Statistical summary
| Identifier | Shapiro–Wilk (α = 0.05) | Test | 95% CI |
|---|---|---|---|
| a | Normal | Raldh2 mRNA puncta/mm; unpaired t test between genotypes at P2, one-tailed | –2521 to –1732 |
| b | Normal | PDGFRβ+ structures/mm2; unpaired t test between genotypes at P14, two-tailed | –42.62 to 16.82 |
| c | Normal | Laminin+ structures/mm2; unpaired t test between genotypes at P14, two-tailed | –94.79 to 21.25 |
| d | Normal | PDGFRβ+Laminin+/Laminin+ structures %, unpaired t test between genotypes at P14, two-tailed | –2.765 to 21.69 |
| e | Normal | OLIG2+ cells/mm2; one-way ANOVA with Sidak’s test between genotypes at individual time points | |
| P2 | 4.080 to 494.0 | ||
| P7 | 80.47 to 547.6 | ||
| P14 | 126.6 to 593.7 | ||
| P21 | 108.9 to 681.0 | ||
| f | Normal | OLIG2+ cells/mm2; unpaired t test within control genotype between individual time points P2 vs P21, one-tailed | –441.0 to 282.2 |
| g | Normal | OLIG2+ cells/mm2; unpaired t test within cKO genotype between individual time points P2 vs P21, one-tailed | –490.0 to 39.44 |
| h | Normal | PDGFRα+OLIG2+ cells/mm2; one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| i | Normal | PDGFRα+OLIG2+ cells/mm2; one-way ANOVA with Sidak’s test for multiple comparisons within control genotype at individual time points | |
| P2 vs P7 | –121.2 to 55.21 | ||
| P7 vs P14 | –184.2 to –16.61 | ||
| P14 vs P21 | 8.089 to 221.1 | ||
| P2 vs P21 | –224.6 to –42.19 | ||
| j | Normal | PDGFRα+OLIG2+/OLIG2+ cells/mm2; one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P2 | –26.25 to –9.080 | ||
| P7 | –21.13 to –3.288 | ||
| P14 | –20.19 to –1.376 | ||
| P21 | –12.09 to 6.720 | ||
| k | Normal | CC1+OLIG2+ cells/mm2; one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P7 | –21.38 to 87.30 | ||
| P14 | 32.72 to 141.4 | ||
| P21 | 49.38 to 158.1 | ||
| l | Normal | CC1+OLIG2+ cells/OLIG2+ cells %; one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P14 | 2.156 to 20.30 | ||
| P21 | 1.024 to 19.16 | ||
| m | Normal | PLP+ area (mm2); one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P7 | –18.20 to 107.8 | ||
| P14 | 30.06 to 138.5 | ||
| P21 | 25.44 to 139.2 | ||
| P120 | 69.15 to 211.4 | ||
| n | Normal | TUNEL+ cells/mm2; unpaired t test within control genotype between individual time points P2 vs P7, one-tailed | –41.86 to –15.94 |
| o | Normal | TUNEL+ cells/mm2; unpaired t test within cKO genotype between individual time points P2 vs P7, one-tailed | –18.13 to 4.871 |
| p | Normal | TUNEL+ cells/mm2; one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P2 | –9.109 to 16.03 | ||
| P7 | –30.05 to –7.565 | ||
| P14 | 0.9951 to 10.17 | ||
| q | Normal | IBA1+ cells/mm2 one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P7 | –46.17 to 51.34 | ||
| P14 | –92.57 to 4.941 | ||
| P21 | –37.76 to 67.56 | ||
| r | Normal | Gli1 mRNA puncta/mm2; unpaired t test between genotypes at P2, one-tailed | –15,405 to –2876 |
| s | Normal | Gli1 mRNA puncta/cell; n= number of cells, unpaired t test between genotypes at P2, one-tailed | –3.692 to –1.703 |
| t | Normal | Gli1+Olig2+ cells/mm2; unpaired t test between genotypes at P2, one-tailed | –53.01 to –33.57 |
| u | Normal | ÄGFAP+ area (mm2); one-way ANOVA with Sidak’s test for multiple comparisons between genotypes at individual time points | |
| P2 | 0.8137 to 7.880 | ||
| P7 | 4.695 to 12.44 | ||
| P14 | 6.558 to 14.30 | ||
| P21 | 3.638 to 11.38 | ||
| v | Normal | SATB2+ cells/mm2; unpaired t test between genotypes at P2, one-tailed | 159.9 to 1991 |
| w | Normal | CTIP2+ cells/mm2; unpaired t test between genotypes at P2, two-tailed | –172.6 to –68.02 |
| x | Normal | TBR1+ cells/mm2; unpaired t test between genotypes at P2, two-tailed | –1407 to –439.5 |
| y | Normal | Cortical thickness (um); unpaired t test between genotypes at P2, two-tailed | –234.6 to 26.12 |