Figure | Panel (group) | M | F | Litter | Measure | Test | Comparison | df | Statistic | p Value |
---|---|---|---|---|---|---|---|---|---|---|

Figure 1 | A–C (saline) | 12 | 11 | 9 | (A) Placental necrosis | One-way ANOVA | Effect of LPS treatment | 2, 76 | F = 8.15 | 0.0006 |

A–C (LPS 30) | 11 | 9 | 5 | One-way ANOVA | Saline vs LPS30 (Tukey's post hoc test) | 76 | q = 0.8648 | 0.8143 | ||

A–C (LPS 60) | 20 | 19 | 16 | One-way ANOVA | Saline vs LPS60 (Tukey's post hoc test) | 76 | q = 5.279 | 0.0010 | ||

One-way ANOVA | LPS30 vs LPS60 (Tukey's post hoc test) | 76 | q = 3.823 | 0.0228 | ||||||

(B, C) Placental necrosis | Two-way ANOVA | Effect of LPS treatment | 2, 73 | F = 8.16 | 0.0006 | |||||

Two-way ANOVA | LPS60 vs saline males (Tukey’s post hoc test) | 73 | q = 4.722 | 0.0161 | ||||||

Two-way ANOVA | LPS60 vs saline females (Tukey’s post hoc test) | 73 | q = 2.7 | 0.4051 | ||||||

D–F (Sal 2 h) | 65 | 57 | 18 | (D) Placental weight | Two-way ANOVA | Effect of sex E13.5 | 1, 230 | F = 17.65 | <0.0001 | |

D–F (Sal 24 h) | 76 | 66 | 22 | Two-tailed t test | LPS effect in males E13.5 | 108 | t = 2.398 | 0.0182 | ||

D–F (LPS 2 h) | 31 | 41 | 10 | Two-tailed t test | LPS effect in females E13.5 | 122 | t = 0 | >0.9999 | ||

D–F (LPS 24 h) | 48 | 44 | 18 | (E) Fetal weight | Two-way ANOVA | Effect of sex E13.5 | 1, 286 | F = 5.343 | 0.0215 | |

Two-tailed t test | LPS effect in males E13.5 | 134 | t = 1.753 | 0.0818 | ||||||

Two-tailed t test | LPS effect in females E13.5 | 152 | t = 0.0425 | 0.9662 | ||||||

(F) Ratio placenta/fetal weight | Two-way ANOVA | Effect of sex E13.5 | 1, 230 | F = 17.65 | 0.0182 | |||||

Figure 2 | A (all groups) | 6 | 6 | 3 | (A) Placenta cytokine abundance | Two-way ANOVA | Overall effect of treatment | 1, 90 | F = 124.9 | <0.0001 |

Multiple t test | Each elevated cytokine | 10 | q < 0.01 | <0.05 | ||||||

B–E (all groups) | 8 | 8 | 4 | (B) TNFα abundance | Two-way ANOVA | Effect of LPS treatment | 1, 34 | F = 14.82 | 0.0005 | |

Two-way ANOVA | Effect of LPS in males (Tukey’s post hoc test) | 34 | t = 3.302 | 0.0135 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey’s post hoc test) | 34 | t = 2.143 | 0.2143 | ||||||

(E) CXCL1 abundance | Two-way ANOVA | Effect of LPS treatment | 1, 34 | F = 655.2 | <0.0001 | |||||

Two-way ANOVA | Effect of sex | 1, 34 | F = 7.322 | 0.0106 | ||||||

Two-way ANOVA | Interaction | 1, 34 | F = 7.013 | 0.0122 | ||||||

F, G (All groups) | 3 fetuses | 3 | (F) Brain Luminex cytokines (High set) | Two-way ANOVA | Overall effect of LPS | 1, 8 | F = 34.31 | 0.0004 | ||

sex unknown | Two-way ANOVA | LPS effect on MCP3 (Tukey's post hoc test) | 8 | t = 3.614 | 0.0136 | |||||

