Training steps, optogenetic suppression
Training step | Description | Advancement criterion |
---|---|---|
1. Surgery | Fiber implantation | 1 d of water restriction after recovery |
2. Free drinks | Ports give water, no stimulus | Trial rate (cannot trigger the same port repeatedly) |
3. Requests rewarded | Rewards given for center-port trial requests and for correct responses | Trial rate (7 trials in 30 s) |
4. Only correct responses rewarded | Rewards only given for correct responses | 400 trials completed |
5. Longer penalty | Increased timeout for incorrect responses, no correction trials | Sustained performance > ∼85% |
6. Optogenetic suppression | Laser on for 10% of trials | N/A |