Used primers for generating isogenic PCDH15 deletion iPSCs
| Targets | Primers | Sequence (5´ → 3´) |
|---|---|---|
| sgRNAs construction | sgRNA#1-Fw | GAGACCACTTGGATCCGGACGGCAATCACGAGTGTTGTTTTAGAGCTAGAAATAGCA |
| sgRNA#2-Fw | GAGACCACTTGGATCCGTCGCCTCTCATTCAGATTTGTTTTAGAGCTAGAAATAGCA | |
| sgRNA#3-Fw | GAGACCACTTGGATCCGTGGCAGCTTGATAAGTGAGGTTTTAGAGCTAGAAATAGCA | |
| sgRNA#4-Fw | GAGACCACTTGGATCCGCGCCTCTCATTCAGATTTTGTTTTAGAGCTAGAAATAGCA | |
| sgRNA#5-Fw | GAGACCACTTGGATCCGCTCATTCAGATTTTGGGCAGTTTTAGAGCTAGAAATAGCA | |
| sgRNA#universal-Rv | GCCCGGGTTTGAATTCAAAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA | |
| T7E1 assay | Fw | CTCAGTTTACATCCTGACTCAACCAC |
| Rv | CCTTCAAACGGCCAAACATAATCTCC |
Sequence information of the primers for sgRNAs construction and T7E1 assay. Fw, Forward primer; Rv, reverse primer. Also see Materials and Methods.