Two-way ANOVA | LPS effect on VEGF (Tukey's post hoc test) | 8 | t = 4.669 | 0.0032 | ||||||

(G) Brain Luminex cytokines (Low set) | Two-way ANOVA | Overall effect of LPS | 1, 8 | F = 38.65 | <0.0001 | |||||

Two-way ANOVA | LPS effect on MIP1α (Tukey's post hoc test) | 8 | t = 3.128 | 0.0009 | ||||||

Two-way ANOVA | LPS effect on CXCL10 (Tukey's post hoc test) | 8 | t = 5.664 | 0.0279 | ||||||

H (saline) | 6 | 6 | 3 | (H) CXCL10 abundance (ELISA) | Two-way ANOVA | Effect of LPS treatment | 1, 20 | F = 7.532 | 0.0125 | |

H (LPS) | 6 | 6 | 3 | Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 20 | q = 4.55 | 0.0207 | ||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 20 | q = 0.9392 | 0.9093 | ||||||

Figure 3 | B, C (All groups) | 9 fetuses | >3 | (B) Umbilical perfusion | Two-tailed t test | Saline vs LPS 2 h | 35 | t = 4.096 | 0.0100 | |

sex unknown | Two-tailed t test | Saline vs LPS 24 h | 35 | t = 1.368 | 0.0900 | |||||

(C) Fetal heart rate | Two-tailed t test | Saline vs LPS 2 h | 47 | t = 5.970 | <0.0001 | |||||

Two-tailed t test | Saline vs LPS 24 h | 47 | t = 0.307 | 0.3800 | ||||||

E (saline) | 6 | 5 | 3 | (E) Hypoxia (all regions) | Two-way ANOVA | Effect of LPS in males | 1, 36 | F = 90.47 | <0.0001 | |

E (LPS) | 5 | 7 | 4 | Two-way ANOVA | Effect of LPS in females | 1, 34 | F = 9.128 | 0.0048 | ||

Two-way ANOVA | Effect of LPS in cortex (both sexes) | 1, 18 | F = 113.5 | <0.0001 | ||||||

Two-way ANOVA | Effect of sex in cortex | 1, 18 | F = 4.455 | 0.0491 | ||||||

Two-way ANOVA | Interaction cortex | 1, 18 | F = 8.186 | 0.0104 | ||||||

Two-way ANOVA | LPS M vs LPS F cortex (Tukey's post hoc test) | 18 | q = 5.232 | 0.0081 | ||||||

Two-way ANOVA | Effect of LPS in thalamus (both sexes) | 1, 17 | F = 3.844 | 0.0665 | ||||||

Two-way ANOVA | Effect of sex in thalamus | 1, 17 | F = 0.640 | 0.4348 | ||||||

Two-way ANOVA | Interaction thalamus | 1, 17 | F = 1.650 | 0.2162 | ||||||

Two-way ANOVA | Effect of LPS in LGE (both sexes) | 1, 20 | F = 3.553 | 0.0741 | ||||||

Two-way ANOVA | Effect of sex in LGE | 1, 20 | F = 4.502 | 0.0465 | ||||||

Two-way ANOVA | Interaction LGE | 1, 20 | F = 0.496 | 0.4893 | ||||||

Two-way ANOVA | Effect of LPS in MGE (both sexes) | 1, 15 | F = 2.438 | 0.1393 | ||||||

Two-way ANOVA | Effect of sex in MGE | 1, 15 | F = 0.016 | 0.9011 | ||||||

Two-way ANOVA | Interaction MGE | 1, 15 | F = 0.545 | 0.4717 | ||||||

G (saline) | 9 | 9 | 5 | (G) Metaphase cells in VZ | Two-way ANOVA | Effect of LPS treatment | 1, 34 | F = 11.07 | 0.0021 | |

