Detailed statistical analysis
| ANOVA | t test | ||||||
|---|---|---|---|---|---|---|---|
| Assay | Mouse line | Number | Figure | Genotype effect | Cell type/time/treatment | Interaction | |
| RT-qPCR | D1R-Gpr88 | N = 9 D1R-CTL; N = 9 D1R-Gpr88 | 1A (CPu) | t(16) = 3.01, p = 0.008 | |||
| N = 8 D1R-CTL; N = 7 D1R-Gpr88 | 1A (Nacc) | t(13) = 4.19, p = 0.001 | |||||
| N = 9 D1R-CTL; N = 7 D1R-Gpr88 | 1A (Hipp) | t(14) = 2.7, p = 0.017 | |||||
| N = 6 D1R-CTL; N = 7 D1R-Gpr88 | 1A (Amy) | t(11) = 0.53, p = 0. 6 | |||||
| [35S]-GTPgS binding | CMV-Gpr88; D1R-Gpr88 | N = 3 D1R-CTL, D1R-Gpr88, CMV-CTL, CMV-Gpr88 | 1B | F(3,66) = 185.2; p < 0.0001 | F(10,66) = 95.64; p < 0.0001 | F(30,66) = 23.19; p < 0.0001 | |
| In situ hybridization/cell counting | D1R-Gpr88 | N = 3 D1R-CTL, N = 3 D1R-Gpr88 | 2B (CPu) | F(1,134) = 0.4164; p = 0.5198 | F(1,134) = 957.2; p < 0.0001 | F(1,134) = 387.8; p < 0.0001 | |
| 2B (Nacc) | F(1,36) = 0.2597; p = 0.6134 | F(1,36) = 204.3; p < 0.0001 | F(1,36) = 97.83; p < 0.0001 | ||||
| Marble burying | CMV-Gpr88 | N = 9 CMV-CTL, N = 10 CMV-Gpr88 | 3A, left | t(17) = 2.03, p = 0.059 | |||
| D1R-Gpr88 | N = 14 D1R-CTL, N = 10 D1R-Gpr88 | 3B, left | t(22) = 1.002, p = 0.33 | ||||
| A2AR-Gpr88 | N = 10 A2AR-CTL, N = 10 A2AR-Gpr88 | 3C, left | t(18) = 4.01, p < 0.001 | ||||
| Nose contact in social interaction | CMV-Gpr88 | N = 8 CMV-CTL, N = 8 CMV-Gpr88 | 3D, right | t(14) = 2.88, p = 0.012 | |||
| D1R-Gpr88 | N = 14 D1R-CTL, N = 8 D1R-Gpr88 | 3E, right | t(20) = 2.57, p = 0.018 | ||||
| A2AR-Gpr88 | N = 10 A2AR-CTL, N = 10 A2AR-Gpr88 | 3F, right | t(18) = 2.06, p = 0.01 | ||||
| Open field (all sessions) | CMV-Gpr88 | N = 21 CMV-CTL, N = 21 CMV-Gpr88 | 4A, left | F(1,40) = 4.357; p = 0.0425 | F(4,180) = 4.419; p = 0.002 | F(4,180) = 7.189; p < 0.0001 | |
| D1R-Gpr88 | N = 13 D1R-CTL, N = 12 D1R-Gpr88 | 4B, left | F(1,23) = 1.106; p = 0.3038 | F(4,92) = 31.03; p < 0.0001 | F(4,92) = 11.82; p < 0.0001 | ||
| A2AR-Gpr88 | N = 17 A2AR-CTL, N = 10 A2AR-Gpr88 | 4C, left | F(1,25) = 8.004; p = 0.0091 | F(4,100) = 43.28; p < 0.0001 | F(4,100) = 3.939; p = 0.0052 | ||
| Open field (sessions 1 and 5) | CMV-Gpr88 | N = 21 CMV-CTL, N = 21 CMV-Gpr88 | 4A, right | F(1,80) = 4.93; p = 0.0292 | F(1,80) = 1.25;p = 0.2679 | F(1,80) = 8.94; p = 0.0037 | |
| D1R-Gpr88 | N = 13 D1R-CTL, N = 12 D1R-Gpr88 | 4B, right | F(1,46) = 0.78; p = 0.3811 | F(1,46) = 26.75;p < 0.0001 | F(1,46) = 11.01; p = 0.0018 | ||
| A2AR-Gpr88 | N = 17 A2AR-CTL, N = 10 A2AR-Gpr88 | 4C, right | F(1,50) = 8.17; p = 0.0062 | F(1,50) = 18.71; p < 0.0001 | F(1,50) = 0.1479; p = 0.7021 | ||
| Stereotypies | CMV-Gpr88 | N = 21 CMV-CTL, N = 21 CMV-Gpr88 | 5A | Score: t(40) = 2.228; p = 0.0316Time: t(40) = 2.818; p = 0.0075 | |||
| D1R-Gpr88 | N = 13 D1R-CTL, N = 12 D1R-Gpr88 | 5B | Score: t(23) = 1.156; p = 0.2594Time: t(23) = 0.7174; p = 0.4803 | ||||
| A2AR-Gpr88 | N = 17 A2AR-CTL, N = 10 A2AR-Gpr88 | 5C | Score: t(25) = 2.291; p = 0.0307Time: t(25) = 2.317; p = 0.0290 | ||||
| Rotarod (all sessions) | CMV-Gpr88 | N = 21 CMV-CTL, N = 21 CMV-Gpr88 | 6A, left | F(1,40) = 17.73; p = 0.0001 | F(23,920) = 13.49; p < 0.0001 | F(23,920) = 3.159; p < 0.0001 | |
| D1R-Gpr88 | N = 13 D1R-CTL, N = 12 D1R-Gpr88 | 6B, left | F(1,23) = 8.759; p = 0.0070 | F(23,529) = 10.09; p < 0.0001 | F(23,529) = 7.607; p < 0.0001 | ||
| A2AR-Gpr88 | N = 17 A2AR-CTL, N = 10 A2AR-Gpr88 | 6C, left | F(1,25) = 8.008; p = 0.0091 | F(23,575) = 13.74; p < 0.0001 | F(23,575) = 1.017; p = 0.4403 | ||
| Rotarod (sessions 1 and 6) | CMV-Gpr88 | N = 21 CMV-CTL, N = 21 CMV-Gpr88 | 6A, right | F(1,80) = 32.62; p < 0.0001 | F(1,80) = 17.67 p < 0.0001 | F(1,80) = 4.517; p = 0.0367 | |
| D1R-Gpr88 | N = 13 D1R-CTL, N = 12 D1R-Gpr88 | 6B, right | F(1,46) = 13.62; p = 0.0006 | F(1,46) = 11.15; p = 0.0017 | F(1,46) = 7.643; p = 0.0082 | ||
| A2AR-Gpr88 | N = 17 A2AR-CTL, N = 10 A2AR-Gpr88 | 6C, right | F(1,50) = 8.067; p = 0.0065 | F(1,50) = 16.31; p = 0.0002 | F(1,50) = 1.299; p = 0.2598 | ||