Table 2

Statistics table

EYFP and tdTomato DG percentage area comparison
	TAM administration	n	Test	Comparison	Statistic	P	Significant
2E	3D Short	3	Two-way ANOVA	TAM × reporter	F_(2,6) = 8.880	0.0161	Yes*
2E	3D Long	3		TAM	F_(2,6) = 11.32	0.0092	Yes**
2E	5D	3		Reporter	F_(1,6) = 14.01	0.0096	Yes**
				Subject	F_(6,6) = 12.52	0.0036	Yes**

				EYFP		tdTomato
	Post hoc		Comparisons	Adjusted P	Significant	Adjusted P	Significant
	Tukey’s multiple comparisons		3D short vs 3D long	≥0.9999	No	0.9976	No
			3D short vs 5D	0.0009	Yes***	0.0274	Yes*
			3D long vs 5D	0.0009	Yes***	0.0308	Yes*

EYFP and tdTomato DG percentage area correlation
Figure	TAM administration	n	Test	Statistic	P	Significant
2F	All	9	Pearson's Correlation	r₍₈₎ = 0.9157	0.0005	Yes***

EYFP+ percentage area colocalization with tdTomato+ area
	TAM administration	n	Test	Statistic	P	Significant
2H	3D Short	3	One-sample t test w/ comparison to 100%	t₍₂₎ = 35.87	0.0008	Yes***
2H	3D Long	3		t₍₂₎ = 13.15	0.0057	Yes**
2H	5D	3		t₍₂₎ = 15.36	0.0042	Yes**

tdTomato+ percentage area colocalization with EYFP+ area
Figure	TAM administration	n	Test	Statistic	P	Significant
2I	3D Short	3	One-sample t test w/ comparison to 100%	t₍₂₎ = 119.7	0.0001	Yes****
2I	3D Long	3		t₍₂₎ = 10.40	0.0091	Yes**
2I	5D	3		t₍₂₎ = 11.27	0.0078	Yes**

DG percentage of NSPCs with True+ Signal
Figure	TAM administration	n	Test	Comparison	Statistic	P	Significant
4B	3D Short	3	Two-way ANOVA	True signal × Tam	F_(2,6) = 6.084	0.0360	Yes*
4B	3D Long	3		True signal	F_(1,6) = 3.215	0.1231	No
4B	5D	3		TAM	F_(2,6) = 3.201	0.1132	No
				Subject	F_(6,6) = 0.02808	0.9998	No

			Post hoc	Comparisons	Adjusted P	Significant
			Tukey’s multiple comparisons	3D short vs 3D long	0.8875	No
				3D short vs 5D	0.0225	Yes*
				3D long vs 5D	0.0516	No
DG percentage of NSPCs with True−Signal
Figure	TAM administration	n	Test	Comparison	Statistic	P	Significant
4B	3D Short	3	Two-way ANOVA	True−Signal × TAM	F_(2,6) = 6.084	0.0360	Yes*
4B	3D Long	3		True−Signal	F_(1,6) = 3.215	0.1231	No
4B	5D	3		TAM	F_(2,6) = 3.201	0.1132	No
				Subject	F_(6,6) = 0.02808	0.9998	No

			Post hoc	Comparisons	Adjusted P	Significant
			Tukey’s multiple comparisons	3D short vs 3D long	0.9389	No
				3D short vs 5D	0.0328	Yes*
				3D long vs 5D	0.0179	Yes*

DG percentage of NSPCs with True ± Signal
Figure	TAM administration	n	Test	Statistic	P	Significant
4C	3D Short	3	One-way ANOVA	F_(2,6) = 3.201	0.1132	No
4C	3D Long	3		F_(2,6) = 3.201	0.1132	No
4C	5D	3		F_(2,6) = 3.201	0.1132	No

*p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001.