G (LPS) | 11 | 9 | 6 | Two-way ANOVA | Effect of sex | 1, 34 | F = 6.092 | 0.0188 | ||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 34 | q = 3.935 | 0.0414 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 34 | q = 2.749 | 0.2295 | ||||||

(H) Total cortex area | Two-way ANOVA | Effect of sex on cortex area | 1,20 | F = 0.2975 | 0.5915 | |||||

Two-way ANOVA | Effect of LPS on cortex area | 1,20 | F = 0.0043 | 0.9486 | ||||||

Figure 4 | A (3 weeks saline) | 17 | 17 | >3 | (A) Weights 3 weeks | Two-way ANOVA | Overall effect of LPS | 1, 59 | F = 23.02 | <0.0001 |

A (3 weeks LPS) | 16 | 13 | Two-way ANOVA | Overall effect of sex | 1, 59 | F = 4.185 | 0.0453 | |||

Two-way ANOVA | Interaction | 1, 59 | F = 4.312 | 0.0422 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 59 | q = 6.69 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 59 | q = 2.801 | 0.2068 | ||||||

A (4 weeks saline) | 19 | 25 | >3 | (A) Weights 4 weeks | Two-way ANOVA | Overall effect of LPS | 1, 79 | F = 6.013 | 0.0164 | |

A (4 weeks LPS) | 23 | 13 | Two-way ANOVA | Overall effect of sex | 1, 79 | F = 19.86 | <0.0001 | |||

Two-way ANOVA | Interaction | 1, 79 | F = 8.017 | 0.0059 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 79 | q = 5.041 | 0.0034 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 79 | q = 0.3994 | 0.9921 | ||||||

A (6–8 weeks saline) | 11 | 9 | >3 | (A) Weights 6–8 weeks | Two-way ANOVA | Overall effect of LPS | 1, 39 | F = 13.33 | 0.0008 | |

A (6–8 weeks LPS) | 14 | 9 | Two-way ANOVA | Overall effect of sex | 1, 39 | F = 63.97 | <0.0001 | |||

Two-way ANOVA | Interaction | 1, 39 | F = 0.408 | 0.5283 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 39 | q = 3.277 | 0.0361 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 39 | q = 3.99 | 0.1115 | ||||||

A 11–14 weeks saline) | 11 | 10 | >3 | (A) Weights 11–14 weeks | Two-way ANOVA | Overall effect of LPS | 1, 38 | F = 33.45 | <0.0001 | |

A (11–14 weeks LPS) | 13 | 8 | Two-way ANOVA | Overall effect of sex | 1, 38 | F = 82.55 | <0.0001 | |||

Two-way ANOVA | Interaction | 1, 38 | F = 2.302 | 0.1375 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 38 | F = 3.986 | 0.0366 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 38 | F = 7.898 | <0.0001 | ||||||

A (14–17 weeks saline) | 11 | 8 | >3 | (A) Weights 14-17 weeks | Two-way ANOVA | Overall effect of LPS | 1, 36 | F = 1.031 | 0.3168 | |

A (14–17 weeks LPS ) | 14 | 7 | Two-way ANOVA | Overall effect of sex | 1, 36 | F = 63.22 | <0.0001 | |||

Two-way ANOVA | Interaction | 1, 36 | F = 0.004 | 0.9495 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 36 | q = 0.8527 | 0.9305 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 36 | q = 1.242 | 0.8161 | ||||||

(A) Weights all time points | Two-way ANOVA | LPS females vs LPS males | 1, 249 | F = 40.13 | <0.0001 | |||||

B (saline) | 9 | 6 | >3 | (B) Juvenile dyadic interactions | Two-way ANOVA | Effect of LPS (both sexes) | 1,28 | F = 2.189 | 0.1501 | |

B (LPS) | 8 | 9 | >3 | Two-way ANOVA | Interaction LPS X sex | 1,28 | F = 7.065 | 0.0128 | ||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 28 | q = 3.98 | 0.0415 | ||||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 28 | q = 1.229 | 0.8206 | ||||||

