Statistics for the data shown in Figures 5-Figures 8
| Figure | Statistical test | Statistics output | N |
|---|---|---|---|
| Fig. 5 | Depol + AM251: 9 neurons, 4 mice, 225 ROIs [soma (s) and non-soma (ns)] | ||
| Change in events: events (pre/post depol) × time (base, AM251, wash) | Friedman | χ2(5) = 155.838, p < 0.0005 | |
| Pre to post base | Wilcoxon signed-rank | Z = –5.418, p < 0.0005 | |
| Pre to post AM251 | Wilcoxon signed-rank | Z = –7.401, p < 0.0005 | |
| Pre to post Wash | Wilcoxon signed-rank | Z = –1.755, p = 0.079 | |
| Fig. 6A | ACPTII: 9 neurons, 3 mice | ||
| mGPSC frequency: DMSO × ACPTII | ANOVA | F(1,28) = 0.746, p = 0.395 | |
| mGPSC amplitude: DMSO × ACPTII | ANOVA | F(1,28) = 2.135, p = 0.155 | |
| mGPSC frequency: ACPTII × ACPTII + WIN | ANOVA | F(1,8) = 19.527, p = 0.002 | |
| mGPSC amplitude: ACPTII × ACPTII + WIN | ANOVA | F(1,8) = 3.206, p = 0.067 | |
| Fig. 6B | CGS: 8 neurons, 3 mice | ||
| mGPSC frequency: DMSO × CGS15943 (CGS) | ANOVA | F(1,28) = 1.539, p = 0.225 | |
| mGPSC frequency: CGS × CGS + WIN | ANOVA | F(1,7) = 1.754, p = 0.227 | |
| Fig. 6C | DPCPX: 12 neurons, 4 mice | ||
| mGPSC frequency: DMSO × DPCPX | ANOVA | F(1,31) = 0.03, p = 0.863 | |
| mGPSC amplitude: DMSO × DPCPX | ANOVA | F(1,31) = 0.032, p = 0.858 | |
| mGPSC frequency: DPCPX × DPCPX + WIN | ANOVA | F(1,11) = 0.007, p = 0.935 | |
| mGPSC amplitude: DPCPX × DPCPX + WIN | ANOVA | F(1,11) = 2.353, p = 0.153 | |
| Fig. 7 | Adenosine: 15 neurons, 4 mice | ||
| mGPSC frequency: DMSO × adenosine | ANOVA | F(1,32) = 4.144, p = 0.05 | |
| mGPSC amplitude: DMSO × adenosine | ANOVA | F(1,32) = 4.041, p = 0.053 | |
| Fig. 8 | CNO: 7 slices, 54 s, 265 ns | ||
| Increase magnitude CNO: s × ns | Mann–Whitney U | U = 2667, p = 0.95 | |
| Increase magnitude: treatment (CNO/ Thap + CNO) × ROI (s/ns) | Kruskal–Wallis | H(3) = 15.400, p = 0.001 | Thap + CNO: 3 mice, 7 s, 64 ns |
| Increase magnitude ns: CNO × Thap + CNO | Median post hoc | p = 0.022 |