p Values of the indicated statistical comparisons
| Figures | Measurement | Type of test | comparison | p Value |
|---|---|---|---|---|
| Figure 2C | EB size (EB) | Dunnett’s test | Control vs BP1 | 0.4406 |
| Control vs SCZ1 | 0.5982 | |||
| EB size (DSi-EB) | Dunnett’s test | Control vs BP1 | 0.5619 | |
| Control vs SCZ1 | 0.9784 | |||
| Figure 2D | EB form factor (EB) | Dunnett’s test | Control vs BP1 | 0.5015 |
| Control vs SCZ1 | 0.7476 | |||
| EB form factor (DSi-EB) | Dunnett’s test | Control vs BP1 | 0.0662 | |
| Control vs SCZ1 | 0.0562 | |||
| Figure 2E | PCDH15 gene expression | Dunnett’s test | Control vs BP1 | 0.3861 |
| Control vs SCZ1 | 0.3897 | |||
| Reln gene expression | Control vs BP1 | 0.7288 | ||
| Control vs SCZ1 | 0.5178 | |||
| Figure 2G | Intensity/area (βIII tubulin) | Dunnett’s test | Control vs BP1 | 0.9826 |
| Control vs SCZ1 | 0.2543 | |||
| Intensity/area (αSMA) | Dunnett’s test | Control vs BP1 | 0.9778 | |
| Control vs SCZ1 | 0.9238 | |||
| Intensity/area (AFP) | Dunnett’s test | Control vs BP1 | 0.7731 | |
| Control vs SCZ1 | 0.9345 | |||
| Figure 2J | Neurite length (day 10) | Dunnett’s test | Control vs BP1 | 0.7746 |
| Control vs SCZ1 | 0.8824 | |||
| Neurite length (day13) | Dunnett’s test | Control vs BP1 | 0.0293 | |
| Control vs SCZ1 | 0.0324 | |||
| Neurite length (day16) | Dunnett’s test | Control vs BP1 | 0.0192 | |
| Control vs SCZ1 | 0.01 | |||
| Neurite length (day22) | Dunnett’s test | Control vs BP1 | 0.0036 | |
| Control vs SCZ1 | 0.0025 | |||
| Figure 3C | No. of cells (βIII tubulin+/HO+) | Tukey’s test | 1210B2 vs 201B7 | 0.9993 |
| 1210B2 vs BP1-1 | 1 | |||
| 1210B2 vs BP1-2 | 1 | |||
| 1210B2 vs BP2-1 | 0.0218 | |||
| 1210B2 vs SCZ1-1 | 0.5216 | |||
| 1210B2 vs SCZ1-2 | 0.875 | |||
| 201B7 vs BP1-1 | 0.9975 | |||
| 201B7 vs BP1-2 | 0.9989 | |||
| 201B7 vs BP2-1 | 0.0036 | |||
| 201B7 vs SCZ1-1 | 0.2079 | |||
| 201B7 vs SCZ1-2 | 0.5352 | |||
| BP1-1 vs BP1-2 | 1 | |||
| BP1-1 vs BP2-1 | 0.0077 | |||
| BP1-1 vs SCZ1-1 | 0.4016 | |||
| BP1-1 vs SCZ1-2 | 0.8143 | |||
| BP1-2 vs BP2-1 | 0.0042 | |||
| BP1-2 vs SCZ1-1 | 0.3116 | |||
| BP1-2 vs SCZ1-2 | 0.7283 | |||
| BP2-1 vs SCZ1-1 | 0.5238 | |||
| BP2-1 vs SCZ1-2 | 0.1306 | |||
| SCZ1-1 vs SCZ1-2 | 0.9847 | |||
| No. of cells (MAP2+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 1 | |
| 1210B2 vs BP1-1 | 0.6317 | |||
| 1210B2 vs BP1-2 | 0.9984 | |||
| 1210B2 vs BP2-1 | 0.6973 | |||
| 1210B2 vs SCZ1-1 | 0.9988 | |||
| 1210B2 vs SCZ1-2 | 0.9994 | |||
| 201B7 vs BP1-1 | 0.7153 | |||
| 201B7 vs BP1-2 | 1 | |||
| 201B7 vs BP2-1 | 0.7806 | |||
| 201B7 vs SCZ1-1 | 1 | |||
| 201B7 vs SCZ1-2 | 1 | |||
| BP1-1 vs BP1-2 | 0.7807 | |||
| BP1-1 vs BP2-1 | 1 | |||
| BP1-1 vs SCZ1-1 | 0.8539 | |||
| BP1-1 vs SCZ1-2 | 0.7703 | |||
| BP1-2 vs BP2-1 | 0.8448 | |||
| BP1-2 vs SCZ1-1 | 1 | |||
| BP1-2 vs SCZ1-2 | 1 | |||
| BP2-1 vs SCZ1-1 | 0.8955 | |||
| BP2-1 vs SCZ1-2 | 0.8325 | |||
| SCZ1-1 vs SCZ1-2 | 1 | |||
| No. of cells (VGluT2+βIII tubulin+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9629 | |
