Table 3:

Summary of statistical analyses

WTNHE6A11S
Figure/statistical test/measureBrain region or cellsMeanSEMnMeanSEMnStatistics
Figure 2/two-tailed Student’s t test/protein expression level
Whole brain0.27 (70 kDa)
0.029 (140 kDa)
0.02
0.003
6 pups0.27 (70 kDa)
0.023 (140 kDa)
0.02
0.004
4 pupsp = 0.455
Figure 3/two-tailed Student’s t test/Tfn subcellular localization
Cultured hippocampal neurons294.4 Tfn endosomes (neurites)41.85 pups
2723 cells
757,996 Tfn endosomes
305.9 Tfn endosomes (neurites)25.45 pups
3147 cells
875,567 Tfn endosomes
p = 0.74
49.2 Tfn endosomes (soma)4.35 pups
2723 cells
132,822 Tfn endosomes
48.2 Tfn endosomes (soma)3.05 pups
3147 cells
151,240 Tfn endosomes
p = 0.84
Figure 4/two-tailed Student’s t test/endosome pH
Cultured hippocampal neurons6.48 (neurites)0.049 pups
4310 cells
455,578 endosomes
6.45 (neurites)0.068 pups
4525 cells
683,592 endosomes
p = 0.67
6.21 (soma)0.059 pups
4310 cells
47,809 endosomes
6.14 (soma)0.068 pups
4525 cells
47,276 endosomes
p = 0.38
Figure 4/two-tailed Student’s t test/endosome area (μm2)
Cultured hippocampal neurons1.034 (neurites)0.019 pups
4310 cells
455,578 endosomes
1.025 (neurites)0.0098 pups
4525 cells
683,592 endosomes
p = 0.51
0.925 (soma)0.019 pups
4310 cells
47,809 endosomes
0.914 (soma)0.028 pups
4525 cells
47,276 endosomes
p = 0.57
Figure 5/two-way ANOVA followed by Tukey’s multiple comparison test/neurite length
Cultured hippocampal neuronsSee figure4 pups (WT)
3 pups (NHE6-null)
See figure11 pups (WT)
9 pups (NHE6A11S)
p < 0.0001 (WT vs. NHE6-null)
p = 1 (WT vs. NHE6A11S)
Figure 5/two-way ANOVA followed by Tukey’s multiple comparison test/neurite branch points
Cultured hippocampal neuronsSee figure4 pups (WT)
3 pups (NHE6-null)
See figure11 pups (WT)
9 pups (NHE6A11S)
p = 0.99 (WT vs. NHE6-null)
p = 1 (WT vs. NHE6A11S)
Figure 6/two-tailed Student’s t test/area (cm2)
Cerebrum0.970.015 pups0.9940.0054 pupsp = 0.100
Cerebellum0.2980.0095 pups0.3060.0084 pupsp = 0.535
Cerebellum + Midbrain0.3480.0095 pups0.3530.0074 pupsp = 0.688
Whole brain1.310.025 pups1.3460.0074 pupsp = 0.155
Figure 6/two-tailed Student’s t test/Purkinje cell density (cells/100 μm cell layer)
Cerebellum50.24 pups50.14 pupsp = 0.974
  • Tfn, Transferrin; WT, wild-type.