Primer sequences and related function of assessed RNAs
| Gene | Protein function | Primer sequences (5´ to 3´) |
|---|---|---|
| b-actin | Housekeeping control | F: TTCCTTCCTGGGTATGGAAT
R: GAGGAGCAATGATCTTGATC |
| Bmal1 | Circadian clock gene, with Clock, activates Per and Cry transcription | F: AAAATGCAAGGGAGGCCCAC
R: TCTAACTTCCGGGACATCGC |
| Clock | Circadian Clock gene, with Bmal1, activates Per and Cry transcription | F: CTGCTGACAAAAGCCAAGAT
R: GACTTTCTTGAGCTTCTGGA |
| Cry1 | Circadian Clock gene, with Per, represses Bmal and Clock transcription | F: GTGGTGGCGGAAACTGCTCTC
R: ACTCTGTGCGTCCTCTTCCTGA |
| Per1 | Circadian Clock gene, with Cry, represses Bmal and Clock transcription | F: GTGCAGGCTAACCAGGAATA
R: GCGGAGAGTGTATTCAGATG |
| Per2 | Circadian Clock gene, with Cry, represses Bmal and Clock transcription | F: ACAAGCGGCTGCAGTAGTGA
R: TTCAAGGTTGCCAGCGTGCT |
| Rev-erba (Nr1d1) | Circadian Clock gene represses expression of core clock proteins | F: AGACGCTGTGCGTTTTGGAC
R: TGTGGGAACTGAGAGAAGCC |
| Cd68 | Inflammation expressed by microglia/macrophages; activation marker; cell homing and adhesion | F: CAAGCAGCACAGTGGACATTC
R: CAAGAGAAGCATGGCCCGAA |
| Iba1
(AIF1) | Inflammation expressed by all microglia/macrophages; increased with activation; binds calcium and actin | F: GGCAATGGAGATATCGATAT
R: AGAATCATTCTCAAGATGGC |
| IL-1b | Proinflammatory cytokine; secreted mainly by microglia/macrophages | F: CCTTGTGCAAGTGTCTGAAG
R: GGGCTTGGAAGCAATCCTTA |
| IL-6 | Cytokine with mixed proinflammatory and anti-inflammatory roles | F: AGAAAAGAGTTGTGCAATGGCA
R: GGCAAATTTCCTGGTTATATCC |
| Mhc II | Membrane-bound receptor; expressed by antigen-presenting cells (including microglia and macrophages); presents antigens | F: AGCACTGGGAGTTTGAAGAG
R: AAGCCATCACCTCCTGGTAT |
| Tnfa | Proinflammatory cytokine; secreted mainly by microglia/macrophages | F: CAAGGAGGAGAAGTTCCCA
R: TTGGTGGTTTGCTACGACG |
F, Forward; R, reverse.