| 1210B2 vs BP1-1 | 0.2892 | |||
| 1210B2 vs BP1-2 | 0.9885 | |||
| 1210B2 vs BP2-1 | 0.8416 | |||
| 1210B2 vs SCZ1-1 | 0.6553 | |||
| 1210B2 vs SCZ1-2 | 0.9756 | |||
| 201B7 vs BP1-1 | 0.7818 | |||
| 201B7 vs BP1-2 | 0.9999 | |||
| 201B7 vs BP2-1 | 0.9996 | |||
| 201B7 vs SCZ1-1 | 0.9859 | |||
| 201B7 vs SCZ1-2 | 1 | |||
| BP1-1 vs BP1-2 | 0.5003 | |||
| BP1-1 vs BP2-1 | 0.9484 | |||
| BP1-1 vs SCZ1-1 | 0.9956 | |||
| BP1-1 vs SCZ1-2 | 0.6512 | |||
| BP1-2 vs BP2-1 | 0.9885 | |||
| BP1-2 vs SCZ1-1 | 0.9093 | |||
| BP1-2 vs SCZ1-2 | 1 | |||
| BP2-1 vs SCZ1-1 | 0.9998 | |||
| BP2-1 vs SCZ1-2 | 0.9975 | |||
| SCZ1-1 vs SCZ1-2 | 0.9616 | |||
| Figure 3D | PCDH15 gene expression (iPS) | Dunnett’s test | Control vs BP | 0.9527 |
| Control vs SCZ | 0.9354 | |||
| PCDH15 gene expression (neuron) | Dunnett’s test | Control vs BP | 0.2862 | |
| Control vs SCZ | 0.4102 | |||
| RELN gene expression (iPS) | Dunnett’s test | Control vs BP | 0.1826 | |
| Control vs SCZ | 0.3746 | |||
| RELN gene expression (neuron) | Dunnett’s test | Control vs BP | 0.6918 | |
| Control vs SCZ | 0.9941 | |||
| Figure 3G | No. of cells (βIII tubulin+/HO+) | Tukey’s test | 1210B2 vs 201B7 | 0.98 |
| 1210B2 vs BP1-1 | 0.9914 | |||
| 1210B2 vs BP1-2 | 0.9863 | |||
| 1210B2 vs BP2-1 | 0.4556 | |||
| 1210B2 vs SCZ1-1 | 1 | |||
| 1210B2 vs SCZ1-2 | 0.9992 | |||
| 201B7 vs BP1-1 | 0.7241 | |||
| 201B7 vs BP1-2 | 1 | |||
| 201B7 vs BP2-1 | 0.1395 | |||
| 201B7 vs SCZ1-1 | 0.9894 | |||
| 201B7 vs SCZ1-2 | 0.9993 | |||
| BP1-1 vs BP1-2 | 0.7192 | |||
| BP1-1 vs BP2-1 | 0.7873 | |||
| BP1-1 vs SCZ1-1 | 0.9536 | |||
| BP1-1 vs SCZ1-2 | 0.8677 | |||
| BP1-2 vs BP2-1 | 0.1102 | |||
| BP1-2 vs SCZ1-1 | 0.9937 | |||
| BP1-2 vs SCZ1-2 | 0.9998 | |||
| BP2-1 vs SCZ1-1 | 0.2366 | |||
| BP2-1 vs SCZ1-2 | 0.1646 | |||
| SCZ1-1 vs SCZ1-2 | 0.9999 | |||
| No. of cells (MAP2+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9997 | |
| 1210B2 vs BP1-1 | 0.9897 | |||
| 1210B2 vs BP1-2 | 0.9829 | |||
| 1210B2 vs BP2-1 | 0.5806 | |||
| 1210B2 vs SCZ1-1 | 0.9812 | |||
| 1210B2 vs SCZ1-2 | 0.9842 | |||
| 201B7 vs BP1-1 | 1 | |||
| 201B7 vs BP1-2 | 0.9998 | |||
| 201B7 vs BP2-1 | 0.8884 | |||
| 201B7 vs SCZ1-1 | 0.9037 | |||
| 201B7 vs SCZ1-2 | 0.9149 | |||
| BP1-1 vs BP1-2 | 1 | |||
| BP1-1 vs BP2-1 | 0.902 | |||
| BP1-1 vs SCZ1-1 | 0.6069 | |||
| BP1-1 vs SCZ1-2 | 0.6541 | |||
| BP1-2 vs BP2-1 | 0.9712 | |||
| BP1-2 vs SCZ1-1 | 0.6265 | |||
| BP1-2 vs SCZ1-2 | 0.6636 | |||
| BP2-1 vs SCZ1-1 | 0.0941 | |||
| BP2-1 vs SCZ1-2 | 01225 | |||
| SCZ1-1 vs SCZ1-2 | 1 | |||
| No. of cells (GABA+βIII tubulin+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9415 | |
| 1210B2 vs BP1-1 | 0.2961 | |||
| 1210B2 vs BP1-2 | 0.6938 | |||
| 1210B2 vs BP2-1 | 0.2707 | |||
| 1210B2 vs SCZ1-1 | 0.343 | |||
| 1210B2 vs SCZ1-2 | 0.8786 | |||
| 201B7 vs BP1-1 | 0.9599 | |||
| 201B7 vs BP1-2 | 0.9994 | |||
| 201B7 vs BP2-1 | 0.949 | |||
| 201B7 vs SCZ1-1 | 0.9747 | |||
| 201B7 vs SCZ1-2 | 1 | |||