(C) Open field time in center | RM ANOVA | Effect of condition (treatment + sex) | 3, 46 | F = 2.195 | 0.3122 | |||||

C (saline) | 8 | 9 | >3 | RM ANOVA | Effect of Time | 1, 46 | F = 33.19 | <0.0001 | ||

C (LPS) | 10 | 8 | >3 | RM ANOVA | Interaction condition × time | 3, 46 | F = 2.195 | 0.1014 | ||

RM ANOVA | Saline female early vs late (Tukey's post hoc test) | 46 | t = 3.898 | 0.0013 | ||||||

RM ANOVA | LPS Female early vs late (Tukey's post hoc test) | 46 | t = 0.4042 | 0.9905 | ||||||

RM ANOVA | Saline male early vs late (Tukey's post hoc test) | 46 | t = 3.644 | 0.0027 | ||||||

RM ANOVA | LPS male early vs late (Tukey's post hoc test) | 46 | t = 4.194 | 0.0005 | ||||||

Figure 5 | B–D (saline) | 11 | 9 | 4 | (B) Socialization | Two-tailed t test | Effect of LPS in males | 23 | t = 1.856 | 0.0762 |

B–D (LPS) | 14 | 8 | 4 | Two-tailed t test | Effect of LPS in females | 23 | t = 0.4714 | 0.6442 | ||

(C-D) Social sniffing habituation | Two-way ANOVA | Effect of LPS in males | 1, 44 | F = 26.99 | 0.0073 | |||||

F–K (saline) | 10 | 11 | 5 | Two-way ANOVA | Effect of LPS in females | 1, 26 | F = 0.4238 | 0.5202 | ||

F–K (LPS) | 13 | 6 | 6 | (F) DMP latency | RM ANOVA | Effect of sex in saline | 1, 114 | F = 0.359 | 0.5503 | |

RM ANOVA | Effect of sex in LPS | 1, 102 | F = 5.832 | 0.0175 | ||||||

(G) DMP swim speed | RM ANOVA | Effect of sex in saline | 1, 114 | F = 1.259 | 0.2641 | |||||

M (saline) | 18 | 18 | >3 | RM ANOVA | Effect of sex in LPS | 1, 102 | F = 20.85 | <0.0001 | ||

M (LPS) | 13 | 6 | >3 | (H) DMP thigmotaxia | RM ANOVA | Effect of sex in saline | 1, 114 | F = 4.837 | 0.0299 | |

RM ANOVA | Effect of sex in LPS | 1, 102 | F = 17.09 | <0.0001 | ||||||

(I) DMP previous platform crossings | RM ANOVA | Effect of sex in saline | 1, 114 | F = 0.0008 | 0.9782 | |||||

RM ANOVA | Effect of sex in LPS | 1, 102 | F = 4.324 | 0.0401 | ||||||

(J) DMP platform annulus entries | RM ANOVA | Effect of sex in saline | 1, 114 | F = 0.0158 | 0.9001 | |||||

RM ANOVA | Effect of sex in LPS | 1, 102 | F = 7.396 | 0.0077 | ||||||

(L) Marble burying | Two-way ANOVA | Overall effect of LPS | 1, 51 | F = 9.939 | 0.0027 | |||||

Two-way ANOVA | Effect of LPS in males (Tukey's post hoc test) | 51 | q = 5.248 | 0.0028 | ||||||

Two-way ANOVA | Effect of LPS in females (Tukey's post hoc test) | 51 | q = 1.581 | 0.6803 | ||||||

Figure 6 | All panels and groups | 6 | 6 | >3 | (B) Cortical thickness | Two-way ANOVA | Effect of LPS | 1, 20 | F = 0.3172 | 0.5795 |

Two-way ANOVA | Effect of sex | 1, 20 | F = 0.3039 | 0.5875 | ||||||

(C–G) Density of ant. Satb2 neurons | Two-tailed t test | Totals saline male vs LPS male | 10 | t = 2.11 | 0.0611 | |||||

