Table 2.

Statistical analyses for data

Set of data		Type of statistical analysis	Results of statistical analysis
Figure 1
C: DA levels in the NAc with cocaine infusion into the NAc
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,120) = 49.4312	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,120) = 9.4748	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,120) = 4.1100	p = 0.0001
C: DA levels in the NAc with cocaine infusion into the NAc (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 1.2	p = 0.2351
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 5.5	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 6.54	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 7.04	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 6.53	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 5.23	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 4.39	p < 0.0001
C: DA levels in the NAc with cocaine infusion into the NAc (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = -0.69	p = 0.4942
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 0.3	p = 0.7639
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 1.54	p = 0.1286
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 1.79	p = 0.0783
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 2.19	p = 0.0326
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 3.78	p = 0.0004
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 2.86	p = 0.0059
D: DA levels in the NAc with exposure to palatable food
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,120) = 37.1184	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,120) = 3.4984	p = 0.0007
	Group-time interaction	Two-way ANOVA	F_(9,120) = 2.3706	p = 0.0167
D: DA levels in the NAc with exposure to palatable food (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 2.08	p = 0.0421
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 4.42	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 5.64	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 4.7	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 3.15	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 2.71	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 2.23	p < 0.0001
D: DA levels in the NAc with exposure to palatable food (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 1.15	p = 0.2544
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 2.03	p = 0.0475
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 1.72	p = 0.0911
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = -0.07	p = 0.9414
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = -1.7	p = 0.095
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = -0.72	p = 0.4769
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = -0.74	p = 0.464
E: DA levels in the NAc with exposure to female mice
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,80) = 39.2674	p < 0.0001
	Time effect	Two-way ANOVA	F_(7,80) = 7.0594	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(7,80) = 3.7936	p = 0.0013
E: DA levels in the NAc with exposure to female mice (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₃₅₎ = 5.69	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₃₅₎ = 8.36	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₃₅₎ = 5.29	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₃₅₎ = 3.39	p = 0.0017
	Basal vs 100 min	Mixed linear models	t₍₃₅₎ = 3.06	p = 0.0042

E: DA levels in the NAc with exposure to female mice (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₃₅₎ = 0.33	p = 0.7429
	Basal vs 40 min	Mixed linear models	t₍₃₅₎ = 2.21	p = 0.0335
	Basal vs 60 min	Mixed linear models	t₍₃₅₎ = 0.46	p = 0.6483
	Basal vs 80 min	Mixed linear models	t₍₃₅₎ = 0.17	p = 0.865
	Basal vs 100 min	Mixed linear models	t₍₃₅₎ = -1.9	p = 0.5526
F: DA levels in the PFC with cocaine infusion
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,120) = 0.00970	p = 0.7560
	Time effect	Two-way ANOVA	F_(9,120) = 8.8283	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,120) = 0.00895	p = 0.9997
F: DA levels in the PFC with cocaine infusion into the PFC (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 0.9	p = 0.3719
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 3.04	p = 0.0037
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 4.29	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 5.3	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 5.98	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 4.86	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 4.23	p < 0.0001
F: DA levels in the PFC with cocaine infusion into the PFC (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 1.06	p = 0.2933
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 3.26	p = 0.0019
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 4.61	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 5.4	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 4.94	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 5.18	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 5.09	p < 0.0001
G: DA levels in the PFC with exposure to palatable food
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,120) = 0.0733	p = 0.7870
	Time effect	Two-way ANOVA	F_(9,120) = 11.4806	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,120) = 0.1100	p = 0.9994
G: DA levels in the PFC with exposure to palatable food (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 4.23	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 5.92	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 4.67	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 4.22	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 3.99	p = 0.0002
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 2.54	p = 0.0139
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 1.9	p = 0.0631
G: DA levels in the PFC with exposure to palatable food (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 4.55	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 5.86	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 5.41	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 4.84	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 4.42	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 3.37	p = 0.0014
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 3.14	p = 0.0028
H: DA levels in the PFC with exposure to female mice
	Two-way ANOVA for WT and p11 KO mice
	Group effect	Two-way ANOVA	F_(1,80) = 0.1875	p = 0.6661
	Time effect	Two-way ANOVA	F_(7,80) = 12.2601	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(7,80) = 0.7459	p = 0.6339

H: DA levels in the PFC with exposure to female mice (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₃₅₎ = 5.76	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₃₅₎ = 7.14	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₃₅₎ = 3.72	p = 0.0007
	Basal vs 80 min	Mixed linear models	t₍₃₅₎ = 1.28	p = 0.2092
	Basal vs 100 min	Mixed linear models	t₍₃₅₎ = -0.05	p = 0.9614
H: DA levels in the PFC with exposure to female mice (p11 KO mice)
	Basal vs 20 min	Mixed linear models	t₍₃₅₎ = 3.34	p = 0.002
	Basal vs 40 min	Mixed linear models	t₍₃₅₎ = 6.59	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₃₅₎ = 3.54	p = 0.0011
	Basal vs 80 min	Mixed linear models	t₍₃₅₎ = 1.89	p = 0.0675
	Basal vs 100 min	Mixed linear models	t₍₃₅₎ = 2.16	p = 0.0373

Figure 2
A: DA levels in the NAc with cocaine and/or nicotine infusion in p11 KO mice
	Two-way ANOVA for cocaine and cocaine + nicotine infusion
	Group effect	Two-way ANOVA	F_(1,120) = 45.9468	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,120) = 9.0389	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,120) = 3.0465	p = 0.0026
	Two-way ANOVA for nicotine and cocaine + nicotine infusion
	Group effect	Two-way ANOVA	F_(1,110) = 83.0855	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,110) = 4.9164	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,110) = 5.2703	p < 0.0001
A: DA levels in the NAc with cocaine and nicotine infusion in p11 KO mice
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 1.32	p = 0.1932
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 4.37	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 6.19	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 6.05	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 5.03	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 5.53	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 5.46	p < 0.0001
A: DA levels in the NAc with nicotine infusion in p11 KO mice
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = -0.46	p = 0.6509
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 0.09	p = 0.9282
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 0.21	p = 0.8359
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = -0.03	p = 0.9802
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = -0.07	p = 0.9407
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 0.08	p = 0.9383
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = -1.49	p = 0.1437
B: DA levels in the NAc with cocaine and/or oxotremorine infusion in p11 KO mice
	Two-way ANOVA for cocaine and cocaine + oxotremorine infusion
	Group effect	Two-way ANOVA	F_(1,120) = 89.7480	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,120) = 13.8003	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,120) = 5.6135	p < 0.0001
	Two-way ANOVA for oxotremorine and cocaine + oxotremorine infusion
	Group effect	Two-way ANOVA	F_(1,110) = 72.5608	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,110) = 8.88318	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,110) = 5.3849	p < 0.0001
B: DA levels in the NAc with cocaine and oxotremorine infusion in p11 KO mice
	Basal vs 20 min	Mixed linear models	t₍₅₄₎ = 1.72	p = 0.0907
	Basal vs 40 min	Mixed linear models	t₍₅₄₎ = 6.21	p < 0.0001

	Basal vs 60 min	Mixed linear models	t₍₅₄₎ = 7.6	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₅₄₎ = 8.13	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₅₄₎ = 9.28	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₅₄₎ = 7.82	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₅₄₎ = 7.47	p < 0.0001
B: DA levels in the NAc with oxotremorine infusion in p11 KO mice
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = 1.25	p = 0.2171
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 1.42	p = 0.1612
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 2.26	p = 0.029
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 1.37	p = 0.1774
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 0.75	p = 0.4567
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 1.88	p = 0.0661
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 0.66	p = 0.5144
Figure 3
A: DA levels in the NAc with cocaine infusion
	Two-way ANOVA for WT and ChAT-p11 cKO mice
	Group effect	Two-way ANOVA	F_(1,140) = 108.3406	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,140) = 21.5972	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,140) = 6.8674	p < 0.0001
A: DA levels in the NAc with cocaine infusion into the NAc (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 2.24	p = 0.0288
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 6.09	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 8.34	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 9.09	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 9	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 9.97	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 9.71	p < 0.0001
A: DA levels in the NAc with cocaine infusion into the NAc (ChAT-p11 cKO mice)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 0.28	p = 0.7771
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 4.04	p = 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 4.2	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 4.44	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 4.82	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 4.84	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 5.38	p < 0.0001
B: DA levels in the NAc with exposure to palatable food
	Two-way ANOVA for WT and ChAT-p11 cKO mice
	Group effect	Two-way ANOVA	F_(1,140) = 29.2503	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,140) = 2.5700	p = 0.0091
	Group-time interaction	Two-way ANOVA	F_(9,140) = 3.0056	p = 0.0026
B: DA levels in the NAc with exposure to palatable food (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 5.14	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 6.24	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 2.91	p = 0.005
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 1.95	p = 0.0554
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 1.96	p = 0.0541
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 1.08	p = 0.284
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 1.77	p = 0.0816
B: DA levels in the NAc with exposure to palatable food (ChAT-p11 cKO mice)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = -0.43	p = 0.6675
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = -0.31	p = 0.7584
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = -0.45	p = 0.6577
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = -0.72	p = 0.4734

	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 0.13	p = 0.9005
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 0.2	p = 0.8398
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = -0.31	p = 0.7576
C: DA levels in the NAc with exposure to female mice
	Two-way ANOVA for WT and ChAT-p11 cKO mice
	Group effect	Two-way ANOVA	F_(1,112) = 31.1748	p < 0.0001
	Time effect	Two-way ANOVA	F_(7,112) = 6.5263	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(7,112) = 4.9259	p < 0.0001
C: DA levels in the NAc with exposure to female mice (WT mice)
	Basal vs 20 min	Mixed linear models	t₍₄₉₎ = 5	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₄₉₎ = 6.39	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₄₉₎ = 3.29	p = 0.0018
	Basal vs 80 min	Mixed linear models	t₍₄₉₎ = 1.8	p = 0.0785
	Basal vs 100 min	Mixed linear models	t₍₄₉₎ = 1.79	p = 0.0802
C: DA levels in the NAc with exposure to female mice (ChAT-p11 cKO mice)
	Basal vs 20 min	Mixed linear models	t₍₄₉₎ = 0.37	p = 0.7103
	Basal vs 40 min	Mixed linear models	t₍₄₉₎ = 1.12	p = 0.2681
	Basal vs 60 min	Mixed linear models	t₍₄₉₎ = 2.51	p = 0.0153
	Basal vs 80 min	Mixed linear models	t₍₄₉₎ = 1.24	p = 0.2202
	Basal vs 100 min	Mixed linear models	t₍₄₉₎ = 0.02	p = 0.9862
Figure 4
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP
	Two-way ANOVA for AAV-p11 and AAV-YFP
	Group effect	Two-way ANOVA	F_(1,140) = 39.4565	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,140) = 8.6938	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,140) = 2.6737	p < 0.0001
C: DA levels in the NAc with cocaine infusion into the NAc (p11 cKO + AAV-YFP)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 0.17	p = 0.8632
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 4.83	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 5.37	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 5.71	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 6.91	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 5.5	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 4.29	p < 0.0001
C: DA levels in the NAc with cocaine infusion into the NAc (p11 cKO + AAV-p11)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 0.91	p = 0.3647
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 4.15	p = 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 5.59	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 6.41	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 6.22	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 5.92	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 4.92	p < 0.0001
D: DA levels in the NAc with exposure to palatable food in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP
	Two-way ANOVA for AAV-p11 and AAV-YFP
	Group effect	Two-way ANOVA	F_(1,140) = 57.9163	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,140) = 3.8107	p = 0.0003
	Group-time interaction	Two-way ANOVA	F_(9,140) = 3.4534	p = 0.0007
D: DA levels in the NAc with exposure to palatable food (p11 cKO + AAV-YFP)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = -0.54	p = 0.5909
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 0.75	p = 0.4536
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = -2.08	p = 0.0412

	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = -2.62	p = 0.0111
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = -2.21	p = 0.0308
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = -1.85	p = 0.0695
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = -3.65	p = 0.0005
D: DA levels in the NAc with exposure to palatable food (p11 cKO + AAV-p11)
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 4.45	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 6.65	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 3.76	p = 0.0004
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 3.66	p = 0.0005
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 2.07	p = 0.0424
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 2.2	p = 0.0315
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 0.98	p = 0.3308
E: DA levels in the NAc with exposure to female mice in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP
	Two-way ANOVA for AAV-p11 and AAV-YFP
	Group effect	Two-way ANOVA	F_(1,112) = 25.2729	p < 0.0001
	Time effect	Two-way ANOVA	F_(7,112) = 5.4068	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(7,112) = 2.5674	p = 0.0172
E: DA levels in the NAc with exposure to female mice (p11 cKO + AAV-YFP)
	Basal vs 20 min	Mixed linear models	t₍₄₉₎ = 0.62	p = 0.5353
	Basal vs 40 min	Mixed linear models	t₍₄₉₎ = 1.92	p = 0.0608
	Basal vs 60 min	Mixed linear models	t₍₄₉₎ = 0.25	p = 0.8038
	Basal vs 80 min	Mixed linear models	t₍₄₉₎ = -1.14	p = 0.2615
	Basal vs 100 min	Mixed linear models	t₍₄₉₎ = -0.64	p = 0.5276
E: DA levels in the NAc with exposure to female mice (p11 cKO + AAV-p11)
	Basal vs 20 min	Mixed linear models	t₍₄₉₎ = 5.9	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₄₉₎ = 5.69	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₄₉₎ = 3.21	p = 0.0023
	Basal vs 80 min	Mixed linear models	t₍₄₉₎ = 2.05	p = 0.0458
	Basal vs 100 min	Mixed linear models	t₍₄₉₎ = 2.38	p = 0.0212
Figure 5
A: ACh levels in the NAc with cocaine infusion
	Two-way ANOVA for WT and ChAT-p11 cKO mice
	Group effect	Two-way ANOVA	F_{(1,324 )} = 35.3923	p < 0.0001
	Time effect	Two-way ANOVA	F_{(17,324 )} = 0.9289	p = 0.5400
	Group-time interaction	Two-way ANOVA	F_(17,324) = 1.7341	p = 0.0358
A: ACh levels in the NAc with cocaine infusion into the NAc (WT mice)
	Basal vs 10 min	Mixed linear models	t₍₁₈₇₎ = -0.14	p = 0.8895
	Basal vs 20 min	Mixed linear models	t₍₁₈₇₎ = 4.07	p < 0.0001
	Basal vs 30 min	Mixed linear models	t₍₁₈₇₎ = 2.86	p = 0.0047
	Basal vs 40 min	Mixed linear models	t₍₁₈₇₎ = 2.65	p = 0.0087
	Basal vs 50 min	Mixed linear models	t₍₁₈₇₎ = 3.73	p = 0.0003
	Basal vs 60 min	Mixed linear models	t₍₁₈₇₎ = 3.55	p = 0.0005
	Basal vs 70 min	Mixed linear models	t₍₁₈₇₎ = 4.11	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₁₈₇₎ = 3.23	p = 0.0015
	Basal vs 90 min	Mixed linear models	t₍₁₈₇₎ = 2.53	p = 0.0123
	Basal vs 100 min	Mixed linear models	t₍₁₈₇₎ = 2.92	p = 0.0039
	Basal vs 110 min	Mixed linear models	t₍₁₈₇₎ = 2.76	p = 0.0063
	Basal vs 120 min	Mixed linear models	t₍₁₈₇₎ = 3.29	p = 0.0012
A: ACh levels in the NAc with cocaine infusion into the NAc (ChAT-p11 cKO mice)
	Basal vs 10 min	Mixed linear models	t₍₁₁₉₎ = -0.55	p = 0.5808
	Basal vs 20 min	Mixed linear models	t₍₁₁₉₎ = -1.2	p = 0.2334
	Basal vs 30 min	Mixed linear models	t₍₁₁₉₎ = -1.98	p = 0.0502

	Basal vs 40 min	Mixed linear models	t₍₁₁₉₎ = -2.38	p = 0.0191
	Basal vs 50 min	Mixed linear models	t₍₁₁₉₎ = -0.46	p = 0.6487
	Basal vs 60 min	Mixed linear models	t₍₁₁₉₎ = 0.21	p = 0.835
	Basal vs 70 min	Mixed linear models	t₍₁₁₉₎ = -0.44	p = 0.6587
	Basal vs 80 min	Mixed linear models	t₍₁₁₉₎ = -1.03	p = 0.3053
	Basal vs 90 min	Mixed linear models	t₍₁₁₉₎ = -1.36	p = 0.1767
	Basal vs 100 min	Mixed linear models	t₍₁₁₉₎ = -0.44	p = 0.6627
	Basal vs 110 min	Mixed linear models	t₍₁₁₉₎ = 0.07	p = 0.9468
	Basal vs 120 min	Mixed linear models	t₍₁₁₉₎ = 0.39	p = 0.6987
Figure 6
B: DA levels in the NAc with CNO infusion in ChAT-p11 cKO mice injected with AAV-rM3D or AAV-mCherry		One-way ANOVA	F_(3,20) = 7.643	p = 0.0014
	AAV-mCherry/CNO 10 µM vs AAV-rM3D/CNO 10 µM	Newman–Keuls post hoc test		p < 0.01
	AAV-rM3D/CNO 3 µM vs AAV-rM3D/CNO 10 µM	Newman–Keuls post hoc test		p < 0.01
C: DA levels in the NAc with cocaine or CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D or AAV-mCherry
	Two-way ANOVA for AAV-rM3D/CNO + cocaine or AAV-mCherry/CNO + cocaine
	Group effect	Two-way ANOVA	F_(1,100) = 94.7020	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,100) = 23.4516	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,100) = 5.7876	p < 0.0001
	Two-way ANOVA for AAV-rM3D/CNO + cocaine or AAV-rM3D/cocaine
	Group effect	Two-way ANOVA	F_(1,100) = 106.4829	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,100) = 15.2109	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,100) = 6.4710	p < 0.0001
C: DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = 2.53	p = 0.015
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 7.21	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 6.78	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 7.95	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 8.68	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 7.87	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 10.11	p < 0.0001
C: DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = 0.64	p = 0.5243
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 3.98	p = 0.0002
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 6.48	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 5.56	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 6.59	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 6.36	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 6.33	p < 0.0001
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = -0.45	p = 0.6554
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 2.43	p = 0.0192
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 2.96	p = 0.0049
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 1.85	p = 0.0709
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 1.59	p = 0.1184
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 2.56	p = 0.014
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 2.99	p = 0.0045
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = 0.81	p = 0.4199
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 0.81	p = 0.0002

	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 0.81	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 0.81	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 0.81	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 0.81	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 0.81	p < 0.0001
Figure 7
DA levels in the NAc with cocaine or CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D or AAV-mCherry
	Two-way ANOVA for AAV-hM4D/CNO + cocaine or AAV-mCherry/CNO + cocaine
	Group effect	Two-way ANOVA	F_(1,150) = 12.3097	p = 0.0006
	Time effect	Two-way ANOVA	F_(9,150) = 17.6639	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,150) = 1.2133	p = 0.2908
	Two-way ANOVA for AAV-hM4D/CNO + cocaine or AAV-hM4D/cocaine
	Group effect	Two-way ANOVA	F_(1,220) = 32.4559	p < 0.0001
	Time effect	Two-way ANOVA	F_(9,220) = 14.3297	p < 0.0001
	Group-time interaction	Two-way ANOVA	F_(9,220) = 1.7342	p = 0.0826
DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D
	Basal vs 20 min	Mixed linear models	t₍₉₀₎ = 2.68	p = 0.0087
	Basal vs 40 min	Mixed linear models	t₍₉₀₎ = 4.63	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₉₀₎ = 7.40	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₉₀₎ = 7.17	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₉₀₎ = 6.77	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₉₀₎ = 6.99	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₉₀₎ = 7.22	p < 0.0001
DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry
	Basal vs 20 min	Mixed linear models	t₍₄₅₎ = -0.11	p = 0.9112
	Basal vs 40 min	Mixed linear models	t₍₄₅₎ = 3.79	p = 0.0005
	Basal vs 60 min	Mixed linear models	t₍₄₅₎ = 6.43	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₄₅₎ = 5.87	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₄₅₎ = 5.20	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₄₅₎ = 5.13	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₄₅₎ = 5.28	p < 0.0001
DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D
	Basal vs 20 min	Mixed linear models	t₍₁₀₈₎ = 4.40	p < 0.0001
	Basal vs 40 min	Mixed linear models	t₍₁₀₈₎ = 7.77	p < 0.0001
	Basal vs 60 min	Mixed linear models	t₍₁₀₈₎ = 8.47	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₁₀₈₎ = 8.23	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₁₀₈₎ = 8.63	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₁₀₈₎ = 8.33	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₁₀₈₎ = 7.47	p < 0.0001
DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry
	Basal vs 20 min	Mixed linear models	t₍₆₃₎ = 0.81	p = 0.4199
	Basal vs 40 min	Mixed linear models	t₍₆₃₎ = 4.02	p = 0.0002
	Basal vs 60 min	Mixed linear models	t₍₆₃₎ = 5.64	p < 0.0001
	Basal vs 80 min	Mixed linear models	t₍₆₃₎ = 6.52	p < 0.0001
	Basal vs 100 min	Mixed linear models	t₍₆₃₎ = 5.89	p < 0.0001
	Basal vs 120 min	Mixed linear models	t₍₆₃₎ = 5.89	p < 0.0001
	Basal vs 140 min	Mixed linear models	t₍₆₃₎ = 6.00	p < 0.0001
Table 1
Basal levels of dopamine, dopamine metabolites, and ACh
	NAc DA, WT vs p11 KO	t test	t₍₃₉₎ = 0.1283	p = 0.8986
	NAc DOPAC, WT vs p11 KO	t test	t₍₃₅₎ = 0.04948	p = 0.9608
	NAc HVA, WT vs p11 KO	t test	t₍₂₂₎ = 0.2247	p = 0.8243

	PFC DA, WT vs p11 KO	t test (Welch's-correction)	t₍₁₃₎ = 1.183	p = 0.2672
	PFC DOPAC, WT vs p11 KO	t test	t₍₁₂₎ = 0.6739	p = 0.5132
	PFC HVA, WT vs p11 KO	t test	t₍₅₎ = 0.1866	p = 0.8593
	NAc DA, WT vs ChAT-p11 cKO	t test	t₍₁₄₎ = 1.139	p = 0.2739
	NAc DOPAC, WT vs ChAT-p11 cKO	t test	t₍₁₄₎ = 0.04194	p = 0.9671
	NAc ACh, WT vs ChAT-p11 cKO	t test	t₍₁₈₎ = 0.9686	p = 0.3456
	NAc DA, ChAT-p11 cKO + AAV-YFP vs ChAT-p11 cKO + AAV-p11	t test	t₍₁₄₎ = 0.5145	p = 0.6149
	NAc DA, ChAT-p11 cKO + AAV-mCherry vs ChAT-p11 cKO + AAV-rM3D(Gs)	t test	t₍₁₀₎ = 0.1095	p = 0.9150
	NAc DA, ChAT-p11 cKO + AAV-mCherry vs ChAT-p11 cKO + AAV-hM4D (Gi)	t test	t₍₁₉₎ = 1.011	p = 0.3246