1*F* | Normal | Student’s *t* test | *n* = 48 FLX, 42 VEH | *p* > 0.05 with Bonferroni correction |

1*J* | Normal | Student’s *t* test | *n* = 34 FLX, 25 VEH | *p* > 0.05 with Bonferroni correction |

2*I* | Non-normal | Wilcoxon rank-sum test | *n* = 30 FLX, 24 VEH | Contralateral HbO: *p* = 0.13; ipsilateral HbO: *p* = 0.0046; Contralateral GCAMP6F: *p* = 0.060; ipsilateral GCAMP6F: *p* = 0.0011 |

2*I* | Non-normal | Wilcoxon rank-sum test | *n* = 18 FLX, 11 VEH (males) | Contralateral HbO: *p* = 0.14; ipsilateral HbO: *p* = 0.010; contralateral GCAMP6F: *p* = 0.06; ipsilateral GCAMP6F: *p* = 0.038 |

2*I* | Non-normal | Wilcoxon rank-sum test | *n* = 12 FLX, 13 VEH (females) | Contralateral HbO: *p* = 0.23; ipsilateral HbO: *p* = 0.13; contralateral GCAMP6F: *p* = 0.36; ipsilateral GCAMP6F: *p* = 0.012 |

3*D* | Non-normal | Wilcoxon rank-sum test | *n* = 30 FLX, 24 VEH | Contralateral HbO: *p* = 0.050; ipsilateral HbO: *p* = 0.043; contralateral GCAMP6F: *p* = 0.028; ipsilateral GCAMP6F: *p* = 0.014 |

3-1*A*,*C* | Non-normal | Wilcoxon rank-sum test | *n* = 18 FLX, 11 VEH (males) | Contralateral HbO: *p* = 0.031; ipsilateral HbO: *p* = 0.15; contralateral GCAMP6F: *p* = 0.034; ipsilateral GCAMP6F: *p* = 0.34 |

3-1*B*,*D* | Non-normal | Wilcoxon rank-sum test | *n* = 12 FLX, 13 VEH (females) | Contralateral HbO: *p* = 0.12; ipsilateral HbO: *p* = 0.075; contralateral GCAMP6F: *p* = 0.32; ipsilateral GCAMP6F: *p* = 0.0042 |

4*C* | Normal | Student’s *t* test | *n* = 30 FLX, 24 VEH | *p* < 0.05 for regions displayed |