Two-tailed t test | Totals saline female vs LPS female | 10 | t = 1.882 | 0.0893 | ||||||

Two-way ANOVA | Overall effect of sex in saline | 1, 100 | F = 24.23 | <0.0001 | ||||||

Two-way ANOVA | Saline male vs saline female bin 2 Tukey's post hoc test) | 200 | q = 4.472 | 0.0097 | ||||||

Two-way ANOVA | Saline male vs saline female bin 3 Tukey's post hoc test) | 200 | q = 4.245 | 0.0146 | ||||||

Two-way ANOVA | Overall effect of LPS in males | 1, 100 | F = 35.98 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 2 (Tukey's post hoc test) | 200 | q = 5.037 | 0.0026 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 3 (Tukey's post hoc test) | 200 | q = 4.764 | 0.0050 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 4 (Tukey's post hoc test) | 200 | q = 3.576 | 0.0563 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 26.75 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 2 (Tukey's post hoc test) | 200 | q = 1.563 | 0.6869 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 3 (Tukey's post hoc test) | 200 | q = 1.723 | 0.6161 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 4 (Tukey's post hoc test) | 200 | q = 1.754 | 0.6018 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 24.23 | <0.0001 | ||||||

Two-way ANOVA | Baseline effect of sex, bin 2 (Tukey's post hoc test) | 200 | q = 4.472 | 0.0097 | ||||||

Two-way ANOVA | Baseline effect of sex, bin 3 (Tukey's post hoc test) | 200 | q = 4.285 | 0.0146 | ||||||

(H-L) Density of ant. PV interneurons | Two-tailed t test | Totals saline male vs LPS male | 10 | t = 2.536 | 0.0295 | |||||

Two-tailed t test | Totals saline female vs LPS female | 10 | t = 0.2372 | 0.8173 | ||||||

Two-way ANOVA | Overall effect of sex in saline | 1, 100 | F = 24.23 | <0.0001 | ||||||

Two-way ANOVA | Saline male vs saline female bin 2 Tukey's post hoc test) | 200 | q = 4.988 | 0.0029 | ||||||

Two-way ANOVA | Saline male vs saline female bin 3 Tukey's post hoc test) | 200 | q = 5.635 | 0.0005 | ||||||

Two-way ANOVA | Saline male vs saline female bin 4 Tukey's post hoc test) | 200 | q = 6.795 | <0.0001 | ||||||

Two-way ANOVA | Saline male vs saline female bin 5 Tukey's post hoc test) | 200 | q = 4.083 | 0.0222 | ||||||

Two-way ANOVA | Saline male vs saline female bin 6 Tukey's post hoc test) | 200 | q = 3.777 | 0.0406 | ||||||

Two-way ANOVA | Overall effect of LPS in males | 1, 100 | F = 18.79 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 2 (Tukey's post hoc test) | 200 | q = 5.097 | 0.0022 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 3 (Tukey's post hoc test) | 200 | q = 4.863 | 0.0039 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 4 (Tukey's post hoc test) | 200 | q = 6.159 | 0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 5 (Tukey's post hoc test) | 200 | q = 3.341 | 0.0878 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 0.1734 | 0.6730 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 2 (Tukey's post hoc test) | 200 | q = 1.133 | 0.8539 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 3 (Tukey's post hoc test) | 200 | q = 0.8094 | 0.9402 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 4 (Tukey's post hoc test) | 200 | q = 0.0665 | >0.9999 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 5 (Tukey's post hoc test) | 200 | q = 0.5111 | 0.9838 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 32.89 | <0.0001 | ||||||

Two-way ANOVA | Baseline effect of sex, bin 2 (Tukey's post hoc test) | 200 | q = 4.988 | 0.0356 | ||||||

Two-way ANOVA | Baseline effect of sex, bin 3 (Tukey's post hoc test) | 200 | q = 5.635 | 0.0107 | ||||||

