Decreased PMCA activity increases GECI calcium transients
| pH 7.3 | pH 8.8 | pH 7.3 | |
|---|---|---|---|
| Resting F (a.u.) | 583 ± 86 (22) | 661 ± 143 (22)*** | 580 ± 80 (22) |
| Peak (%ΔF/F) | 28 [18–71] (22) | 55 [25–84] (22)** | 23 [14–49] (22) |
| RT (s) | 1.21 ± 0.27 (21) | 1.63 ± 0.36 (21)**** | 1.17 ± 0.28 (21) |
| DT 1 (s) | 2.77 ± 0.82 (19) | 4.71**** ± 1.78 (19)**** | 2.22 ± 0.47 (19) |
| DT 2 (s) | 8.78 ± 0.99 (16) | 10.49** ± 2.30 (16)** | 7.88 ± 1.56 (16) |
Median [IQR] or mean ± SD. Parentheses indicate number of cells. Different from baseline: **p < 0.01, ***p < 0.001, ****p < 0.0001. Changes in calcium transient parameters in response to elevation of bath pH to block PMCA activity. Paired t tests revealed that parameter values significantly increase when bath pH is changed to pH 8.8 and recover when bath pH is returned to pH 7.3.