Experimental group parameters
| Group | Virus | Hormone replacement | n |
|---|---|---|---|
| Group 1 | ChR2 | EB+P4 | 7 |
| Group 2 | Control | EB+P4 | 6 |
| Group 3 | ChR2 | Oil | 4 |
| Group 4 | Control | Oil | 4 |
| Group 5 | ChR2 | EB+P4 | 4 |
Presented are the groups of mice used in this study, including AAV, hormone treatment, and number of mice per group. All hormones were dissolved in safflower oil and delivered subcutaneously 2 h before lights off. Mice that did not receive hormone replacement instead received an equal volume of safflower oil only.