Two-way ANOVA | Baseline effect of sex, bin 4 (Tukey's post hoc test) | 200 | q = 6.795 | 0.0010 | ||||||

(M–Q) Density of ant. DAPI cells | One-way ANOVA | Totals all groups | 3, 20 | F = 0.3956 | 0.7576 | |||||

Two-way ANOVA | Overall effect of LPS in males | 1, 100 | F = 9.570 | 0.0447 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 0.8449 | 0.3656 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 4.282 | 0.0761 | ||||||

Figure 7 | All panels and groups | 5 | 6 | >3 | (A–E) Density of post. Satb2 neurons | Two-tailed t test | Totals saline male vs saline female | 9 | t = 2.426 | 0.0382 |

Two-tailed t test | Totals saline male vs LPS male | 8 | t = 2.161 | 0.0626 | ||||||

Two-tailed t test | Totals saline female vs LPS female | 10 | t = 0.1802 | 0.8606 | ||||||

Two-way ANOVA | Overall effect of sex in saline | 1, 90 | F = 44.03 | <0.0001 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 2 (Tukey's post hoc test) | 180 | q = 3.842 | 0.0361 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 3 (Tukey's post hoc test) | 180 | q = 4.245 | 0.0161 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 5 (Tukey's post hoc test) | 180 | q = 5.262 | 0.0015 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 6 (Tukey's post hoc test) | 180 | q = 4.292 | 0.0146 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 7 (Tukey's post hoc test) | 180 | q = 3.868 | 0.0343 | ||||||

Two-way ANOVA | Effect of sex in saline, bin 8 (Tukey's post hoc test) | 180 | q = 3.78 | 0.0406 | ||||||

Two-way ANOVA | Overall effect of LPS in males | 1, 80 | F = 29.39 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 2 (Tukey's post hoc test) | 180 | q = 4.76 | 0.0051 | ||||||

Two-way ANOVA | Effect of LPS in males, bin #3 (Tukey's post hoc test) | 180 | q = 4.113 | 0.0211 | ||||||

Two-way ANOVA | Effect of LPS in males, bin #5 (Tukey's post hoc test) | 180 | q = 4.122 | 0.0207 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 0.2126 | 0.6457 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 26.250 | <0.0001 | ||||||

(F–J) Density of post. PV interneurons | Two-tailed t test | Totals saline male vs saline female | 9 | t = 1.392 | 0.1975 | |||||

Two-tailed t test | Totals saline male vs LPS male | 8 | t = 2.206 | 0.0584 | ||||||

Two-tailed t test | Totals saline female vs LPS female | 10 | t = 1.183 | 0.2643 | ||||||

Two-way ANOVA | Overall effect of sex in saline | 1,90 | F = 10.18 | 0.0020 | ||||||

Two-way ANOVA | Overall effect of LPS in males | 1, 80 | F = 0.0399 | <0.0001 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 5 (Tukey's post hoc test) | 180 | q = 1.276 | 0.0259 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 6.460 | 0.0126 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 5.321 | 0.0020 | ||||||

(K-O) Density of post. DAPI cells | One-way ANOVA | Totals all groups | 3,18 | F = 0.7322 | 0.5462 | |||||

Two-way ANOVA | Overall effect of sex in saline | 1, 90 | F = 0.1296 | 0.7197 | ||||||

Two-way ANOVA | Overall effect of LPS in males | 1, 100 | F = 9.570 | 0.0020 | ||||||

Two-way ANOVA | Overall effect of LPS in females | 1, 100 | F = 0.8449 | 0.3602 | ||||||

Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 4.282 | 0.7197 | ||||||

Figure 8 | Anterior cortex (A–C) | 6 | 6 | >3 | (A-C) Anterior PV/Satb2 ratio | Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 2.421 | 0.1229 |

Two-way ANOVA | Overall LPS effect in males | 1, 100 | F = 7.731 | 0.0065 | ||||||

