Primary and secondary antibodies
| Primary antibody | Vendor/ product no. | Dilution | Secondary | Vendor/product no. | Dilution |
|---|---|---|---|---|---|
| Rabbit anti-mCherry | Abcam ab167453 | 1:500 | Donkey anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, AlexaFluor 555 | ThermoFisher Scientific A-31572 | 1:500 |
| Hoechst | Fisher 33342 | 1:2000 | N/A | N/A | N/A |
| Goat Anti-GFP antibody | Abcam Ab6673 | 1:1000 | Donkey anti-Goat IgG (H+L) cross-adsorbed secondary antibody, AlexaFluor 488 | Fisher A-11055 | 1:500 |
| Mouse Anti-glial fibrillary acidic protein, clone GA5 (GFAP) | EMD Millipore MAB360 | 1:1000 | Donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 647 | Fisher A-31571 | 1:500 |
| Rat anti-Ki-67 monoclonal antibody | Invitrogen 14-5698-82 | 1:500 | AlexaFluor 647 AffiniPure donkey anti-Rat IgG (H+L) (712-605-153) | Jackson ImmunoResearch 712-605-153 | 1:500 |