gr33a) | >0.05 | ||||||
| 4C | Interaction between genotype and chemical | Two-way ANOVA | F(9,402) = 2.084, P = 0.0299 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (Gr33a-GAL4/+) | >0.05 | |
| Control vs 100 mM CAF (Gr33a>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr39b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr39b>Gr33a) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr22d-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22d>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>Gr33a) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr36b>Gr33a) | >0.05 | ||||||
| 5A | Comparisons between genotypes | One-way ANOVA | F(13,106) = 7.609, P < 0.0001 | Uncorrected Fisher's LSD test | C7-GAL4/+ vs C7>Kir2.1 | 0.032 | |
| Gr22a-GAL4/+ vs Gr22a>Kir2.1 | 0.002 | ||||||
| C7,Gr22a-GAL4/+ vs C7,Gr22a>kir2.1 | 0.001 | ||||||
| Gr28b.e-GAL4/+ vs Gr28b.e>Kir2.1 | 0.005 | ||||||
| C7,Gr28b.e-GAL4/+ vs C7,Gr28b.e>kir2.1 | <0.001 | ||||||
| Gr59c-GAL4/+ vs Gr59c>Kir2.1 | 0.368 | ||||||
| C7,Gr59c-GAL4/+ vs C7,Gr59c>kir2.1 | <0.001 | ||||||
| C7>Kir2.1 vs C7,Gr22a>Kir2.1 | 0.019 | ||||||
| Gr22a>Kir2.1 vs C7,Gr22a>Kir2.1 | 0.002 | ||||||
| C7>Kir2.1 vs C7,Gr28b.e>Kir2.1 | 0.044 | ||||||
| Gr28b.e>Kir2.1 vs C7,Gr28b.e>Kir2.1 | 0.012 | ||||||
| C7>Kir2.1 vs C7,Gr59c>Kir2.1 | 0.245 | ||||||
| Gr59c>Kir2.1 vs C7,Gr59c>Kir2.1 | 0.018 | ||||||
| 5B | Interaction between genotype and chemical | Two-way ANOVA | F(13,521) = 0.7760, P = 0.6860 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (C7-GAL4/+) | >0.05 | |
| Control vs 100 mM CAF (C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a,C7-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a,C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e,C7-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e,C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c,C7-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c,C7>Kir2.1) | >0.05 | ||||||
| 5C | Interaction between genotype and chemical | Two-way ANOVA | F(13,521) = 1.619, P = 0.0761 | Bonferroni's multiple comparison test | Control vs 100 mM CAF (C7-GAL4/+) | >0.05 | |
| Control vs 100 mM CAF (C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a,C7-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr22a,C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e-GAL4/+) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e,C7-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr28b.e,C7>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c-GAL4/+) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c>Kir2.1) | >0.05 | ||||||
| Control vs 100 mM CAF (Gr59c,C7-GAL4/+) | <0.05 | ||||||
| Control vs 100 mM CAF (Gr59c,C7>Kir2.1) | >0.05 | ||||||
| 5F | Comparisons between tastants | Mann–Whitney U test | Water vs 10 mM denatonium (Gr59c>GCaMP6m) | 0.009 | |||
| Water vs 10 mM denatonium (C7>GCaMP6m) | 0.013 | ||||||
| 6A | Comparisons between genotypes | One-way ANOVA | F(4,35) = 10.73, P < 0.0001 | Newman–Keuls multiple comparison test | UAS-Gr59c/+ vs Gr22d>Gr59c | <0.01 | |
| Gr22d-GAL4 vs Gr22d>Gr59c | <0.01 | ||||||
| UAS-Gr59c/+ vs Gr39b>Gr59c | <0.001 | ||||||
| Gr39b-GAL4 vs Gr39b>Gr59c | <0.01 | ||||||
| 6B | Interaction between genotype and chemical | Two-way ANOVA | F(4,182) = 6.100, P = 0.0001 | Bonferroni's multiple comparison test | Control vs 10 mM denatonium (UAS-Gr59c/+) | >0.05 | |
| Control vs 10 mM denatonium (Gr22d-GAL4/+) | >0.05 | ||||||
| Control vs 10 mM denatonium (Gr22d>Gr59c) | <0.001 | ||||||
| Control vs 10 mM denatonium (Gr39b-GAL4/+) | >0.05 | ||||||
| Control vs 10 mM denatonium (Gr39b>Gr59c) | <0.001 | ||||||
| 6E | Comparisons between tastants | Mann–Whitney U test | Water vs 10 mM denatonium (Gr39b>GCaMP6m) | >0.05 | |||
| Water vs 10 mM denatonium (Gr39b>Gr59c,GCaMP6m) | <0.01 | ||||||