Two-way ANOVA | Effect of LPS in males, bin 3 (Tukey's post hoc test) | 200 | q = 3.875 | 0.0336 | ||||||

Two-way ANOVA | Overall LPS effect in females | 1, 100 | F = 4.54 | 0.0356 | ||||||

Two-way ANOVA | Effect of LPS in females, bin 3 (Tukey's post hoc test) | 200 | q = 0.8335 | 0.9352 | ||||||

Posterior cortex (D–F) | 5 | 6 | >3 | (D-F) Posterior PV/Satb2 ratio | Two-way ANOVA | Baseline effect of sex in saline | 1, 100 | F = 11.36 | 0.0011 | |

Two-way ANOVA | Effect of sex in saline, bin 7 (Tukey's post hoc test) | 180 | q = 4.386 | 0.0119 | ||||||

Two-way ANOVA | Overall LPS effect in males | 1, 80 | F = 3.456 | 0.0298 | ||||||

Two-way ANOVA | Overall LPS effect in females | 1, 100 | F = 0.1391 | 0.7780 | ||||||

Extended Data Figure 1-1 | A, B (saline 2 h) | Avg. ratios | 18 | (A) Live/dead ratio | Fisher's exact test | Live/dead ratio Sal vs LPS | NA | NA | <0.0001 | |

A, B (saline 24 h) | Avg. ratios | 30 | (B) Sex ratio | Fisher's exact test | Sex Ratio Sal vs LPS | NA | NA | 0.2001 | ||

A, B (LPS 2 h) | Avg. ratios | 13 | ||||||||

A, B (LPS 24 h) | Avg. ratios | 29 | ||||||||

Extended Data Figure 4-1 | A, B (saline) | 11 | 9 | 4 | (A) Social novelty | Two-way ANOVA | Effect of LPS | 1, 38 | F = 0.0006 | 0.9807 |

A, B (LPS) | 14 | 8 | 4 | Two-way ANOVA | Effect of sex | 1, 38 | F = 0.6419 | 0.4280 | ||

(B) Buried food | Two-way ANOVA | Effect of treatment | 1, 33 | F = 1.777 | 0.1916 | |||||

Two-way ANOVA | Effect of sex | 1, 33 | F = 1.18 | 0.2852 | ||||||

C (saline) | 8 | 9 | >3 | (C) OF total ambulation | Two-way ANOVA | Effect of LPS early | 1, 46 | F = 1.226 | 0.2739 | |

C (LPS) | 10 | 8 | >3 | Two-way ANOVA | Effect of sex early | 1, 46 | F = 3.021 | 0.0889 | ||

Two-way ANOVA | Interaction early | 1, 46 | F = 0.2229 | 0.8820 | ||||||

Two-way ANOVA | Effect of LPS late | 1, 46 | F = 1.217 | 0.2758 | ||||||

Two-way ANOVA | Effect of sex late | 1, 46 | F = 1.576 | 0.2157 | ||||||

Two-way ANOVA | Interaction late | 1, 46 | F = 0.2197 | 0.6415 | ||||||

RM ANOVA | Effect of Condition (treatment + sex) | 3, 46 | F = 1.321 | 0.2790 | ||||||

RM ANOVA | Effect of time in saline F (Tukey's post hoc test) | 46 | t = 3.543 | 0.0037 | ||||||

RM ANOVA | Effect of time in LPS F (Tukey's post hoc test) | 46 | t = 1.823 | 0.2673 | ||||||

RM ANOVA | Effect of time in saline M (Tukey's post hoc test) | 46 | t = 4.127 | 0.0006 | ||||||

RM ANOVA | Effect of time in LPS M (Tukey's post hoc test) | 46 | t = 1.79 | 0.2838 |

F, Female; M, male; Avg., average; ant., anterior; post., posterior; Sal, saline; df, degrees of freedom. Bold type indicates significance.