| BP1-1 vs BP1-2 | 0.9981 | |||
| BP1-1 vs BP2-1 | 1 | |||
| BP1-1 vs SCZ1-1 | 1 | |||
| BP1-1 vs SCZ1-2 | 0.9345 | |||
| BP1-2 vs BP2-1 | 0.9967 | |||
| BP1-2 vs SCZ1-1 | 0.9994 | |||
| BP1-2 vs SCZ1-2 | 0.9993 | |||
| BP2-1 vs SCZ1-1 | 1 | |||
| BP2-1 vs SCZ1-2 | 0.9169 | |||
| SCZ1-1 vs SCZ1-2 | 0.9587 | |||
| Figure 3H | PCDH15 gene expression (iPS) | Dunnett’s test | Control vs BP | 0.2334 |
| Control vs SCZ1 | 0.8567 | |||
| PCDH15 gene expression (neuron) | Dunnett’s test | Control vs BP | 0.3458 | |
| Control vs SCZ | 0.5146 | |||
| RELN gene expression (iPS) | Dunnett’s test | Control vs BP | 0.999 | |
| Control vs SCZ | 0.9874 | |||
| RELN gene expression (neuron) | Dunnett’s test | Control vs BP | 0.081 | |
| Control vs SCZ | 0.0663 | |||
| Figure 5D | Dendrite length | Dunnett’s test | Control vs BP | 0.0001 |
| Control vs SCZ | 0.0002 | |||
| Figure 5E | Homer I puncta No. | Dunnett’s test | Control vs BP | 0.0093 |
| Control vs SCZ | 0.009 | |||
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0067 | |
| Control vs SCZ | 0.0014 | |||
| Homer I-Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0182 | |
| Control vs SCZ | 0.0117 | |||
| Figure 5H | Dendrite length | Dunnett’s test | Control vs BP | 0.004 |
| Control vs SCZ | 0.0016 | |||
| Figure 5I | Gephyrin puncta No. | Dunnett’s test | Control vs BP | 0.0065 |
| Control vs SCZ | 0.0048 | |||
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0173 | |
| Control vs SCZ | 0.0099 | |||
| Gephyrin-Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0044 | |
| Control vs SCZ | 0.0042 | |||
| Figure 6E | Gene expression of PCDH15 | Student’s t test | Control 1 vs PCDH15del (glutamatergic neurons) | 0.1757 |
| Control 1 vs PCDH15del (GABAergic neurons) | 0.01 | |||
| Figure 6G | Gene expression of RELN | Student’s t test | Control 2 vs RELNdel (glutamatergic neurons) | <0.0001 |
| Control 2 vs RELNdel (GABAergic neurons) | 0.0014 | |||
| Figure 7B | No. of cells (βIII tubulin+/HO+) | Student’s t test | Control 1 vs PCDH15del | 0.1781 |
| Control 1 vs RELNdel | 0.2969 | |||
| No. of cells (MAP2+/βIII tubulin+) | Student’s t test | Control 1 vs PCDH15del | 0.0003 | |
| Control 1 vs RELNdel | 0.2568 | |||
| No. of cells (VGluT2+βIII tubulin+/βIII tubulin+) | Student’s t test | Control 1 vs PCDH15del | 0.0098 | |
| Control 1 vs RELNdel | 0.2754 | |||
| Figure 7C | Dendrite length | Student’s t test | Control 1 vs PCDH15del | 0.0418 |
| Control 1 vs RELNdel | 0.063 | |||
| Figure 7D | Homer I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.0282 |
| Control 1 vs RELNdel | 0.0209 | |||
| Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.2101 | |
| Control 1 vs RELNdel | 0.3278 | |||
| Homer I-Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.982 | |
| Control 1 vs RELNdel | 0.7786 | |||
| Figure 7F | No. of cells (βIII tubulin+/HO+) | Control 1 vs PCDH15del | 0.1441 | |
| Control 1 vs RELNdel | 0.1954 | |||
| No. of cells (MAP2+/βIII tubulin+) | Control 1 vs PCDH15del | 0.0281 | ||
