RT Journal Article
SR Electronic
T1 SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0175-17.2017
DO 10.1523/ENEURO.0175-17.2017
VO 4
IS 6
A1 Lei Zhang
A1 Angela Marie Jablonski
A1 Jelena Mojsilovic-Petrovic
A1 Hua Ding
A1 Steven Seeholzer
A1 Ian Paterson Newton
A1 Inke Nathke
A1 Rachael Neve
A1 JinBin Zhai
A1 Yuan Shang
A1 Mingjie Zhang
A1 Robert Gordon Kalb
YR 2017
UL http://www.eneuro.org/content/4/6/ENEURO.0175-17.2017.abstract
AB The dendritic tree is a key determinant of neuronal information processing. In the motor system, the dendritic tree of spinal cord neurons undergoes dramatic remodeling in an activity-dependent manner during early postnatal life. This leads to the proper segmental spinal cord connectivity that subserves normal locomotor behavior. One molecular system driving the establishment of dendrite architecture of mammalian motor neurons relies on AMPA receptors (AMPA-Rs) assembled with the GluA1 subunit, and this occurs in an NMDA receptor (NMDA-R)-independent manner. The dendrite growth promoting activity of GluA1-containing AMPA-Rs depends on its intracellular binding partner, SAP97, and SAP97’s PDZ3 domain. We show here that cysteine-rich interactor of PDZ3 (CRIPT) is a bona fide SAP97 PDZ3-domain binding partner, localizes to synapses with GluA1 and SAP97 along the dendritic tree, and is a determinant of the dendritic growth of mammalian spinal cord neurons. We further show that CRIPT has a well-conserved ortholog in the nematode, Caenorhabditis elegans, and animals lacking CRIPT display decreased dendrite branching of the well-studied PVD neuron in vivo. The lack of CRIPT leads to a selective defect in touch perception, and this is rescued by expression of wild-type (WT) human CRIPT (hCRIPT) in the nervous system. This work brings new light into the molecular machinery that drives dendritic growth during development and may prove relevant to the promotion of nervous system plasticity following insult.