Statistical table
| Figure | Description | Type of test | Power (n) | p value | |
|---|---|---|---|---|---|
| 1C | Heatmap of cytokine expression in the brain and heart following ischemia, compared to naïve control | One-way ANOVA | 5–11 | ||
| Brain 24 h | <0.0001 (G-CSF, KC, MCP-1, MIP-2), 0.0372 (GM-CSF), 0.0396 (IL-5), 0.028 (IL-6), 0.0204 (TNF-α) | ||||
| Brain 1 week | 0.0117 (IP-10), <0.0001 (MCP-1, MIP-1α, MIP-1β, TNF-α) | ||||
| Brain 8 weeks | <0.0001 (IP-10, RANTES, IL-4), 0.0013 (KC), 0.0136 (MCP-1), 0.003 (MIP-1α), 0.0024 (MIP-1β), 0.0215 (MIP-2), 0.0007 (IL-9), 0.0012 (IL-12 p70) | ||||
| Heart 24 h | <0.0001 (G-CSF, GM-CSF, IL-1β, IL-5, IL-6, IP-10, KC, MCP-1, MIP-1α, MIP-1β, MIP-2, IL-9) | ||||
| Heart 1 week | <0.0001 (IP-10, RANTES) | ||||
| 2B | Quantification of immune cells present in the brain infarct at four and eight weeks following stroke | Unpaired t test | 3 | 0.0011 (CD3e), 0.0063 (B220) | |
| 3H | Quantification of cholesterol crystals within infarcted brain | One-way ANOVA | 3 | **p < 0.01, ****p < 0.0001 | |
| 3J | Quantification of cholesterol crystals purified from seven-week brain infarct | Two-way ANOVA Sidak’s multiple comparisons | 3 | **p < 0.01 | |
| 4A | OPN expression in the brain compared to the heart following ischemia (normalized to naïve) | Multiple t tests Holm–Sidak post hoc | 4 | *p < 0.05 | |
| 4C | IL-18 expression in the brain compared to the heart following ischemia, compared to naïve control | Multiple t tests | 4 | 0.0005 (one week), 0.03 (four weeks), <0.0001 (eight weeks) | |
| 4D | MMP-2, MMP-3, and MMP-8 expression in the brain compared to the heart following ischemia, compared to naïve control | Multiple t tests | 5 | ||
| MMP-2 | <0.0001 (24 h), 0.0144 (one week), 0.00113 (eight weeks) | ||||
| MMP-3 | 0.0008 (24 h), 0.00363 (one week), 0.01626 (four weeks), <0.0001 (eight weeks) | ||||
| MMP-8 | <0.0001 (one week), 0.01906 (four weeks), 0.01977 (eight weeks) | ||||
| 5B | Quantification of cholesterol crystals in infarcted brain of BALB/c mice | Unpaired t test | 4–5 | 0.0242 | |
| 5C | MMP-2, MMP-3, and MMP-8 expression in infarcted brain of BALB/c mice | Multiple t tests | 3–6 | 0.0005 (MMP-2), 0.00333 (MMP-3), 0.00157 (MMP-8) | |
| 6B | Quantification of immune cells present in the brain infarct at eight and 24 weeks following stroke | Unpaired t test | 3–6 | ****p < 0.0001 | |
| 6C | Quantification of collagen deposition in the infarct at four, eight, and 24 weeks following stroke | One-way ANOVA | 3–7 | ****p < 0.0001 | |
| 6D | Cytokine expression in naïve, eight-week, and 24-week post-stroke brain, compared to 24-week sham | Multiple t tests | 5–6 | 0.00637 (G-CSF), 0.0000456 (IL-4), 0.000149 (IL-5), 0.000737 (IL-6), 0.048 (IL-9), 0.00753 (IP-10), 0.00036 (KC), <0.0001 (MCP-1), 0.00694 (MIP-1α), 0.00424 (MIP-1β), 0.00275 (MIP-2), 0.00017 (RANTES) | |
| 6E | MMP-2, MMP-3, and MMP-8 expression in infarcted brain at multiple timepoints, compared to naïve control | One-way ANOVA | 5–6 | *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 | |
| 7B | Infarct volumes 24 hrs following stroke in NLRP3-/-, CD36, and OPN-/- mice | One-way ANOVA | 5 | No differences | |
| 7C | Heatmap of cytokine expression in the infarcted brain of NLRP3-/-, CD36-/-, and OPN-/- mice, compared to their WT counterpart | Unpaired t test | 4 | ||
| NLRP3-/- | 0.05 (IL-1α), 0.0418 (IL-1β) | ||||
| CD36-/- | 0.0257 (TNF-α) | ||||
| OPN-/- | 0.0087 (G-CSF), 0.0315 (IL-12 p70), 0.0289 (IP-10), 0.0295 (MCP-1), 0.019 (MIP-1α), 0.0049 (MIP-1β), 0.0077 (RANTES), 0.0240 (TNF-α) | ||||
| 7D | MMP-2, MMP-3, and MMP-8 expression in infarcted brain of NLRP3-/-, CD36-/-, and OPN-/- mice, compared to their WT counterpart | Multiple t tests | 4 | 0.0189 (OPN MMP-2), 0.042 (OPN MMP-8) | |
| 8A | Cytokine expression in the infarct in WT and OPN-/- mice at 24 h | Multiple t tests | 5 | 0.03642 (IL-5), 0.03588 (IL-6) | |
| 8B | Cytokine expression in the infarct in WT and OPN-/- mice at one week | Multiple t tests | 5–8 | 0.003066 (GM-CSF), 0.02468 (IL-1β), 0.035148 (IL-5), 0.002543 (MIP-1α), 0.00165 (MIP-1β) | |
| 8C | Cytokine expression in the infarct in WT and OPN-/- mice at seven weeks | Multiple t tests | 4 | 0.02627 (G-CSF), 0.0315 (IL-12 p70), 0.0393 (IP-10), 0.04993 (MCP-1), 0.02156 (MIP-1α), 0.00774 (MIP-1β), 0.01515 (RANTES), 0.03954 (TNF-α) | |
| 8D | Comparison of the cytokine milieu in the infarct of WT and OPN-/- mice at 24 h, one week, and seven weeks following stroke | Two-way ANOVA | 4–5 | ****p < 0.0001 | |
| 9D | Quantification of cholesterol crystals in infarct seven weeks post stroke in WT and OPN-/- mice | Unpaired t test | 4–7 | 0.0412 | |
| 9E | Ladder rung test scoring of WT and OPN-/- mice following stroke | Multiple t tests | 11–12 | 0.0041 (**), 0.0405 (*) | |
| 9H | Percent of NeuN immunoreactivity of ipsilateral/contralateral striatum in WT and OPN-/- mice seven weeks following stroke | Unpaired t test | 5–7 | 0.0206 | |