Table 1.

Training steps, optogenetic suppression

Training stepDescriptionAdvancement criterion
1. SurgeryFiber implantation1 d of water restriction after recovery
2. Free drinksPorts give water, no stimulusTrial rate (cannot trigger the same port repeatedly)
3. Requests rewardedRewards given for center-port trial requests and for correct responsesTrial rate (7 trials in 30 s)
4. Only correct responses rewardedRewards only given for correct responses400 trials completed
5. Longer penaltyIncreased timeout for incorrect responses, no correction trialsSustained performance > ∼85%
6. Optogenetic suppressionLaser on for 10% of trialsN/A