Table 1.

3DMorph, Simple neurite tracer ImageJ plugin, and freehand tracing of maximum z-projection images

3DMorphSimple Neurite tracerZ-projection trace
Branch length
Cell volume
Territorial volume×
Total occupied Volume×
Ramification Index×
No. of endpoints
No. of branch points
3D analysis×
Automatic batch Processing×
User input timeFastest (min)Slowest (min–h)Intermediate (10s of min)
  • Although both 3DMorph and simple neurite tracer process 3D information, only 3DMorph offers an automatic batch processing mode to greatly decrease user input time.