TY - JOUR T1 - Cross-Laboratory Analysis of Brain Cell Type Transcriptomes with Applications to Interpretation of Bulk Tissue Data JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0212-17.2017 VL - 4 IS - 6 SP - ENEURO.0212-17.2017 AU - B. Ogan Mancarci AU - Lilah Toker AU - Shreejoy J. Tripathy AU - Brenna Li AU - Brad Rocco AU - Etienne Sibille AU - Paul Pavlidis Y1 - 2017/11/01 UR - http://www.eneuro.org/content/4/6/ENEURO.0212-17.2017.abstract N2 - Establishing the molecular diversity of cell types is crucial for the study of the nervous system. We compiled a cross-laboratory database of mouse brain cell type-specific transcriptomes from 36 major cell types from across the mammalian brain using rigorously curated published data from pooled cell type microarray and single-cell RNA-sequencing (RNA-seq) studies. We used these data to identify cell type-specific marker genes, discovering a substantial number of novel markers, many of which we validated using computational and experimental approaches. We further demonstrate that summarized expression of marker gene sets (MGSs) in bulk tissue data can be used to estimate the relative cell type abundance across samples. To facilitate use of this expanding resource, we provide a user-friendly web interface at www.neuroexpresso.org. ER -