| Control 1 vs RELNdel | 0.4007 | |||
| No. of cells (GABA+βIII tubulin+/βIII tubulin+) | Control 1 vs PCDH15del | 0.1269 | ||
| Control 1 vs RELNdel | 0.66 | |||
| Figure 7G | Dendrite length | Student’s t test | Control 1 vs PCDH15del | 0.0055 |
| Control 1 vs RELNdel | 0.9907 | |||
| Figure 7H | Gephyrin puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.553 |
| Control 1 vs RELNdel | 0.0452 | |||
| Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.2637 | |
| Control 1 vs RELNdel | 0.0431 | |||
| Gephyrin-Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.5136 | |
| Control 1 vs RELNdel | 0.3123 | |||
| Figure 8B | Dendrite length (Glutamatergic neurons) | Dunnett’s test | Control vs BP | 0.0001 |
| Control vs SCZ | 0.0002 | |||
| Homer I puncta No. | Dunnett’s test | Control vs BP | 0.0003 | |
| Control vs SCZ | 0.001 | |||
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0011 | |
| Control vs SCZ | 0.0012 | |||
| Homer I-Synapsin I puncta No. | Dunnett’s test | Control vs BP | <0.0001 | |
| Control vs SCZ | <0.0001 | |||
| Dendrite length (GABAergic neurons) | Dunnett’s test | Control vs BP | 0.0021 | |
| Control vs SCZ | 0.0027 | |||
| Gephyrin puncta No. | Dunnett’s test | Control vs BP | 0.0002 | |
| Control vs SCZ | 0.0011 | |||
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.1728 | |
| Control vs SCZ | 0.1023 | |||
| Gephyrin-Synapsin I puncta No. | Dunnett’s test | Control vs BP | <0.0001 | |
| Control vs SCZ | <0.0001 | |||
| Figure 8E | Spike frequency | Dunnett’s test | Control vs BP (day28) | 0.8839 |
| Control vs SCZ (day28) | 0.924 | |||
| Control vs BP (day42) | 0.7486 | |||
| Control vs SCZ (day42) | 0.9812 | |||
| Paired t test | day28 vs day42 | 0.0104 | ||
| Figure 8F | Spike frequency | Dunnett’s test | Control 1 vs PCDH15del (day28) | 0.6129 |
| Control 2 vs RELNdel (day28) | 0.7575 | |||
| Control 1 vs PCDH15del (day42) | 0.2678 | |||
| Control 2 vs RELNdel (day42) | 0.9187 | |||
| Paired t test | day28 vs day42 | 0.0483 | ||
| Figure 8G | Spike number ratio (CNQX) | Dunnett’s test | Control vs BP | 0.0351 |
| Control vs SCZ | 0.0479 | |||
| Spike number ratio (AP-5) | Control vs BP | 0.1922 | ||
| Control vs SCZ | 0.1165 | |||
| Spike number ratio (GABA) | Control vs BP | 0.1204 | ||
| Control vs SCZ | 0.0071 | |||
| Figure 8I | ΔFmax | Dunnett’s test | 1210B2 vs BP1-1 | 0.9215 |
| 1210B2 vs BP1-2 | 0.9233 | |||
| 1210B2 vs BP2-1 | 1 | |||
| 1210B2 vs SCZ1-1 | 0.9999 | |||
| 1210B2 vs SCZ1-2 | 0.9997 | |||
| Spike frequency | Dunnett’s test | 1210B2 vs BP1-1 | 0.2334 | |
| 1210B2 vs BP1-2 | 0.7435 | |||
| 1210B2 vs BP2-1 | 0.9984 | |||
| 1210B2 vs SCZ1-1 | 0.9985 | |||
| 1210B2 vs SCZ1-2 | 1 | |||
| Figure 8J | ΔFmax | Student’s t test | Control 1 vs PCDH15del | 0.7346 |
| Control 1 vs RELNdel | 0.5067 | |||
| Spike frequency | Student’s t test | Control 1 vs PCDH15del | 0.0795 | |
| Control 1 vs RELNdel | 0.3771 | |||
p Values <0.05 were considered to be statistically significant in this study.