RT Journal Article SR Electronic T1 Cross-Laboratory Analysis of Brain Cell Type Transcriptomes with Applications to Interpretation of Bulk Tissue Data JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0212-17.2017 DO 10.1523/ENEURO.0212-17.2017 VO 4 IS 6 A1 B. Ogan Mancarci A1 Lilah Toker A1 Shreejoy J. Tripathy A1 Brenna Li A1 Brad Rocco A1 Etienne Sibille A1 Paul Pavlidis YR 2017 UL http://www.eneuro.org/content/4/6/ENEURO.0212-17.2017.abstract AB Establishing the molecular diversity of cell types is crucial for the study of the nervous system. We compiled a cross-laboratory database of mouse brain cell type-specific transcriptomes from 36 major cell types from across the mammalian brain using rigorously curated published data from pooled cell type microarray and single-cell RNA-sequencing (RNA-seq) studies. We used these data to identify cell type-specific marker genes, discovering a substantial number of novel markers, many of which we validated using computational and experimental approaches. We further demonstrate that summarized expression of marker gene sets (MGSs) in bulk tissue data can be used to estimate the relative cell type abundance across samples. To facilitate use of this expanding resource, we provide a user-friendly web interface at www.neuroexpresso.org.