Statistical analyses
| Data structure | Type of test | Condition | Power | |
|---|---|---|---|---|
| Fig. 3B | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | *p = 0.0393 |
| Four weeks:organoids vs four weeks:NPCs | n.s., p = 0.7729 | |||
| Fig. 3D | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.1836 |
| Four weeks:organoids vs four weeks:NPCs | n.s., p = 0.9634 | |||
| Fig. 5B, left | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.1059 |
| Four weeks:organoids vs four weeks:NPCs | *p = 0.0293 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.4072 | |||
| Fig. 5B, right | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.6113 |
| Four weeks:organoids vs four weeks:NPCs | n.s., p = 0.7485 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.2955 | |||
| Fig. 6B, top | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | **p = 0.0014 |
| Four weeks:organoids vs four weeks:NPCs | *p = 0.0108 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.9757 | |||
| Fig. 6B, bottom | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:NPC vs two weeks:Organoid | *p = 0.0385 |
| Four weeks:NPC vs four weeks:Organoid | n.s., p = 0.2141 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.998 | |||
| Fig. 6D, top | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.1664 |
| Four weeks:organoids vs four weeks:NPCs | n.s., p = 0.7125 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.3625 | |||
| Fig. 6D, bottom | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.9555 |
| Four weeks:organoids vs four weeks:NPCs | n.s., p = 0.9037 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.2857 | |||
| Fig. 8B | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.2590 |
| Four weeks:organoids vs four weeks:NPCs | *p = 0.0311 | |||
| Four weeks:organoids vs two weeks:organoids | n.s., p = 0.1724 | |||
| Fig. 8D | Dataset with more than two groups | Two-way ANOVA - Tukey post hoc test | Two weeks:organoids vs two weeks:NPCs | n.s., p = 0.6897 |
| Four weeks:organoids vs four weeks:NPCs | ***p = 0.0006 | |||
| Four weeks:organoids vs two weeks:organoids | **p = 0.0041 | |||
| Fig. 8F | Dataset with two groups | Shapiro–Wilk followed by a Student’s t test | Two weeks:organoids vs four weeks:organoids | n.s., p = 0.3437 |