Table 2.

List of primary and secondary antibodies used

AntibodyRR IDTarget antigenManufacturerCatalog numberHostCloneDilution
GFPAB_300798GFPAbcamab13970chickenpolyclonal1:3000 (IHC)
GFPAB_303395GFPAbcamab290rabbitpolyclonal1:1000 (WB)
ParvalbuminAB_2174013Ca+2-binding site/specifically stains the Ca+2-bound form of parvalbuminMilliporeMAB1572mousemonoclonal, IgG1, clone PARV-191:500 (IHC)
CalbindinAB_10000340Calbindin D-28kSwantCB 38rabbitpolyclonal1:1000 (IHC)
CalretininAB_10000321Calretinin humanSwant7699/3Hrabbitpolyclonal1:200 (IHC)
NeuN-biotin conjugatedAB_2298772Neuron-specific nuclear protein/FOX3/RBFOX3MilliporeMAB377Bmousemonoclonal IgG1, clone A601:500 (IHC)
Biotinylated Wisteria
Floribunda agglutinin (WFA)		N/AN-acetylgalactosamine (GalNAc)SigmaL1766N/AN/A1:100 (IHC)
GFAPAB_10013382Glial fibrillary acidic protein DakoZ0334rabbitpolyclonal1:400 (IHC)
TUBB3AB_262133Tubulin, βIIISigmaT2200rabbitpolyclonal1:500 (IHC)
SOX2N/ATranscription factor SOX-2Stem Cell Tech60055rabbitpolyclonal1:100 (IHC)
Nestin/radial glial markerAB_531887NestinDSHBRC2mousemonoclonal, IgM1:10 (IHC)
VimentinAB_531888VimentinDSHB3CB2mousemonoclonal, IgM1:10 (IHC)
Pax6AB_291612Pax6Covance Research Products PRB-278Prabbitpolyclonal1:100 (IHC)
Pax6AB_528427Pax6DSHBPAX6mousemonoclonal IgG11:10 (IHC)
CNP/RipAB_5317962',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP)DSHBRipmousemonoclonal, IgG11:100 (IHC)
Synapsin ½AB_1106784Synapsin 1/2Synaptic Systems 106 004guinea pigpolyclonal1:200 (IHC)
Ctip2AB_2064130BCL11BAbcamab18465ratpolyclonal1:500 (IHC)
Satb2AB_882455SATB2Abcamab51502mousemonoclonal, IgG11:200 (IHC)
α-TubulinAB_477579α-Tubulin SigmaT5168mousemonoclonal, IgG1, clone B-5-1-21:500 (IHC), 1:10000 (WB), 2.5μg/rxn (IP)
β-ActinAB_476697b-actinSigmaA2228mousemonoclonal, IgG1, clone AC-741:10000 (WB)
pH3AB_310177Phospho-histone H3 (Ser10)Millipore06-570rabbitpolyclonal1:500 (IHC)
Acetylated α-tubulinAB_477585α-Tubulin, acetylated (Lys-40)SigmaT6793mousemonoclonal, IgG2b, clone 6-11B-11:1000 (WB)
Anti-mono-ADP-ribose binding reagentAB_2665469Mono ADP-riboseMilliporeMABE1076E.colimonoclonal1:1000 (WB)
Secondary antibodyRR IDConjugatedManufacturerCatalog numberHost
Anti-mouse IgGAB_330924HRPCell Signaling7076Shorse
Anti-rabbit IgGAB_2099233HRPCell Signaling7074Sgoat
Anti-chicken IgYAB_2534096Alexa Fluor 488Thermo FisherA11039goat
Anti-guinea PigAB_2337438Alexa Fluor 488Jackson ImmunoResearch106-545-003goat
Anti-mouse IgGAB_2338685Cy3Jackson ImmunoResearch115-165-062goat
Anti-Mouse IgGAB_2340846Alexa Fluor 488Jackson ImmunoResearch715-545-150donkey
Anti-mouse IgM, μ chain specificAB_2340844Alexa Fluor 488Jackson ImmunoResearch715-546-020donkey
Anti-rabbit IgGAB_2307443Cy3Jackson ImmunoResearch711-165-152donkey
Anti-rabbit IgGAB_143165Alexa Fluor 488Thermo FisherA11008goat
Anti-rat IgGAB_2535794Alexa Fluor 488Thermo FisherA-21208donkey
StreptavidinAB_2337246Cy2Jackson ImmunoResearch016-220-084
StreptavidinAB_2337244Cy3Jackson ImmunoResearch016-160-084