PT  - JOURNAL ARTICLE
AU  - Kent Imaizumi
AU  - Koki Fujimori
AU  - Seiji Ishii
AU  - Asako Otomo
AU  - Yasushi Hosoi
AU  - Hiroaki Miyajima
AU  - Hitoshi Warita
AU  - Masashi Aoki
AU  - Shinji Hadano
AU  - Wado Akamatsu
AU  - Hideyuki Okano
TI  - Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
AID  - 10.1523/ENEURO.0368-17.2018
DP  - 2018 Mar 01
TA  - eneuro
PG  - ENEURO.0368-17.2018
VI  - 5
IP  - 2
4099  - http://www.eneuro.org/content/5/2/ENEURO.0368-17.2018.short
4100  - http://www.eneuro.org/content/5/2/ENEURO.0368-17.2018.full
SO  - eNeuro2018 Mar 01; 5
AB  - The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling.