Figure 1 | | | |
C: DA levels in the NAc with cocaine infusion into the NAc | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,120) = 49.4312 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,120) = 9.4748 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 4.1100 | p = 0.0001 |
C: DA levels in the NAc with cocaine infusion into the NAc (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 1.2 | p = 0.2351 |
| Basal vs 40 min | Mixed linear models | t(54) = 5.5 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(54) = 6.54 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 7.04 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 6.53 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 5.23 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 4.39 | p < 0.0001 |
C: DA levels in the NAc with cocaine infusion into the NAc (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = -0.69 | p = 0.4942 |
| Basal vs 40 min | Mixed linear models | t(54) = 0.3 | p = 0.7639 |
| Basal vs 60 min | Mixed linear models | t(54) = 1.54 | p = 0.1286 |
| Basal vs 80 min | Mixed linear models | t(54) = 1.79 | p = 0.0783 |
| Basal vs 100 min | Mixed linear models | t(54) = 2.19 | p = 0.0326 |
| Basal vs 120 min | Mixed linear models | t(54) = 3.78 | p = 0.0004 |
| Basal vs 140 min | Mixed linear models | t(54) = 2.86 | p = 0.0059 |
D: DA levels in the NAc with exposure to palatable food | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,120) = 37.1184 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,120) = 3.4984 | p = 0.0007 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 2.3706 | p = 0.0167 |
D: DA levels in the NAc with exposure to palatable food (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 2.08 | p = 0.0421 |
| Basal vs 40 min | Mixed linear models | t(54) = 4.42 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(54) = 5.64 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 4.7 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 3.15 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 2.71 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 2.23 | p < 0.0001 |
D: DA levels in the NAc with exposure to palatable food (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 1.15 | p = 0.2544 |
| Basal vs 40 min | Mixed linear models | t(54) = 2.03 | p = 0.0475 |
| Basal vs 60 min | Mixed linear models | t(54) = 1.72 | p = 0.0911 |
| Basal vs 80 min | Mixed linear models | t(54) = -0.07 | p = 0.9414 |
| Basal vs 100 min | Mixed linear models | t(54) = -1.7 | p = 0.095 |
| Basal vs 120 min | Mixed linear models | t(54) = -0.72 | p = 0.4769 |
| Basal vs 140 min | Mixed linear models | t(54) = -0.74 | p = 0.464 |
E: DA levels in the NAc with exposure to female mice | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,80) = 39.2674 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(7,80) = 7.0594 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(7,80) = 3.7936 | p = 0.0013 |
E: DA levels in the NAc with exposure to female mice (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(35) = 5.69 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(35) = 8.36 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(35) = 5.29 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(35) = 3.39 | p = 0.0017 |
| Basal vs 100 min | Mixed linear models | t(35) = 3.06 | p = 0.0042 |
|
E: DA levels in the NAc with exposure to female mice (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(35) = 0.33 | p = 0.7429 |
| Basal vs 40 min | Mixed linear models | t(35) = 2.21 | p = 0.0335 |
| Basal vs 60 min | Mixed linear models | t(35) = 0.46 | p = 0.6483 |
| Basal vs 80 min | Mixed linear models | t(35) = 0.17 | p = 0.865 |
| Basal vs 100 min | Mixed linear models | t(35) = -1.9 | p = 0.5526 |
F: DA levels in the PFC with cocaine infusion | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,120) = 0.00970 | p = 0.7560 |
| Time effect | Two-way ANOVA | F(9,120) = 8.8283 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 0.00895 | p = 0.9997 |
F: DA levels in the PFC with cocaine infusion into the PFC (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 0.9 | p = 0.3719 |
| Basal vs 40 min | Mixed linear models | t(54) = 3.04 | p = 0.0037 |
| Basal vs 60 min | Mixed linear models | t(54) = 4.29 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 5.3 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 5.98 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 4.86 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 4.23 | p < 0.0001 |
F: DA levels in the PFC with cocaine infusion into the PFC (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 1.06 | p = 0.2933 |
| Basal vs 40 min | Mixed linear models | t(54) = 3.26 | p = 0.0019 |
| Basal vs 60 min | Mixed linear models | t(54) = 4.61 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 5.4 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 4.94 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 5.18 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 5.09 | p < 0.0001 |
G: DA levels in the PFC with exposure to palatable food | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,120) = 0.0733 | p = 0.7870 |
| Time effect | Two-way ANOVA | F(9,120) = 11.4806 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 0.1100 | p = 0.9994 |
G: DA levels in the PFC with exposure to palatable food (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 4.23 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(54) = 5.92 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(54) = 4.67 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 4.22 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 3.99 | p = 0.0002 |
| Basal vs 120 min | Mixed linear models | t(54) = 2.54 | p = 0.0139 |
| Basal vs 140 min | Mixed linear models | t(54) = 1.9 | p = 0.0631 |
G: DA levels in the PFC with exposure to palatable food (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 4.55 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(54) = 5.86 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(54) = 5.41 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 4.84 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 4.42 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 3.37 | p = 0.0014 |
| Basal vs 140 min | Mixed linear models | t(54) = 3.14 | p = 0.0028 |
H: DA levels in the PFC with exposure to female mice | | | |
| Two-way ANOVA for WT and p11 KO mice | | | |
| Group effect | Two-way ANOVA | F(1,80) = 0.1875 | p = 0.6661 |
| Time effect | Two-way ANOVA | F(7,80) = 12.2601 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(7,80) = 0.7459 | p = 0.6339 |
|
H: DA levels in the PFC with exposure to female mice (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(35) = 5.76 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(35) = 7.14 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(35) = 3.72 | p = 0.0007 |
| Basal vs 80 min | Mixed linear models | t(35) = 1.28 | p = 0.2092 |
| Basal vs 100 min | Mixed linear models | t(35) = -0.05 | p = 0.9614 |
H: DA levels in the PFC with exposure to female mice (p11 KO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(35) = 3.34 | p = 0.002 |
| Basal vs 40 min | Mixed linear models | t(35) = 6.59 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(35) = 3.54 | p = 0.0011 |
| Basal vs 80 min | Mixed linear models | t(35) = 1.89 | p = 0.0675 |
| Basal vs 100 min | Mixed linear models | t(35) = 2.16 | p = 0.0373 |
| | | | |
Figure 2 |
A: DA levels in the NAc with cocaine and/or nicotine infusion in p11 KO mice | | | |
| Two-way ANOVA for cocaine and cocaine + nicotine infusion | | | |
| Group effect | Two-way ANOVA | F(1,120) = 45.9468 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,120) = 9.0389 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 3.0465 | p = 0.0026 |
| Two-way ANOVA for nicotine and cocaine + nicotine infusion | | | |
| Group effect | Two-way ANOVA | F(1,110) = 83.0855 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,110) = 4.9164 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,110) = 5.2703 | p < 0.0001 |
A: DA levels in the NAc with cocaine and nicotine infusion in p11 KO mice | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 1.32 | p = 0.1932 |
| Basal vs 40 min | Mixed linear models | t(54) = 4.37 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(54) = 6.19 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 6.05 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 5.03 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 5.53 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 5.46 | p < 0.0001 |
A: DA levels in the NAc with nicotine infusion in p11 KO mice | | | |
| Basal vs 20 min | Mixed linear models | t(45) = -0.46 | p = 0.6509 |
| Basal vs 40 min | Mixed linear models | t(45) = 0.09 | p = 0.9282 |
| Basal vs 60 min | Mixed linear models | t(45) = 0.21 | p = 0.8359 |
| Basal vs 80 min | Mixed linear models | t(45) = -0.03 | p = 0.9802 |
| Basal vs 100 min | Mixed linear models | t(45) = -0.07 | p = 0.9407 |
| Basal vs 120 min | Mixed linear models | t(45) = 0.08 | p = 0.9383 |
| Basal vs 140 min | Mixed linear models | t(45) = -1.49 | p = 0.1437 |
B: DA levels in the NAc with cocaine and/or oxotremorine infusion in p11 KO mice | | | |
| Two-way ANOVA for cocaine and cocaine + oxotremorine infusion | | | |
| Group effect | Two-way ANOVA | F(1,120) = 89.7480 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,120) = 13.8003 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,120) = 5.6135 | p < 0.0001 |
| Two-way ANOVA for oxotremorine and cocaine + oxotremorine infusion | | | |
| Group effect | Two-way ANOVA | F(1,110) = 72.5608 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,110) = 8.88318 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,110) = 5.3849 | p < 0.0001 |
B: DA levels in the NAc with cocaine and oxotremorine infusion in p11 KO mice | | | |
| Basal vs 20 min | Mixed linear models | t(54) = 1.72 | p = 0.0907 |
| Basal vs 40 min | Mixed linear models | t(54) = 6.21 | p < 0.0001 |
|
| Basal vs 60 min | Mixed linear models | t(54) = 7.6 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(54) = 8.13 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(54) = 9.28 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(54) = 7.82 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(54) = 7.47 | p < 0.0001 |
B: DA levels in the NAc with oxotremorine infusion in p11 KO mice | | | |
| Basal vs 20 min | Mixed linear models | t(45) = 1.25 | p = 0.2171 |
| Basal vs 40 min | Mixed linear models | t(45) = 1.42 | p = 0.1612 |
| Basal vs 60 min | Mixed linear models | t(45) = 2.26 | p = 0.029 |
| Basal vs 80 min | Mixed linear models | t(45) = 1.37 | p = 0.1774 |
| Basal vs 100 min | Mixed linear models | t(45) = 0.75 | p = 0.4567 |
| Basal vs 120 min | Mixed linear models | t(45) = 1.88 | p = 0.0661 |
| Basal vs 140 min | Mixed linear models | t(45) = 0.66 | p = 0.5144 |
Figure 3 | | | | |
A: DA levels in the NAc with cocaine infusion | | | |
| Two-way ANOVA for WT and ChAT-p11 cKO mice | | | |
| Group effect | Two-way ANOVA | F(1,140) = 108.3406 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,140) = 21.5972 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,140) = 6.8674 | p < 0.0001 |
A: DA levels in the NAc with cocaine infusion into the NAc (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 2.24 | p = 0.0288 |
| Basal vs 40 min | Mixed linear models | t(63) = 6.09 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 8.34 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(63) = 9.09 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(63) = 9 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(63) = 9.97 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(63) = 9.71 | p < 0.0001 |
A: DA levels in the NAc with cocaine infusion into the NAc (ChAT-p11 cKO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 0.28 | p = 0.7771 |
| Basal vs 40 min | Mixed linear models | t(63) = 4.04 | p = 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 4.2 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(63) = 4.44 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(63) = 4.82 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(63) = 4.84 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(63) = 5.38 | p < 0.0001 |
B: DA levels in the NAc with exposure to palatable food | | | |
| Two-way ANOVA for WT and ChAT-p11 cKO mice | | | |
| Group effect | Two-way ANOVA | F(1,140) = 29.2503 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,140) = 2.5700 | p = 0.0091 |
| Group-time interaction | Two-way ANOVA | F(9,140) = 3.0056 | p = 0.0026 |
B: DA levels in the NAc with exposure to palatable food (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 5.14 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(63) = 6.24 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 2.91 | p = 0.005 |
| Basal vs 80 min | Mixed linear models | t(63) = 1.95 | p = 0.0554 |
| Basal vs 100 min | Mixed linear models | t(63) = 1.96 | p = 0.0541 |
| Basal vs 120 min | Mixed linear models | t(63) = 1.08 | p = 0.284 |
| Basal vs 140 min | Mixed linear models | t(63) = 1.77 | p = 0.0816 |
B: DA levels in the NAc with exposure to palatable food (ChAT-p11 cKO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = -0.43 | p = 0.6675 |
| Basal vs 40 min | Mixed linear models | t(63) = -0.31 | p = 0.7584 |
| Basal vs 60 min | Mixed linear models | t(63) = -0.45 | p = 0.6577 |
| Basal vs 80 min | Mixed linear models | t(63) = -0.72 | p = 0.4734 |
|
| Basal vs 100 min | Mixed linear models | t(63) = 0.13 | p = 0.9005 |
| Basal vs 120 min | Mixed linear models | t(63) = 0.2 | p = 0.8398 |
| Basal vs 140 min | Mixed linear models | t(63) = -0.31 | p = 0.7576 |
C: DA levels in the NAc with exposure to female mice | | | |
| Two-way ANOVA for WT and ChAT-p11 cKO mice | | | |
| Group effect | Two-way ANOVA | F(1,112) = 31.1748 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(7,112) = 6.5263 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(7,112) = 4.9259 | p < 0.0001 |
C: DA levels in the NAc with exposure to female mice (WT mice) | | | |
| Basal vs 20 min | Mixed linear models | t(49) = 5 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(49) = 6.39 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(49) = 3.29 | p = 0.0018 |
| Basal vs 80 min | Mixed linear models | t(49) = 1.8 | p = 0.0785 |
| Basal vs 100 min | Mixed linear models | t(49) = 1.79 | p = 0.0802 |
C: DA levels in the NAc with exposure to female mice (ChAT-p11 cKO mice) | | | |
| Basal vs 20 min | Mixed linear models | t(49) = 0.37 | p = 0.7103 |
| Basal vs 40 min | Mixed linear models | t(49) = 1.12 | p = 0.2681 |
| Basal vs 60 min | Mixed linear models | t(49) = 2.51 | p = 0.0153 |
| Basal vs 80 min | Mixed linear models | t(49) = 1.24 | p = 0.2202 |
| Basal vs 100 min | Mixed linear models | t(49) = 0.02 | p = 0.9862 |
Figure 4 | | | | |
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP | | | |
| Two-way ANOVA for AAV-p11 and AAV-YFP | | | |
| Group effect | Two-way ANOVA | F(1,140) = 39.4565 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,140) = 8.6938 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,140) = 2.6737 | p < 0.0001 |
C: DA levels in the NAc with cocaine infusion into the NAc (p11 cKO + AAV-YFP) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 0.17 | p = 0.8632 |
| Basal vs 40 min | Mixed linear models | t(63) = 4.83 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 5.37 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(63) = 5.71 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(63) = 6.91 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(63) = 5.5 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(63) = 4.29 | p < 0.0001 |
C: DA levels in the NAc with cocaine infusion into the NAc (p11 cKO + AAV-p11) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 0.91 | p = 0.3647 |
| Basal vs 40 min | Mixed linear models | t(63) = 4.15 | p = 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 5.59 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(63) = 6.41 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(63) = 6.22 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(63) = 5.92 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(63) = 4.92 | p < 0.0001 |
D: DA levels in the NAc with exposure to palatable food in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP | | | |
| Two-way ANOVA for AAV-p11 and AAV-YFP | | | |
| Group effect | Two-way ANOVA | F(1,140) = 57.9163 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,140) = 3.8107 | p = 0.0003 |
| Group-time interaction | Two-way ANOVA | F(9,140) = 3.4534 | p = 0.0007 |
D: DA levels in the NAc with exposure to palatable food (p11 cKO + AAV-YFP) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = -0.54 | p = 0.5909 |
| Basal vs 40 min | Mixed linear models | t(63) = 0.75 | p = 0.4536 |
| Basal vs 60 min | Mixed linear models | t(63) = -2.08 | p = 0.0412 |
|
| Basal vs 80 min | Mixed linear models | t(63) = -2.62 | p = 0.0111 |
| Basal vs 100 min | Mixed linear models | t(63) = -2.21 | p = 0.0308 |
| Basal vs 120 min | Mixed linear models | t(63) = -1.85 | p = 0.0695 |
| Basal vs 140 min | Mixed linear models | t(63) = -3.65 | p = 0.0005 |
D: DA levels in the NAc with exposure to palatable food (p11 cKO + AAV-p11) | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 4.45 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(63) = 6.65 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(63) = 3.76 | p = 0.0004 |
| Basal vs 80 min | Mixed linear models | t(63) = 3.66 | p = 0.0005 |
| Basal vs 100 min | Mixed linear models | t(63) = 2.07 | p = 0.0424 |
| Basal vs 120 min | Mixed linear models | t(63) = 2.2 | p = 0.0315 |
| Basal vs 140 min | Mixed linear models | t(63) = 0.98 | p = 0.3308 |
E: DA levels in the NAc with exposure to female mice in ChAT-p11 cKO mice injected with AAV-p11 or AAV-YFP | | | |
| Two-way ANOVA for AAV-p11 and AAV-YFP | | | |
| Group effect | Two-way ANOVA | F(1,112) = 25.2729 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(7,112) = 5.4068 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(7,112) = 2.5674 | p = 0.0172 |
E: DA levels in the NAc with exposure to female mice (p11 cKO + AAV-YFP) | | | |
| Basal vs 20 min | Mixed linear models | t(49) = 0.62 | p = 0.5353 |
| Basal vs 40 min | Mixed linear models | t(49) = 1.92 | p = 0.0608 |
| Basal vs 60 min | Mixed linear models | t(49) = 0.25 | p = 0.8038 |
| Basal vs 80 min | Mixed linear models | t(49) = -1.14 | p = 0.2615 |
| Basal vs 100 min | Mixed linear models | t(49) = -0.64 | p = 0.5276 |
E: DA levels in the NAc with exposure to female mice (p11 cKO + AAV-p11) | | | |
| Basal vs 20 min | Mixed linear models | t(49) = 5.9 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(49) = 5.69 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(49) = 3.21 | p = 0.0023 |
| Basal vs 80 min | Mixed linear models | t(49) = 2.05 | p = 0.0458 |
| Basal vs 100 min | Mixed linear models | t(49) = 2.38 | p = 0.0212 |
Figure 5 | | | | |
A: ACh levels in the NAc with cocaine infusion | | | |
| Two-way ANOVA for WT and ChAT-p11 cKO mice | | | |
| Group effect | Two-way ANOVA | F(1,324 ) = 35.3923 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(17,324 ) = 0.9289 | p = 0.5400 |
| Group-time interaction | Two-way ANOVA | F(17,324) = 1.7341 | p = 0.0358 |
A: ACh levels in the NAc with cocaine infusion into the NAc (WT mice) | | | |
| Basal vs 10 min | Mixed linear models | t(187) = -0.14 | p = 0.8895 |
| Basal vs 20 min | Mixed linear models | t(187) = 4.07 | p < 0.0001 |
| Basal vs 30 min | Mixed linear models | t(187) = 2.86 | p = 0.0047 |
| Basal vs 40 min | Mixed linear models | t(187) = 2.65 | p = 0.0087 |
| Basal vs 50 min | Mixed linear models | t(187) = 3.73 | p = 0.0003 |
| Basal vs 60 min | Mixed linear models | t(187) = 3.55 | p = 0.0005 |
| Basal vs 70 min | Mixed linear models | t(187) = 4.11 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(187) = 3.23 | p = 0.0015 |
| Basal vs 90 min | Mixed linear models | t(187) = 2.53 | p = 0.0123 |
| Basal vs 100 min | Mixed linear models | t(187) = 2.92 | p = 0.0039 |
| Basal vs 110 min | Mixed linear models | t(187) = 2.76 | p = 0.0063 |
| Basal vs 120 min | Mixed linear models | t(187) = 3.29 | p = 0.0012 |
A: ACh levels in the NAc with cocaine infusion into the NAc (ChAT-p11 cKO mice) | | | |
| Basal vs 10 min | Mixed linear models | t(119) = -0.55 | p = 0.5808 |
| Basal vs 20 min | Mixed linear models | t(119) = -1.2 | p = 0.2334 |
| Basal vs 30 min | Mixed linear models | t(119) = -1.98 | p = 0.0502 |
|
| Basal vs 40 min | Mixed linear models | t(119) = -2.38 | p = 0.0191 |
| Basal vs 50 min | Mixed linear models | t(119) = -0.46 | p = 0.6487 |
| Basal vs 60 min | Mixed linear models | t(119) = 0.21 | p = 0.835 |
| Basal vs 70 min | Mixed linear models | t(119) = -0.44 | p = 0.6587 |
| Basal vs 80 min | Mixed linear models | t(119) = -1.03 | p = 0.3053 |
| Basal vs 90 min | Mixed linear models | t(119) = -1.36 | p = 0.1767 |
| Basal vs 100 min | Mixed linear models | t(119) = -0.44 | p = 0.6627 |
| Basal vs 110 min | Mixed linear models | t(119) = 0.07 | p = 0.9468 |
| Basal vs 120 min | Mixed linear models | t(119) = 0.39 | p = 0.6987 |
Figure 6 | | | | |
B: DA levels in the NAc with CNO infusion in ChAT-p11 cKO mice injected with AAV-rM3D or AAV-mCherry | One-way ANOVA | F(3,20) = 7.643 | p = 0.0014 |
| AAV-mCherry/CNO 10 µM vs AAV-rM3D/CNO 10 µM | Newman–Keuls post hoc test | | p < 0.01 |
| AAV-rM3D/CNO 3 µM vs AAV-rM3D/CNO 10 µM | Newman–Keuls post hoc test | | p < 0.01 |
C: DA levels in the NAc with cocaine or CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D or AAV-mCherry | | | |
| Two-way ANOVA for AAV-rM3D/CNO + cocaine or AAV-mCherry/CNO + cocaine | | | |
| Group effect | Two-way ANOVA | F(1,100) = 94.7020 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,100) = 23.4516 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,100) = 5.7876 | p < 0.0001 |
| Two-way ANOVA for AAV-rM3D/CNO + cocaine or AAV-rM3D/cocaine | | | |
| Group effect | Two-way ANOVA | F(1,100) = 106.4829 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,100) = 15.2109 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,100) = 6.4710 | p < 0.0001 |
C: DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D | | | |
| Basal vs 20 min | Mixed linear models | t(45) = 2.53 | p = 0.015 |
| Basal vs 40 min | Mixed linear models | t(45) = 7.21 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(45) = 6.78 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(45) = 7.95 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(45) = 8.68 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(45) = 7.87 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(45) = 10.11 | p < 0.0001 |
C: DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry | | | |
| Basal vs 20 min | Mixed linear models | t(45) = 0.64 | p = 0.5243 |
| Basal vs 40 min | Mixed linear models | t(45) = 3.98 | p = 0.0002 |
| Basal vs 60 min | Mixed linear models | t(45) = 6.48 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(45) = 5.56 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(45) = 6.59 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(45) = 6.36 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(45) = 6.33 | p < 0.0001 |
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-rM3D | | | |
| Basal vs 20 min | Mixed linear models | t(45) = -0.45 | p = 0.6554 |
| Basal vs 40 min | Mixed linear models | t(45) = 2.43 | p = 0.0192 |
| Basal vs 60 min | Mixed linear models | t(45) = 2.96 | p = 0.0049 |
| Basal vs 80 min | Mixed linear models | t(45) = 1.85 | p = 0.0709 |
| Basal vs 100 min | Mixed linear models | t(45) = 1.59 | p = 0.1184 |
| Basal vs 120 min | Mixed linear models | t(45) = 2.56 | p = 0.014 |
| Basal vs 140 min | Mixed linear models | t(45) = 2.99 | p = 0.0045 |
C: DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry | | | |
| Basal vs 20 min | Mixed linear models | t(45) = 0.81 | p = 0.4199 |
| Basal vs 40 min | Mixed linear models | t(45) = 0.81 | p = 0.0002 |
|
| Basal vs 60 min | Mixed linear models | t(45) = 0.81 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(45) = 0.81 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(45) = 0.81 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(45) = 0.81 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(45) = 0.81 | p < 0.0001 |
Figure 7 | | | | |
DA levels in the NAc with cocaine or CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D or AAV-mCherry | | | |
| Two-way ANOVA for AAV-hM4D/CNO + cocaine or AAV-mCherry/CNO + cocaine | | | |
| Group effect | Two-way ANOVA | F(1,150) = 12.3097 | p = 0.0006 |
| Time effect | Two-way ANOVA | F(9,150) = 17.6639 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,150) = 1.2133 | p = 0.2908 |
| Two-way ANOVA for AAV-hM4D/CNO + cocaine or AAV-hM4D/cocaine | | | |
| Group effect | Two-way ANOVA | F(1,220) = 32.4559 | p < 0.0001 |
| Time effect | Two-way ANOVA | F(9,220) = 14.3297 | p < 0.0001 |
| Group-time interaction | Two-way ANOVA | F(9,220) = 1.7342 | p = 0.0826 |
DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D | | | |
| Basal vs 20 min | Mixed linear models | t(90) = 2.68 | p = 0.0087 |
| Basal vs 40 min | Mixed linear models | t(90) = 4.63 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(90) = 7.40 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(90) = 7.17 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(90) = 6.77 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(90) = 6.99 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(90) = 7.22 | p < 0.0001 |
DA levels in the NAc with CNO + cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry | | | |
| Basal vs 20 min | Mixed linear models | t(45) = -0.11 | p = 0.9112 |
| Basal vs 40 min | Mixed linear models | t(45) = 3.79 | p = 0.0005 |
| Basal vs 60 min | Mixed linear models | t(45) = 6.43 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(45) = 5.87 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(45) = 5.20 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(45) = 5.13 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(45) = 5.28 | p < 0.0001 |
DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-hM4D | | | |
| Basal vs 20 min | Mixed linear models | t(108) = 4.40 | p < 0.0001 |
| Basal vs 40 min | Mixed linear models | t(108) = 7.77 | p < 0.0001 |
| Basal vs 60 min | Mixed linear models | t(108) = 8.47 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(108) = 8.23 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(108) = 8.63 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(108) = 8.33 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(108) = 7.47 | p < 0.0001 |
DA levels in the NAc with cocaine infusion in ChAT-p11 cKO mice injected with AAV-mCherry | | | |
| Basal vs 20 min | Mixed linear models | t(63) = 0.81 | p = 0.4199 |
| Basal vs 40 min | Mixed linear models | t(63) = 4.02 | p = 0.0002 |
| Basal vs 60 min | Mixed linear models | t(63) = 5.64 | p < 0.0001 |
| Basal vs 80 min | Mixed linear models | t(63) = 6.52 | p < 0.0001 |
| Basal vs 100 min | Mixed linear models | t(63) = 5.89 | p < 0.0001 |
| Basal vs 120 min | Mixed linear models | t(63) = 5.89 | p < 0.0001 |
| Basal vs 140 min | Mixed linear models | t(63) = 6.00 | p < 0.0001 |
Table 1 | | | | |
Basal levels of dopamine, dopamine metabolites, and ACh | | | |
| NAc DA, WT vs p11 KO | t test | t(39) = 0.1283 | p = 0.8986 |
| NAc DOPAC, WT vs p11 KO | t test | t(35) = 0.04948 | p = 0.9608 |
| NAc HVA, WT vs p11 KO | t test | t(22) = 0.2247 | p = 0.8243 |
|
| PFC DA, WT vs p11 KO | t test (Welch's-correction) | t(13) = 1.183 | p = 0.2672 |
| PFC DOPAC, WT vs p11 KO | t test | t(12) = 0.6739 | p = 0.5132 |
| PFC HVA, WT vs p11 KO | t test | t(5) = 0.1866 | p = 0.8593 |
| NAc DA, WT vs ChAT-p11 cKO | t test | t(14) = 1.139 | p = 0.2739 |
| NAc DOPAC, WT vs ChAT-p11 cKO | t test | t(14) = 0.04194 | p = 0.9671 |
| NAc ACh, WT vs ChAT-p11 cKO | t test | t(18) = 0.9686 | p = 0.3456 |
| NAc DA, ChAT-p11 cKO + AAV-YFP vs ChAT-p11 cKO + AAV-p11 | t test | t(14) = 0.5145 | p = 0.6149 |
| NAc DA, ChAT-p11 cKO + AAV-mCherry vs ChAT-p11 cKO + AAV-rM3D(Gs) | t test | t(10) = 0.1095 | p = 0.9150 |
| NAc DA, ChAT-p11 cKO + AAV-mCherry vs ChAT-p11 cKO + AAV-hM4D (Gi) | t test | t(19) = 1.011 | p = 0.3246 |