Table 1.

Summary of cortical patterning differences after E12.5 LPS

Cell density
cells/mm2
DAPISatb2Parvalbumin
CountsANOVACountsANOVACountsANOVA
SalineLPSTreatmentInteractionSalineLPSTreatmentInteractionSalineLPSTreatmentInteraction
All layers
(bins 1–10)
M9642.329123.68p0.2370.867M2707.931537.57p<0.0010.239M1209.021041.96p0.0200.957
SD2958.591846.88F1.420.5SD1014.05632.75F88.051.33SD656.23458.25F5.650.35
Superficial
layers
(bins 1–5)
M9781.218888.16p0.18150.9483M2487.851468.41p<0.0010.1688M1218.281082.06p0.24710.8386
SD3089.571678.11F1.850.18SD1270.08792.36F29.221.71SD695.57407.22F1.380.36
Deep layers
(bins 6–10)
M9503.439359.21p0.8020.491M2928.001606.73p<0.0010.553M1199.771001.86p0.0170.820
SD2878.482008.09F0.060.09SD629.00423.83F65.500.77SD628.67509.47F6.200.38
Tbr1Cux1GABA
CountsANOVACountsANOVACountsANOVA
SalineLPSTreatmentInteractionSalineLPSTreatmentInteractionSalineLPSTreatmentInteraction
All layers
(bins 1-10)
M3307.612418.87p0.0030.695M447.82356.42p0.0340.991M425.72488.07p0.2860.924
SD2784.792082.29F9.430.71SD312.45243.69F4.620.22SD243.19343.94F1.160.42
Superficial
layers
(bins 1–5)
M3801.953106.26p0.16130.9301M621.21525.03P0.18170.9459M431.30565.78p0.13960.9094
SD3237.132388.12F2.040.21SD307.00235.83F1.850.18SD231.67405.55F2.270.26
Deep layers
(bins 6–10)
M2813.281731.47p0.0010.106M274.43187.81p0.0740.823M420.13410.37p0.8960.876
SD2201.151471.40F12.002.05SD206.0180.01F3.370.38SD258.85253.89F0.020.30
  • M, Mean. Bold type indicates significance. Two-way ANOVA for treatment and interaction effects on the cell densities in laminar bin. Significance was calculated across all bins or calculated separately for the subset of 5 bins located in superficial or deep layers of the cortex subsets of cortical projection neurons. Cux1 serves as a marker for upper layer neurons. GABA serves as a marker for cortical interneurons, and parvalbumin marks a major subset of interneurons.