WT | NHE6A11S | |||||||
---|---|---|---|---|---|---|---|---|

Figure/statistical test/measure | Brain region or cells | Mean | SEM | n | Mean | SEM | n | Statistics |

Figure 2/two-tailed Student’s t test/protein expression level | ||||||||

Whole brain | 0.27 (70 kDa) 0.029 (140 kDa) | 0.02 0.003 | 6 pups | 0.27 (70 kDa) 0.023 (140 kDa) | 0.02 0.004 | 4 pups | p = 0.455 | |

Figure 3/two-tailed Student’s t test/Tfn subcellular localization | ||||||||

Cultured hippocampal neurons | 294.4 Tfn endosomes (neurites) | 41.8 | 5 pups 2723 cells 757,996 Tfn endosomes | 305.9 Tfn endosomes (neurites) | 25.4 | 5 pups 3147 cells 875,567 Tfn endosomes | p = 0.74 | |

49.2 Tfn endosomes (soma) | 4.3 | 5 pups 2723 cells 132,822 Tfn endosomes | 48.2 Tfn endosomes (soma) | 3.0 | 5 pups 3147 cells 151,240 Tfn endosomes | p = 0.84 | ||

Figure 4/two-tailed Student’s t test/endosome pH | ||||||||

Cultured hippocampal neurons | 6.48 (neurites) | 0.04 | 9 pups 4310 cells 455,578 endosomes | 6.45 (neurites) | 0.06 | 8 pups 4525 cells 683,592 endosomes | p = 0.67 | |

6.21 (soma) | 0.05 | 9 pups 4310 cells 47,809 endosomes | 6.14 (soma) | 0.06 | 8 pups 4525 cells 47,276 endosomes | p = 0.38 | ||

Figure 4/two-tailed Student’s t test/endosome area (μm^{2}) | ||||||||

Cultured hippocampal neurons | 1.034 (neurites) | 0.01 | 9 pups 4310 cells 455,578 endosomes | 1.025 (neurites) | 0.009 | 8 pups 4525 cells 683,592 endosomes | p = 0.51 | |

0.925 (soma) | 0.01 | 9 pups 4310 cells 47,809 endosomes | 0.914 (soma) | 0.02 | 8 pups 4525 cells 47,276 endosomes | p = 0.57 | ||

Figure 5/two-way ANOVA followed by Tukey’s multiple comparison test/neurite length | ||||||||

Cultured hippocampal neurons | See figure | 4 pups (WT) 3 pups (NHE6-null) | See figure | 11 pups (WT) 9 pups (NHE6A11S) | p < 0.0001 (WT vs. NHE6-null)p = 1 (WT vs. NHE6A11S) | |||

Figure 5/two-way ANOVA followed by Tukey’s multiple comparison test/neurite branch points | ||||||||

Cultured hippocampal neurons | See figure | 4 pups (WT) 3 pups (NHE6-null) | See figure | 11 pups (WT) 9 pups (NHE6A11S) | p = 0.99 (WT vs. NHE6-null)p = 1 (WT vs. NHE6A11S) | |||

Figure 6/two-tailed Student’s t test/area (cm^{2}) | ||||||||

Cerebrum | 0.97 | 0.01 | 5 pups | 0.994 | 0.005 | 4 pups | p = 0.100 | |

Cerebellum | 0.298 | 0.009 | 5 pups | 0.306 | 0.008 | 4 pups | p = 0.535 | |

Cerebellum + Midbrain | 0.348 | 0.009 | 5 pups | 0.353 | 0.007 | 4 pups | p = 0.688 | |

Whole brain | 1.31 | 0.02 | 5 pups | 1.346 | 0.007 | 4 pups | p = 0.155 | |

Figure 6/two-tailed Student’s t test/Purkinje cell density (cells/100 μm cell layer) | ||||||||

Cerebellum | 5 | 0.2 | 4 pups | 5 | 0.1 | 4 pups | p = 0.974 |

Tfn, Transferrin; WT, wild-type.