VCP overexpression does not alter the proliferative fraction of SH-SY5Y cells
| siRNA | GFP (mean ± SD) | VCP-GFP (mean ± SD) | p value |
|---|---|---|---|
| siCtrl | 0.62 ± 0.02 | 0.59 ± 0.03 | 0.16 |
| siPINK1 | 0.47 ± 0.03 | 0.46 ± 0.04 | 0.63 |
SH-SY5Y cells were co-transfected under the same conditions as in Fig. 1E. The cells were fixed after 40 h and stained for the proliferation antigen Ki-67. The proliferative fraction was calculated as the number of Ki-67 immunoreactive transfected cells divided by the total number of transfected cells (Student’s t-test, n=5). Transfection with VCP did not significantly affect cell cycle in either siCtrl or siPINK1 cells.