Table 2.

Comparison of MEA methodology

ReferenceInstrument formatCulture modelSurface preparationCulture mediaAge (DIV)Duration (min)Detection thresholdAssay metric(s)Statistical analysis
Varghese et al. (2010)Single-wellPrimary rat (E18)DETANB12–16--MFRt test
Kuperstein et al. (2010)Single-wellPrimary mouse--8–10455 SDMFRt test
McConnell et al. (2012)Multi-wellPrimary rat (P2)LamininNB12–22338 SDMFR, active channels-
Frega et al. (2012)Single-wellPrimary rat (E18)PDL+lamininNB21–2820-MFR, burst rateKruskal–Wallis
Bateup et al. (2013a)Single-wellPrimary mousePDL+lamininNB-29 μVMFRt test, ANOVA
Biffi et al. (2013)Single-wellPrimary mousePLLNB4–35105X baselineMFR, active channelsKruskal–Wallis
Vincent et al. (2013)Single-wellPrimary rat (E18)PEI--203 SDMFRWilcoxon
Bateup et al. (2013b)Single-wellPrimary mousePDL+laminin-12–1929 μVMFRt test, ANOVA
Wainger et al. (2015)Single-wellReprogram-fibroblast neuronsPDL+lamininDMEM/F12>28-10 μVMFRMann–Whitney
Wainger et al. (2014)BothhIPS-derived neuronsPDL+laminin->24-10 μV, 5.5 SDMFRKruskal–Wallis, linear regression
Illes et al. (2014)Single-wellPrimary mousePDL+lamininDMEM/F12>21-6.2 SD of baselineMFRt test, ANOVA
Weir et al. (2014)Single-wellPrimary mouse (P1)-aCSF7–21-7SDMFR, burst rate, etc.t test, ANOVA
Charkhkar et al. (2015)Single-wellPrimary mouse (E17)PDL+lamininDMEM21205 SDMFRt test, ANOVA
Vertkin et al. (2015)Single-wellPrimary mouse (P2)--14–21--MFRt test, ANOVA
Bardy et al. (2015)Multi-wellhIPS-derived neuronsLamininBP2–21106 SDMFR-
Slomowitz et al. (2015)Single-wellPrimary mouse (P2)--15–22604–5 SDMFR-
Liu et al. (2017)Single-wellPrimary mouse---156 SDMFRt test
Alshawaf et al. (2018)Single-wellhESC-derived neurons-NB12–5655 SDMFR, active channelsKruskal–Wallis
Feng et al. (2018)Multi-wellPrimary mouse (P1)PEI+lamininNB7–2830-MFR, burst rate, etc.t test
García-León et al. (2018)Multi-wellhIPS-derived neuronsPEI+lamininDMEM/F12705-MFR, burst rate, etc.t test
Black et al. (2018)Multi-wellPrimary adult mouse DRGPEI+PDL+lamininDMEM/F123–21305.5 SDMFR, active channelsMann–Whitney
Nehme et al. (2018)Multi-wellhIPS-derived neurons-NB7–42-5 SDMFR, burst rate, etc.Kruskal–Wallis
DeRosa et al. (2018)Multi-wellhIPS-derived neuronsPEI+lamininNB>30105.25 SDMFRANOVA
Sarkar et al. (2018)Multi-wellhIPS-derived neuronsPO+lamininDMEM/F12>21105.5–6 SDMFRt test, ANOVA
Russo et al. (2018)Multi-wellhIPS-derived neuronsPO+lamininNB>3035.5 SDMFRMann–Whitney
Blake et al. (2018)Single-wellPrimary adult mouse DRGPEI+lamininNB7-5.5 SDMFRt test, ANOVA
  • Surface preparation: DETA, diethylenetriamine; PDL, poly-D-lysine; PLL, poly-L-lysine; PEI, polyethylenimine; PO; polyornithine.

  • Culture media: NB, neurobasal media; DMEM/F12, Dulbecco’s modified eagle medium: nutrient mixture F-12; aCSF, artifical CSF; BP, BrainPhys media.

  • Vacancies in the table indicate instances where the reagent or technique used was not reported or unclear in the study methods.