Quantification of GFP signal intensity in the hippocampal formation
| Region | Proportions of GFP-positive cells | ||||
|---|---|---|---|---|---|
| Background | Light | Moderate | Heavy | Very heavy | |
| DG | 5.37% | 55.26% | 32.08% | 6.66% | 0.64% |
| sem | 1.79% | 6.33% | 5.05% | 2.95% | 0.24% |
| CA1 | 4.11% | 65.43% | 24.25% | 5.39% | 0.81% |
| sem | 1.33% | 7.38% | 5.43% | 3.47% | 0.69% |
| CA2 | 5.52% | 57.25% | 26.69% | 9.93% | 0.61% |
| sem | 1.55% | 7.65% | 4.96% | 3.90% | 0.26% |
| CA3 | 6.96% | 31.50% | 37.82% | 17.58% | 6.15% |
| sem | 0.92% | 4.11% | 2.05% | 2.64% | 1.45% |
The results are based on sections from three different, 3-month-old female homozygous animals. Five sections per animal were used, and quantification was performed using CellProfiler (Materials and Methods). Quantification of the GFP signal was performed by recording the intensity of the Alexa Fluor 488 in sections stained with chicken anti-GFP primary antibody and Alexa Fluor 488-conjugated anti-chicken IgY secondary antibody. Counts were based on DAPI-stained nuclei in the DG granule cell layer and in the pyramidal cell layer for CA1/CA2/CA3. All analyzed sections fell between bregma coordinates −1.355 and −2.88.